Two-stage tuberculosis diagnostics: combining centrifugal microfluidics to detect TB infection and Inh and Rif resistance at the point of care with subsequent antibiotic resistance profiling by targeted NGS
Judith Schlanderer,
Harald Hoffmann,
Jan Lüddecke
et al.
Abstract:Globally, tuberculosis (TB) remains the deadliest bacterial infectious disease and spreading antibiotic resistances is the biggest challenge for combatting the disease. Rapid and comprehensive diagnostics including drug susceptibility testing (DST)...
“…These methods can be integrated into microfluidic chips to automate DNA extraction as has already been demonstrated for various applications. 28–30 Other methods comprise the enrichment of DNA 31,32 or simple two step crude extraction. However, none of these approaches was developed for the analysis of meat products focusing on minimal hands on time, a short time-to-result and the use of simple standard laboratory equipment.…”
“…These methods can be integrated into microfluidic chips to automate DNA extraction as has already been demonstrated for various applications. 28–30 Other methods comprise the enrichment of DNA 31,32 or simple two step crude extraction. However, none of these approaches was developed for the analysis of meat products focusing on minimal hands on time, a short time-to-result and the use of simple standard laboratory equipment.…”
This review explores emerging microfluidic-based technologies incorporating innovative assay strategies for infectious disease diagnostics. Our classification scheme is based on the human body systems or pathogen transmission modes.
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