Two SPRi biosensors for the determination of cathepsin S in blood plasma

Ołdak, Łukasz; Sankiewicz, Anna; Żelazowska-Rutkowska, Beata; Cylwik, Bogdan; Łukaszewski, Zenon; Skoczylas, Marcin; Gorodkiewicz, Ewa

doi:10.1016/j.talanta.2020.121900

Cited by 10 publications

(7 citation statements)

References 25 publications

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“…The dependence of the analytical signal on the analyte concentration was examined within the range 0.1–3.5 ng mL −1 . The optimum concentration of the rat monoclonal antibody specific for cathepsin S was selected in a previous study [ 2 ], and was fixed at 20 ng mL −1 with pH = 7.4. The results are shown in Figure 5 .…”

Section: Resultsmentioning

confidence: 99%

“…First, the Ag/Au and Au chips were covered with a cysteamine linker. Antibody immobilization was performed following the well-known EDS/NHS protocol, described in a previous paper [ 2 ], with the rat monoclonal antibody specific for cathepsin S.…”

Section: Apparatus Chip Manufacture Materials and Methodsmentioning

confidence: 99%

“…Among SPR techniques, the array SPR imaging (SPRi) is well suited to this purpose. Biosensors for the determination of cathepsins B, D, G [ 1 ], L, and S [ 2 ], laminin-5 [ 3 ], fibronectin [ 4 ], collagen IV [ 5 ], proteasome 20S and immunoproteasome 20S, UCHL-1 [ 6 ], MMP-1 [ 7 ] and MMP-2 [ 8 ], aromatase, podoplanin, leptin, CA 125, CEA, HE 4, and cystatin C in body fluids have been developed [ 9 ]. Many of these biosensors have been used in clinical investigations, including those of bladder cancer [ 10 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

“…The aim of this work was to investigate the analytical characteristics of a biosensor formed on a Ag/Au chip and to compare them with those for a biosensor formed on a gold chip. A recently developed biosensor for the determination of cathepsin S (Cath S) was chosen as an example [ 2 ]. This biosensor was used in a previous paper [ 17 ] for an initial examination of the analytical potential of this biosensor.…”

Section: Introductionmentioning

confidence: 99%

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An Immunosensor for the Determination of Cathepsin S in Blood Plasma by Array SPRi—A Comparison of Analytical Properties of Silver–Gold and Pure Gold Chips

et al. 2021

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The array SPR imaging (SPRi) technique is well suited to the determination of biomarkers in body fluids, called liquid biopsy. No signal enhancement or analyte preconcentration is required. With the aim of achieving signal enhancement and lowering the cost of a single determination, the replacement of gold-covered chips by silver–gold chips was investigated. The aim of this work was to investigate the analytical characteristics of a biosensor formed on a Ag/Au chip and to compare them with those of a biosensor formed on a gold chip. A biosensor for the determination of cathepsin S (Cath S) was chosen as an example. The biosensor consisted of the linker cysteamine and an immobilized rat monoclonal antibody specific for cathepsin S. Both biosensors exhibited a Langmuirian response to Cath S concentration, with linear response ranging from LOQ to 1.5 ng mL−1. The LOQ is 0.1 ng mL−1 for the biosensor formed on the Ag/Au chip, and 0.22 ng mL−1 for that formed on the gold chip. Recoveries and precision for medium and high Cath S concentrations were acceptable for both biosensors, i.e., precision better than 10% and recoveries within the range 102–105%. However, the results for the lowest Cath S concentration were better for the biosensor formed on the Ag/Au chip (9.4 and 106% for precision and recovery, respectively). Generally, no significant differences in analytical characteristics were observed between the Ag/Au and Au chips. The two biosensors were also compared in the determination of Cath S in real samples. Nine plasma samples from healthy donors and nine from patients with ovarian cancer were analyzed for Cath S concentration with the biosensors formed on Ag/Au and Au chips. The results obtained with the two biosensors were very similar and show no significant differences on the Bland–Altman plot. The Cath S concentration in the blood plasma of ovarian cancer patients was elevated by one order of magnitude as compared with the control (12.6 ± 3.6 vs. 1.6 ± 1.2 ng mL−1).

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Section: Resultsmentioning

confidence: 99%

Section: Apparatus Chip Manufacture Materials and Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

An Immunosensor for the Determination of Cathepsin S in Blood Plasma by Array SPRi—A Comparison of Analytical Properties of Silver–Gold and Pure Gold Chips

et al. 2021

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“…Apart from bifunctional molecules, simple n -alkanethiols are also used in the construction of SPR sensors. Layers based on n -octanethiol were employed to passivate a gold surface or immobilize low-molecular, non-polar inhibitors, and antigens of non-protein nature by hydrophobic interactions [ 54 , 55 ]. Various strategies exploiting thiol-based SAMs in the construction of immunosensors for the detection of clinically relevant analytes are presented in Figure 2 .…”

Section: Functional Surfaces and Non-oriented Ab Immobilization Strategies For Spr Immunosensingmentioning

confidence: 99%

Recent Advancements in Receptor Layer Engineering for Applications in SPR-Based Immunodiagnostics

Drozd

Karoń

Malinowska

2021

Sensors

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The rapid progress in the development of surface plasmon resonance-based immunosensing platforms offers wide application possibilities in medical diagnostics as a label-free alternative to enzyme immunoassays. The early diagnosis of diseases or metabolic changes through the detection of biomarkers in body fluids requires methods characterized by a very good sensitivity and selectivity. In the case of the SPR technique, as well as other surface-sensitive detection strategies, the quality of the transducer-immunoreceptor interphase is crucial for maintaining the analytical reliability of an assay. In this work, an overview of general approaches to the design of functional SPR-immunoassays is presented. It covers both immunosensors, the design of which utilizes well-known and often commercially available substrates, as well as the latest solutions developed in-house. Various approaches employing chemical and passive binding, affinity-based antibody immobilization, and the introduction of nanomaterial-based surfaces are discussed. The essence of their influence on the improvement of the main analytical parameters of a given immunosensor is explained. Particular attention is paid to solutions compatible with the latest trends in the development of label-free immunosensors, such as platforms dedicated to real-time monitoring in a quasi-continuous mode, the use of in situ-generated receptor layers (elimination of the regeneration step), and biosensors using recombinant and labelled protein receptors.

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The SPRi determination of cathepsin L and S in plasma and peritoneal fluid of women with endometriosis

Załęcka,

Zielińska,

Ołdak

et al. 2024

Advances in Medical Sciences

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Two SPRi biosensors for the determination of cathepsin S in blood plasma

Cited by 10 publications

References 25 publications

An Immunosensor for the Determination of Cathepsin S in Blood Plasma by Array SPRi—A Comparison of Analytical Properties of Silver–Gold and Pure Gold Chips

An Immunosensor for the Determination of Cathepsin S in Blood Plasma by Array SPRi—A Comparison of Analytical Properties of Silver–Gold and Pure Gold Chips

Recent Advancements in Receptor Layer Engineering for Applications in SPR-Based Immunodiagnostics

The SPRi determination of cathepsin L and S in plasma and peritoneal fluid of women with endometriosis

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