Two sporulated Bacillus enhance immunity in Galleria mellonella protecting against Candida albicans

Barros, Patrícia Pimentel de; Rossoni, Rodnei Dennis; Ribeiro, Felipe de Camargo; Silva, Michelle Peneluppi; Souza, Cheyenne Marçal de; Jorge, Antônio Olavo Cardoso

doi:10.1016/j.micpath.2019.05.023

Cited by 16 publications

(11 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Interestingly, a similar observation was previously made in mouse macrophages where an H 2 O 2 burst was detected 45 min after Salmonella infection (Aussel et al, 2011). We did not investigate the role of phagocytosis during the infection but we showed that an H 2 O 2 burst was produced by Galleria and sensed by Salmonella 30 min post-infection.…”

supporting

confidence: 80%

“…This lepidopteran was first used in the 1960s to test the virulence of a wide variety of microorganisms, from bacteria to fungi and viruses (Kurstak and Vega, 1968;Lysenko and Kucera, 1968;Younghusband and Lee, 1969). Over the last decade, it has become an attractive model in the field of host-pathogen interactions (Repizo et al, 2015;Cools et al, 2018;Pereira et al, 2018;Sciuto et al, 2018;Candela et al, 2019;Barros et al, 2019). And more recently, a high-throughput screening was carried out in G. mellonella to evaluate the synergy between antibiotics, human drugs and food additives on a various bacteria (Brochado et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Bacterial Genetic Approach to the Study of Reactive Oxygen Species Production in Galleria mellonella During Salmonella Infection

Bismuth

Brasseur

Ezraty

et al. 2021

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

Over the last decade, an increasing number of reports presented Galleria mellonella larvae as an important model to study host-pathogen interactions. Coherently, increasing information became available about molecular mechanisms used by this host to cope with microbial infections but few of them dealt with oxidative stress. In this work, we addressed the role of reactive oxygen species (ROS) produced by the immune system of G. mellonella to resist against Salmonella enterica, an intracellular pathogen responsible for a wide range of infections. We confirmed that Salmonella was pathogen for G. mellonella and showed that it had to reach a minimal bacterial load within the hemolymph to kill the larvae. ROS production by G. mellonella was revealed by the virulence defects of Salmonella mutants lacking catalases/peroxiredoxins or cytoplasmic superoxide dismutases, both strains being highly sensitive to these oxidants. Finally, we used bacterial transcriptional fusions to demonstrate that hydrogen peroxide (H2O2) was produced in the hemolymph of Galleria during infection and sensed by S. enterica. In line with this observation, the H2O2-dependent regulator OxyR was found to be required for bacterial virulence in the larvae. These results led us to conclude that ROS production is an important mechanism used by G. mellonella to counteract bacterial infections and validate this host as a relevant model to study host-pathogen interactions.

show abstract

supporting

confidence: 80%

Section: Introductionmentioning

confidence: 99%

Bacterial Genetic Approach to the Study of Reactive Oxygen Species Production in Galleria mellonella During Salmonella Infection

Bismuth

Brasseur

Ezraty

et al. 2021

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

show abstract

“…Another study demonstrated that sublethal doses of C. albicans and A. fumigatus induced the expression of the same antimicrobial peptides [50,61]. Furthermore, the infection of G. mellonella with Bacillus subtilis and Bacillus atrophaeus vegetative and sporulating forms also increased gallerimycin and galliomycin expression [69]. The pre-infection with B. subtilis and B. atrophaeus increased gallerimycin and galiomicin expression and survival of larvae infected with C. albicans.…”

Section: Discussionmentioning

confidence: 95%

In Vitro and In Vivo Effect of Peptides Derived from 14-3-3 Paracoccidioides spp. Protein

Scorzoni

Silva

Oliveira

et al. 2021

JoF

View full text Add to dashboard Cite

Background: Paracoccidioidomycosis (PCM) is a chronic disease that causes sequelae and requires prolonged treatment; therefore, new therapeutic approaches are necessary. In view of this, three peptides from Paracoccidioides brasiliensis 14-3-3 protein were selected based on its immunogenicity and therapeutic potential. Methods: The in vitro antifungal activity and cytotoxicity of the 14-3-3 peptides were evaluated. The influence of the peptides in immunological and survival aspects was evaluated in vivo, using Galleria mellonella and the expression of antimicrobial peptide genes in Caenorhabditis elegans. Results: None of the peptides were toxic to HaCaT (skin keratinocyte), MRC-5 (lung fibroblast), and A549 (pneumocyte) cell lines, and only P1 exhibited antifungal activity against Paracoccidioides spp. The peptides could induce an immune response in G. mellonella. Moreover, the peptides caused a delay in the death of Paracoccidioides spp. infected larvae. Regarding C. elegans, the three peptides were able to increase the expression of the antimicrobial peptides. These peptides had essential effects on different aspects of Paracoccidioides spp. infection showing potential for a therapeutic vaccine. Future studies using mammalian methods are necessary to validate our findings.

show abstract

“…To investigate the ability of anidulafungin liposomes and free drug to improve survival of C. albicans SC5314–infected G. mellonella wax moth larvae, the methodology described by de Barros et al was used with some modifications (de Barros et al, 2019). Twelve randomly chosen G. mellonella larvae of similar weight and size (250–350 mg) were used per group in all assays.…”

Section: Methodsmentioning

confidence: 99%

Anidulafungin liposome nanoparticles exhibit antifungal activity against planktonic and biofilm Candida albicans

Vera‐González

Bailey‐Hytholt

Langlois

et al. 2020

J Biomedical Materials Res

Self Cite

View full text Add to dashboard Cite

Fungal infections can cause significant patient morbidity and mortality. Nanoparticle therapeutics have the potential to improve treatment of these infections. Here we report the development of liposomal nanoparticles incorporating anidulafungin, a potent antifungal, with the goal of increasing its solubility and aiding in localization to fungi. Liposomes were fabricated with three concentrations of anidulafungin yielding monodisperse ~100 nm unilamellar vesicles. All three formulations inhibited planktonic Candida albicans growth at a minimum inhibitory concentration equivalent to free drug. All three formulations also disrupted preformed C. albicans biofilms, reducing fungal burden by as much as 99%, exhibiting superior biofilm disruption compared with free drug. Liposome formulations tested in vivo in C. albicans infected Galleria mellonella wax moth larvae demonstrated increased survival compared to free drug equivalents, leading to a survival of 33 to 67% of larvae over 7 days depending on the liposome utilized compared with only 25% survival of larvae administered free drug. Liposomal formulations along with free anidulafungin did not cause red blood cell lysis. Ultimately, the liposome formulations reported here increased anidulafungin solubility, displayed promising efficacy against planktonic and biofilm C. albicans, and improved the survival of C. albicans–infected G. mellonella compared to free anidulafungin.

show abstract

Two sporulated Bacillus enhance immunity in Galleria mellonella protecting against Candida albicans

Cited by 16 publications

References 48 publications

Bacterial Genetic Approach to the Study of Reactive Oxygen Species Production in Galleria mellonella During Salmonella Infection

Bacterial Genetic Approach to the Study of Reactive Oxygen Species Production in Galleria mellonella During Salmonella Infection

In Vitro and In Vivo Effect of Peptides Derived from 14-3-3 Paracoccidioides spp. Protein

Anidulafungin liposome nanoparticles exhibit antifungal activity against planktonic and biofilm Candida albicans

Contact Info

Product

Resources

About