Two Species of Dermatan Sulfate Proteoglycans with Different Molecular Sizes from Newborn Calf Skin

Deficiency of the core proteins of dermatan sulphate proteoglycans in a variant form of Ehlers‐Danlos syndrome

“…Frozen sections (6 μm thick) of the control's and of the patient's skin, prepared using a cryostat microtome, were air dried and fixed in cold acetone for 20 min. For immunofluorescent staining, anti‐core proteins of DS‐PGs rabbit serum were prepared [8]. The sections were incubated with anti‐serum diluted 1:100 at 37 °C for 30 min.…”

The Proteoglycans in Hypertrophic Scar

“…Although almost all hyaluronic acid was extractable with 6 M urea, the remaining glycosaminoglycan were co-precipitated with insoluble tissues. The extraction procedure employed yielded quantities of glycosaminoglycans similar to those in a previous study (10). Two types of proteoglycans, proteodermatan sulfate and dermatan sulfate containing, chondroitin sulfate rich proteoglycan, were obtained from hypertrophic scar.…”

“…The functions of such Nlinked oligosaccharide chains on core protein are not known, but dermatan sulfate containing chondroitin sulfate rich proteoglycans which did not bind to concanavalin A Sepharose were recovered as major components of hypertrophic scar. In addition, small amounts of dermatan sulfate/chondroitin sulfate proteoglycans composed of Mr=20,000 core protein, which did not bind to concanavalin A, were present in normal calf skin (10). Recently, a similar proteoglycan that consisted of 66% dermatan sulfate, 20% chondroitin sulfate, and Mr=45,000 core protein was isolated from human fetal membranes; it has a Mr=120,000 (22).…”

“…Proteodermatan sulfate, to which dermatan sulfate is covalently bound with the Mr=55,000 core protein, has been extracted as a major proteoglycan by neutral salt (9) or 4M-guanidinium chlori<lf. As a minor component, chondroitin sulfate/dermatan sulfate containing proteoglycans, composed of Mr=20,000 core protein, were extracted from newborn calf skin (10). To investigate the fibrillogenesis of hypertrophic scar, proteoglycans of the tissue were characterized.…”

Pharmacology of the Skin I

“…The polypeptide core of proteodermatan sulphate exists in two different sizes (MATSUNAGA and SHINKAI 1986). Although most of the information about polysaccharides (an old term) arises from the study of cartilage, it is most probable that it in part applies to skin.…”