Two RNA Binding Proteins, HEN4 and HUA1, Act in the Processing of AGAMOUS Pre-mRNA in Arabidopsis thaliana

Cheng, Yulan; Kato, Naohiro; Wang, Wenming; Li, Junjie; Chen, Xuemei

doi:10.1016/s1534-5807(02)00399-4

Cited by 165 publications

(206 citation statements)

References 67 publications

(55 reference statements)

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“…The presence of the CCCH-type zinc fingers in OsDOS provides a clue to its potential molecular function. Many proteins with the similar zinc fingers have been shown to be RNA-binding proteins and to be associated with RNA metabolism in various organisms, such as the murine TTP (Carballo et al, 1998(Carballo et al, , 2000 and the Arabidopsis HUA1 (Li et al, 2001;Cheng et al, 2003). Thus, based on the nuclear localization of the OsDOS protein, we propose that OsDOS possibly plays a role at posttranscriptional level in delaying leaf senescence in rice.…”

Section: The Ja Pathway Is Involved In the Osdos-mediated Senescencementioning

confidence: 89%

“…Best known among them is the TTP, which is an RNA-binding protein that binds to AU-rich elements in the 3# untranslated regions of tumor necrosis factor-a and granulocytemacrophage colony-stimulating factor mRNAs for degradation of the messages, and thus regulates the level of protein expression (Carballo et al, 1998(Carballo et al, , 2000. In Arabidopsis, HUA1 functions in floral reproductive organ identity by binding AGAMOUS pre-mRNA and facilitating its processing (Li et al, 2001;Cheng et al, 2003).…”

Section: Identification and Structural Features Of Osdos Proteinmentioning

confidence: 99%

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A Novel Nuclear-Localized CCCH-Type Zinc Finger Protein, OsDOS, Is Involved in Delaying Leaf Senescence in Rice

et al. 2006

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Leaf senescence is a developmentally programmed degeneration process, which is fine tuned by a complex regulatory network for plant fitness. However, molecular regulation of leaf senescence is poorly understood, especially in rice (Oryza sativa), an important staple crop for more than half of the world population. Here, we report a novel nuclear-localized CCCH-type zinc finger protein, Oryza sativa delay of the onset of senescence (OsDOS), involved in delaying leaf senescence in rice. The expression of OsDOS was down-regulated during natural leaf senescence, panicle development, and pollination, although its transcripts were accumulated in various organs. RNAi knockdown of OsDOS caused an accelerated age-dependent leaf senescence, whereas its overexpression produced a marked delay of leaf senescence, suggesting that it acts as a negative regulator for leaf senescence. A genome-wide expression analysis further confirmed its negative regulation for leaf senescence and revealed that, in particular, the jasmonate (JA) pathway was found to be hyperactive in the OsDOS RNAi transgenic lines but impaired in the OsDOS overexpressing transgenic lines, indicating that this pathway is likely involved in the OsDOS-mediated delaying of leaf senescence. Furthermore, methyl JA treatments of both seeds and detached leaves from the RNAi and the overexpressing transgenic lines showed hyperand hyporesponses, respectively, consistent with the negative regulation of the JA pathway by OsDOS. Together, these results indicate that OsDOS is a novel nuclear protein that delays leaf senescence likely, at least in part, by integrating developmental cues to the JA pathway.

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Section: The Ja Pathway Is Involved In the Osdos-mediated Senescencementioning

confidence: 89%

Section: Identification and Structural Features Of Osdos Proteinmentioning

confidence: 99%

A Novel Nuclear-Localized CCCH-Type Zinc Finger Protein, OsDOS, Is Involved in Delaying Leaf Senescence in Rice

et al. 2006

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“…Both proteins, together with HUA ENHANCER (HEN) 2 and 4 (that were themselves identified in a screen for enhancers of hua1 hua2) are involved in the control of AG pre-mRNA correct splicing (Cheng et al, 2003).…”

Section: Fm Termination Relies On a Complex Multilevel Activation Of Agmentioning

confidence: 99%

Time to Stop: Flower Meristem Termination

Prunet¹,

Morel²,

Negrutiu³

et al. 2009

Plant Physiol.

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“…Most of the characterized CCCH-type zinc finger proteins are associated with RNA metabolism by binding to the target mRNA (Cheng et al, 2003;Delaney et al, 2006;Lai et al, 2006;Hurt et al, 2009), and only two, Arabidopsis PEI1 and rice OsLIC, are suggested to transcriptionally regulate gene expression by binding to DNA (Li and Thomas, 1998;Wang et al, 2008b). In addition, OsLIC harbors an EELR-type activation domain for regulating gene transcription in yeast (Wang et al, 2008b).…”

Section: C3h12 May Function As An Rna-binding Proteinmentioning

confidence: 99%

“…In Arabidopsis, the CCCH-type protein HUA1 is involved in the processing of AGAMOUS pre-mRNA as an RNAbinding protein during flower development (Li et al, 2001;Cheng et al, 2003). Another Arabidopsis CCCHtype protein, AtTZF1, shuttling between the nucleus and cytoplasmic foci, can bind both DNA and RNA in vitro and is likely involved in GA/abscisic acidmediated developmental and environmental responses through DNA or RNA regulation (Pomeranz et al, 2010).…”

mentioning

confidence: 99%

A CCCH-Type Zinc Finger Nucleic Acid-Binding Protein Quantitatively Confers Resistance against Rice Bacterial Blight Disease

et al. 2011

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Bacterial blight is a devastating disease of rice (Oryza sativa) caused by Xanthomonas oryzae pv oryzae (Xoo). Zinc finger proteins harboring the motif with three conserved cysteine residues and one histidine residue (CCCH) belong to a large family. Although at least 67 CCCH-type zinc finger protein genes have been identified in the rice genome, their functions are poorly understood. Here, we report that one of the rice CCCH-type zinc finger proteins, C3H12, containing five typical CX 8 -CX 5 -CX 3 -H zinc finger motifs, is involved in the rice-Xoo interaction. Activation of C3H12 partially enhanced resistance to Xoo, accompanied by the accumulation of jasmonic acid (JA) and induced expression of JA signaling genes in rice. In contrast, knockout or suppression of C3H12 resulted in partially increased susceptibility to Xoo, accompanied by decreased levels of JA and expression of JA signaling genes in rice. C3H12 colocalized with a minor disease resistance quantitative trait locus to Xoo, and the enhanced resistance of randomly chosen plants in the quantitative trait locus mapping population correlated with an increased expression level of C3H12. The C3H12 protein localized in the nucleus and possessed nucleic acid-binding activity in vitro. These results suggest that C3H12, as a nucleic acid-binding protein, positively and quantitatively regulates rice resistance to Xoo and that its function is likely associated with the JA-dependent pathway.

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Two RNA Binding Proteins, HEN4 and HUA1, Act in the Processing of AGAMOUS Pre-mRNA in Arabidopsis thaliana

Cited by 165 publications

References 67 publications

A Novel Nuclear-Localized CCCH-Type Zinc Finger Protein, OsDOS, Is Involved in Delaying Leaf Senescence in Rice

A Novel Nuclear-Localized CCCH-Type Zinc Finger Protein, OsDOS, Is Involved in Delaying Leaf Senescence in Rice

Time to Stop: Flower Meristem Termination

A CCCH-Type Zinc Finger Nucleic Acid-Binding Protein Quantitatively Confers Resistance against Rice Bacterial Blight Disease

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