Two rice MYB transcription factors maintain male fertility in response to photoperiod by modulating sugar partitioning

Wang, Duoxiang; Li, Jingbin; Sun, Liqing; Hu, Yangyang; Yu, Jing; Wang, Canhua; Zhang, Fengli; Hou, Haili; Liang, Wanqi; Zhang, Dabing

doi:10.1111/nph.17512

Cited by 22 publications

(27 citation statements)

References 69 publications

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“…In Arabidopsis, AtMYB33 is involved in tapetum differentiation and AtMYB103 / 80 can participate in the late stage of tapetum development [ 39 , 40 , 41 ]. In rice, two rice MYB proteins, OsCSA2 and OsCSA, control male sterility by affecting the sugar partitioning in rice anthers [ 42 , 43 ]. Therefore, we searched for differentially expressed TFs in our transcriptome data.…”

Section: Resultsmentioning

confidence: 99%

Transcriptomics-Based Identification of Genes Related to Tapetum Degradation and Microspore Development in Lily

Sui

Jia

Yin

et al. 2022

Genes

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Lily is a popular and economically ornamental crop around the world. However, its high production of pollen grains causes serious problems to consumers, including allergies and staining of clothes. During anther development, the tapetum is a crucial step for pollen formation and microspore release. Therefore, it is important to understand the mechanism of tapetum degradation and microspore development in lily where free pollen contamination occurs. Here, we used the cut lily cultivar ‘Siberia’ to characterize the process of tapetum degradation through the use of cytology and transcriptomic methods. The cytological observation indicated that, as the lily buds developed from 4 cm (Lo 4 cm) to 8 cm (Lo 8 cm), the tapetum completed the degradation process and the microspores matured. Furthermore, by comparing the transcriptome profiling among three developmental stages (Lo 4 cm, Lo 6 cm and Lo 8 cm), we identified 27 differentially expressed genes. These 27 genes were classed into 4 groups by function, namely, cell division and expansion, cell-wall morphogenesis, transcription factors, LRR-RLK (leucine-rich repeat receptor-like kinases), plant hormone biosynthesis and transduction. Quantitative real-time PCR was performed as validation of the transcriptome data. These selected genes are candidate genes for the tapetum degradation and microspore development of lily and our work provides a theoretical basis for breeding new lily cultivars without pollen.

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Section: Resultsmentioning

confidence: 99%

Transcriptomics-Based Identification of Genes Related to Tapetum Degradation and Microspore Development in Lily

Sui

Jia

Yin

et al. 2022

Genes

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“…The csa mutant reveals a photoperiod-sensitive genic male sterile phenotype due to the disruption of sugar supply to the anther under different photoperiods: male-sterile under short-day conditions but only semi-sterile under long-day conditions [ 11 , 12 ]. Interestingly, CSA2, another MYB transcription factor, reveals the reverse phenotypes, being fertile under short-day and semi-sterile under long-day conditions [ 13 ]. A further study suggests that CSA2 shares common downstream genes involved in sugar metabolism with CSA including OsSWEET6a , OsINV4 , OsAZT3 , OsSPT17 , and OsSPT27 ( Figure 3 ) [ 13 , 44 ].…”

Section: Sugar Regulatory Networkmentioning

confidence: 99%

“…Interestingly, CSA2, another MYB transcription factor, reveals the reverse phenotypes, being fertile under short-day and semi-sterile under long-day conditions [ 13 ]. A further study suggests that CSA2 shares common downstream genes involved in sugar metabolism with CSA including OsSWEET6a , OsINV4 , OsAZT3 , OsSPT17 , and OsSPT27 ( Figure 3 ) [ 13 , 44 ].…”

Section: Sugar Regulatory Networkmentioning

confidence: 99%

“…Sugars are the constituents of main anther, and play essential roles in cell structure formation, energy supply, and male fertility in response to environmental conditions [ 9 ]. In rice, the expression of Cell Wall Invertase 3 ( OsCWIN3/OsINV4 ) correlates with sucrose accumulation and pollen sterility depending on temperature [ 10 ], while two MYB domain proteins, Carbon Starved Anther (CSA) and CSA2, regulate sugar partitioning and male fertility in response to photoperiod [ 11 , 12 , 13 , 14 ]. The sugar transporter OsXa13/OsSWEET11 plays essential roles in pollen development and disease resistance against bacterial blight [ 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

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Source-To-Sink Transport of Sugar and Its Role in Male Reproductive Development

Kim

Zhang

2022

Genes

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Sucrose is produced in leaf mesophyll cells via photosynthesis and exported to non-photosynthetic sink tissues through the phloem. The molecular basis of source-to-sink long-distance transport in cereal crop plants is of importance due to its direct influence on grain yield—pollen grains, essential for male fertility, are filled with sugary starch, and rely on long-distance sugar transport from source leaves. Here, we overview sugar partitioning via phloem transport in rice, especially where relevant for male reproductive development. Phloem loading and unloading in source leaves and sink tissues uses a combination of the symplastic, apoplastic, and/or polymer trapping pathways. The symplastic and polymer trapping pathways are passive processes, correlated with source activity and sugar gradients. In contrast, apoplastic phloem loading/unloading involves active processes and several proteins, including SUcrose Transporters (SUTs), Sugars Will Eventually be Exported Transporters (SWEETs), Invertases (INVs), and MonoSaccharide Transporters (MSTs). Numerous transcription factors combine to create a complex network, such as DNA binding with One Finger 11 (DOF11), Carbon Starved Anther (CSA), and CSA2, which regulates sugar metabolism in normal male reproductive development and in response to changes in environmental signals, such as photoperiod.

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“…The R2R3-MYB TFs in rice exerted an important effect in plant development and abiotic stress response. In development, some MYB TFs have been reported to affect male fertility [ 14 , 15 ], hull and grain development [ 16 ], and flower and spikelet development [ 17 ] of rice. In addition, it also showed a significant effect on salt [ 18 ], drought [ 19 ], and cold [ 20 ] resistance and heavy metal [ 21 ] tolerance in rice.…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Identification of R2R3-MYB Transcription Factor and Expression Analysis under Abiotic Stress in Rice

Kang

Teng

Cen

et al. 2022

Plants

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The myeloblastosis (MYB) family comprises a large group of transcription factors (TFs) that has a variety of functions. Among them, the R2R3-MYB type of proteins are the largest group in plants, which are involved in controlling various biological processes such as plant growth and development, physiological metabolism, defense, and responses to abiotic and biotic stresses. In this study, bioinformatics was adopted to conduct genome-wide identification of the R2R3-MYB TFs in rice. We identified 190 MYB TFs (99 R2R3-MYBs), which are unevenly distributed on the 12 chromosomes of rice. Based on the phylogenetic clustering and protein sequence characteristics, OsMYBs were classified into five subgroups, and 59.6% of the Os2R_MYB genes contained two introns. Analysis of cis-acting elements in the 2000 bp upstream region of Os2R_MYB genes showed that all Os2R_MYB genes contained plant hormones-related or stress-responsive elements since 91.9%, 79.8%, 79.8%, and 58.6% of Os2R_MYB genes contain ABRE, TGACG, CGTCA, and MBS motifs, respectively. Protein–protein network analysis showed that the Os2R_MYBs were involved in metabolic process, biosynthetic process, and tissue development. In addition, some genes showed a tissue-specific or developmental-stage-specific expression pattern. Moreover, the transcription levels of 20 Os2R_MYB genes under polyethylene glycol (PEG) and cadmium chloride (CdCl2) stress inducers were dissected by qRT-PCR. The results indicated genes with an altered expression upon PEG or CdCl2 stress induction. These results potentially supply a basis for further research on the role that Os2R_MYB genes play in plant development and stress responses.

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Two rice MYB transcription factors maintain male fertility in response to photoperiod by modulating sugar partitioning

Cited by 22 publications

References 69 publications

Transcriptomics-Based Identification of Genes Related to Tapetum Degradation and Microspore Development in Lily

Transcriptomics-Based Identification of Genes Related to Tapetum Degradation and Microspore Development in Lily

Source-To-Sink Transport of Sugar and Its Role in Male Reproductive Development

Genome-Wide Identification of R2R3-MYB Transcription Factor and Expression Analysis under Abiotic Stress in Rice

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