Two Prenylated and C-Methylated Flavonoids from<i>Tripterygium wilfordii</i>

Zeng, Feng; Wang, Wei; Wu, Yunshan; Dey, Moul; Ye, Min; Avery, Mitchell A.; Khan, Ikhlas A.; Guo, Dean

doi:10.1055/s-0029-1241017

Cited by 18 publications

(4 citation statements)

References 16 publications

(17 reference statements)

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“…wilfordii, TwUGT3 obtained from T. wilfordii has a strong specificity for flavonoids and is of great significance . Therefore, the construction of a compound biosynthetic chassis cell for industrial production should not be limited to the species in which the compound is located.…”

Section: Discussionmentioning

confidence: 99%

Investigating the Catalytic Activity of Glycosyltransferase on Quercetin from Tripterygium wilfordii

Gao

Lü

et al. 2020

ACS Omega

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Flavonoid glycosides have shown many pharmacological activities in clinical studies. However, the main way to obtain flavonoid glycosides is to extract and separate them from plants, which wastes both time and resources. Here, we identified the O-glycosyltransferase (UGTs) TwUGT3 from Tripterygium wilfordii and analyzed its bioinformatics. First, the enzyme was found to utilize phloretin and uridine diphosphate glucose (UDPG) as substrates to produce an acid-tolerant glucoside. Then, it also can use quercetin and UDPG as substrates to produce the corresponding O-glucoside. In addition, we further explored the substrate specificity of TwUGT3, which suggested that it also accepts luteolin, pinocembrin, and genistein to produce the corresponding Oglucosides. Subsequently, the optimum pH, reaction time, reaction temperature, and enzymatic kinetic parameters of TwUGT3 were determined.

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Section: Discussionmentioning

confidence: 99%

Investigating the Catalytic Activity of Glycosyltransferase on Quercetin from Tripterygium wilfordii

Gao

Lü

et al. 2020

ACS Omega

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“…So far 14 classes of flavonoids have been identified; they vary based on the chemical nature and position of substituents on the different rings [56] , [57] . The antimicrobial activity of flavonoids against an array of bacterial [58] , [59] , [60] and fungal pathogens [61] is resultant of their action on the microbial cell membranes [62] . Increase in permeability of membrane and disruption is observed due to their interaction with membrane proteins present on bacterial cell wall.…”

Section: Exploring Botanicals As An Alternativementioning

confidence: 99%

Development of botanicals to combat antibiotic resistance

Gupta

Birdi

2017

Journal of Ayurveda and Integrative Medicine

275

159

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The discovery of antibiotics in the previous century lead to reduction in mortality and morbidity due to infectious diseases but their inappropriate and irrational use has resulted in emergence of resistant microbial populations. Alteration of target sites, active efflux of drugs and enzymatic degradations are the strategies employed by the pathogenic bacteria to develop intrinsic resistance to antibiotics. This has led to an increased interest in medicinal plants since 25–50% of current pharmaceuticals are plant derived. Crude extracts of medicinal plants could serve as an alternate source of resistance modifying agents owing to the wide variety of secondary metabolites. These metabolites (alkaloids, tannins, polyphenols etc.) could act as potentials for antimicrobials and resistance modifiers. Plant extracts have the ability to bind to protein domains leading to modification or inhibition protein–protein interactions. This enables the herbals to also present themselves as effective modulators of host related cellular processes viz immune response, mitosis, apoptosis and signal transduction. Thus they may exert their activity not only by killing the microorganism but by affecting key events in the pathogenic process, thereby, the bacteria, fungi and viruses may have a reduced ability to develop resistance to botanicals. The article is meant to stimulate research wherein the cidal activity of the extract is not the only parameter considered but other mechanism of action by which plants can combat drug resistant microbes are investigated. The present article emphasizes on mechanisms involved in countering multi drug resistance.

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“…They facilitate pollination by acting as chemoattractants for insects, modulate plant physiology by signaling to beneficial microbiota in rhizosphere, and protect plants against predation due to their antimicrobial nature [36]. The marked antimicrobial property of flavonoids against a variety of bacterial [37–39] and fungal pathogens [40] is mediated by their action on the microbial cell membranes [41]. They interact with membrane proteins present on bacterial cell wall leading to increased membrane permeability and disruption.…”

Section: Plant-derived Antimicrobialsmentioning

confidence: 99%

Combating Pathogenic Microorganisms Using Plant-Derived Antimicrobials: A Minireview of the Mechanistic Basis

Upadhyay

Upadhyaya

Johny

et al. 2014

BioMed Research International

160

114

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The emergence of antibiotic resistance in pathogenic bacteria has led to renewed interest in exploring the potential of plant-derived antimicrobials (PDAs) as an alternative therapeutic strategy to combat microbial infections. Historically, plant extracts have been used as a safe, effective, and natural remedy for ailments and diseases in traditional medicine. Extensive research in the last two decades has identified a plethora of PDAs with a wide spectrum of activity against a variety of fungal and bacterial pathogens causing infections in humans and animals. Active components of many plant extracts have been characterized and are commercially available; however, research delineating the mechanistic basis of their antimicrobial action is scanty. This review highlights the potential of various plant-derived compounds to control pathogenic bacteria, especially the diverse effects exerted by plant compounds on various virulence factors that are critical for pathogenicity inside the host. In addition, the potential effect of PDAs on gut microbiota is discussed.

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Two Prenylated and C-Methylated Flavonoids fromTripterygium wilfordii

Cited by 18 publications

References 16 publications

Investigating the Catalytic Activity of Glycosyltransferase on Quercetin from Tripterygium wilfordii

Investigating the Catalytic Activity of Glycosyltransferase on Quercetin from Tripterygium wilfordii

Development of botanicals to combat antibiotic resistance

Combating Pathogenic Microorganisms Using Plant-Derived Antimicrobials: A Minireview of the Mechanistic Basis

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