Two populations of glial cells from fish optic nerve/tract with distinct electrophysiological properties

Abstract

Summary.The electrophysiological properties of the two major glial cell types in cultures from the regenerating goldfish optic nerve/tract were studied with patch-clamp techniques. Spindle-shaped cells express myelin proteins. These oligodendrocyte-like cells possess outwardly rectifying currents, do not show glutamate activated currents and are rarely electrically coupled to neighboring cells. Cells of epitheloid morphology probably represent astrocytes. They are GFAP-positive and do not exhibit myelin proteins. These cells have glutamate activated currents, display a linear current to voltage relationship and are extensively electrically coupled thus displaying properties similar to mammalian astrocytes.

Fish optic nerve oligodendrocytes support axonal regeneration of fish and mammalian retinal ganglion cells

“…The other group consists of compact cells that are GFAP positive but 0 4 negative. These cells represent fish astrocytes (Hoppe et al, 1991). Astrocytes usually occur in separate clusters.…”

“…Preliminary reports of these results were published as abstracts (Bastmeyer et al, 1991b) and in a brief review (Stuermer, 1991).…”

The isolation and culture of microglia-like cells from the goldfish brain

“…The procedure that we describe here differs in most respects from these earlier protocols and it is interesting that, with the exception of Sivron et al (1992), none of these papers reported the presence of microglial cells in their cultures. Most workers used L15 medium (Anderson et al, 1987;Bastmeyer et al, 1990;Gould et al, 1995;Sivron et al, 1992) although both Ham's F12 (Bastmeyer et al, 1993;Hoppe et al, 1990) and Dulbecco's modification of Eagle's medium (DMEM) (Jeserich and Rauen, 1990;Tocher and Wilson, 1990) have been used as well. In our cultures, we found that cells did not grow well in L15 and that we could not effectively buffer DMEM against pH changes in air.…”

“…Consequently, we moved to explant culture, a method that does not require enzyme treatment. This method has been used successfully to grow goldfish macroglia in culture (Bastmeyer et al, 1989;Hoppe et al, 1990). Brains were minced into small (0.5-1.0 m 2 ) fragments and plated into uncoated 24 well plates.…”

Estudio Morfométrico del Nervio Óptico de Tiburoncito (Ariopsis seemanni)

“…Los segundos tienen varias funciones, una está relacionada con la formación de gliales limitantes perivasculares y subpiales, creando agrupaciones de fibras nerviosas. También participan en procesos perinodales, permitiendo contactos estrechos con la membrana del axón en los nodos de Ranvier (Bolton & Butt, 2005;Butt et al, 2004;Maggs & Scholes, 1990) lo cual es importante en la regulación de las concentraciones de potasio K+, implicadas en el potencial de acción (Bolton & Butt, 2005;Hoppe et al, 1991).…”