Two-Ply Carbon Nanotube Fiber-Typed Enzymatic Biofuel Cell Implanted in Mice

Lee, Dong Yeop; Yun, Ji-Hyun; Park, Young Bin; Hyeon, Jae Sang; Jang, Yongwoo; Choi, Young-Bong; Kim, Hyug-Han; Kang, Tong Mook; Ovalle, Raquel; Baughman, Ray H.; Kim, Sung Min; Kee, Changdon; Kim, Seon Jeong

doi:10.1109/tnb.2020.2995143

Cited by 13 publications

(10 citation statements)

References 30 publications

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“…Indeed, our findings show that EBFCs have excellent potential to create self-powered devices for wearable sensor and medical implant applications related to bioelectrical stimulation of cells [3,23,62]. This potential is further supported by past animal studies showing that implanted EBFCs can be self-powered intracorporeally [62,63].…”

Section: Discussionsupporting

confidence: 72%

Modulation of Human Mesenchymal Stem Cells by Electrical Stimulation Using an Enzymatic Biofuel Cell

Jeon

Mun

et al. 2021

Catalysts

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Enzymatic biofuel cells (EBFCs) have excellent potential as components in bioelectronic devices, especially as active biointerfaces to regulate stem cell behavior for regenerative medicine applications. However, it remains unclear to what extent EBFC-generated electrical stimulation can regulate the functional behavior of human adipose-derived mesenchymal stem cells (hAD-MSCs) at the morphological and gene expression levels. Herein, we investigated the effect of EBFC-generated electrical stimulation on hAD-MSC cell morphology and gene expression using next-generation RNA sequencing. We tested three different electrical currents, 127 ± 9, 248 ± 15, and 598 ± 75 nA/cm2, in mesenchymal stem cells. We performed transcriptome profiling to analyze the impact of EBFC-derived electrical current on gene expression using next generation sequencing (NGS). We also observed changes in cytoskeleton arrangement and analyzed gene expression that depends on the electrical stimulation. The electrical stimulation of EBFC changes cell morphology through cytoskeleton re-arrangement. In particular, the results of whole transcriptome NGS showed that specific gene clusters were up- or down-regulated depending on the magnitude of applied electrical current of EBFC. In conclusion, this study demonstrates that EBFC-generated electrical stimulation can influence the morphological and gene expression properties of stem cells; such capabilities can be useful for regenerative medicine applications such as bioelectronic devices.

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Section: Discussionsupporting

confidence: 72%

Modulation of Human Mesenchymal Stem Cells by Electrical Stimulation Using an Enzymatic Biofuel Cell

Jeon

Mun

et al. 2021

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“…Indeed, our ndings show that EBFCs have excellent potential to create self-powered devices for wearable sensor and medical implant applications related to bioelectrical stimulation of cells [1,26,65]. This potential is further supported by past animal studies showing that implanted EBFCs can be self-powered intracorporeally [65,66].…”

Section: Discussionsupporting

confidence: 57%

Modulation of human mesenchymal stem cells by electrical stimulation using an enzymatic biofuel cell

Jeon

Mun

et al. 2020

Preprint

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Background Enzymatic biofuel cells (EBFCs) have excellent potential as components in wearable sensors and bioelectronic devices, especially as active biointerfaces to regulate stem cell behavior for regenerative medicine applications. However, it remains unclear to what extent EBFC-generated electrical stimulation can regulate the functional behavior of human adipose-derived mesenchymal stem cells (hAD-MSCs) at the morphological and gene expression levels. Herein, we investigated the effect of EBFC-generated electrical stimulation on hAD-MSC cell morphology and gene expression using next-generation RNA sequencing. Materials We tested three different electrical currents,127 ± 9, 248 ± 15, and 598 ± 75 nA/cm2, in mesenchymal stem cells. We performed transcriptome profiling to analyze the impact of EBFC-derived electrical current on gene expression using next generation sequencing (NGS). Also we observed changes in cytoskeleton arrangement and analyzed gene expression depends on the electrical stimulation. Results The electrical stimulation of EBFC changes cell morphology through cytoskeleton re-arrangement. In particular, the results of whole transcriptome NGS showed that specific gene clusters were up- or down-regulated depending on the magnitude of applied electrical current of EBFC. Conclusion Taken together, the findings in this study demonstrate that EBFC-generated electrical stimulation can influence the morphological and gene expression properties of stem cells and such capabilities can be useful for regenerative medicine applications related to wearable sensors and devices.

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“…A spinnable MWNT sheets that drawn from MWNT forest was prepared by stacking three-layer sheets with a length of 7.5 cm on glass. The MWNT sheets were coated with 8 wt% poly(3,4ethylenedioxythiophene (PEDOT) by vapor phase polymerization [32]. Each PEDOT coated MWNT sheets was fully immersed in the glucose oxidase and bilirubin oxidase (BOD) solution (150 mg/ml) for overnight respectively.…”

Section: Preparation Of Mediator-free Enzymatic Biofuel Cellsmentioning

confidence: 99%

Cytochrome c-Mediated Dual Functions for Real-Time ROS Monitoring and Sustainable Energy Supply in Implantable Device

Park,

Lee,

et al. 2024

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Smart stents integrate embedded sensors and advanced technology, providing a real-time diagnostic feedback, particularly for detection of thrombotic events. A continuous monitoring reactive oxygen species (ROS) in blood vessels is crucial for cardiovascular disease. The provision of a continuous power supply to sensors integrated within blood vessels is challenging. This study introduces a novel device that combines a sensor and supercapacitor, functioning as a ROS sensor and enabling continuous charging and discharging within blood vessels. This device employs thread-shaped electrodes integrated with cytochrome c and carbon nanotubes (Cyt.c/CNT). The Cyt.c/CNT electrode exhibits a high specificity to ROS with an excellent sensitivity (49.02 µAµM− 1cm− 2), as a real-time biosensor for monitoring of cellular ROS levels in living cells. In addition, it exhibited an energy storage performance of 257.95 mF/cm2 as a supercapacitor and maintained a stable performance during 10,000 repeated cycles in various biofluids. Notably, the integration of the Cyt.c/CNT electrode with an enzymatic biofuel cell enables continuous charging and discharging in a biofluid, making it a promising system for in-vivo applications such as smart stents. This study presents the potential of the biomaterial Cyt.c for ROS sensing as well as its potential as an energy storage system, showing new possibilities for implantable devices for cardiovascular diseases.

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Two-Ply Carbon Nanotube Fiber-Typed Enzymatic Biofuel Cell Implanted in Mice

Cited by 13 publications

References 30 publications

Modulation of Human Mesenchymal Stem Cells by Electrical Stimulation Using an Enzymatic Biofuel Cell

Modulation of Human Mesenchymal Stem Cells by Electrical Stimulation Using an Enzymatic Biofuel Cell

Modulation of human mesenchymal stem cells by electrical stimulation using an enzymatic biofuel cell

Cytochrome c-Mediated Dual Functions for Real-Time ROS Monitoring and Sustainable Energy Supply in Implantable Device

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