Two-photon spectroscopic properties of a new chlorin derivative photosensitizer

Zhao, Peide; Chen, P.; Tang, Guiqian; Zhang, G.L.; Chen, W.J.

doi:10.1016/j.cplett.2004.03.136

Cited by 23 publications

(13 citation statements)

References 11 publications

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“…The ns nature of the excited state lifetime was also found for Ce6 in a NaCl solution. 68 Certainly, the need of further single-molecule experimental and theoretical studies attempting to reproduce more reliably the required environment (depending on the type of application), constitute a recently proposed technological challenge. 79…”

Section: Emission Pathwaysmentioning

confidence: 99%

Photophysics of chlorin e6: from one- and two-photon absorption to fluorescence and phosphorescence

2017

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Section: Emission Pathwaysmentioning

confidence: 99%

Photophysics of chlorin e6: from one- and two-photon absorption to fluorescence and phosphorescence

2017

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“…According to the molecular structure of CDP and our former reports of the other derivates of this compound [14,19] , the considerable large value of TPA cross Figure 12 The nonlinear transmittance of the sample at the concentration C 0 = 2.28×10 3 mol/L. section should come from its molecular conjugate structure of the same rigid plane frame.…”

Section: Figure 11mentioning

confidence: 84%

“…They found that the sample molecule had a high quantum yield of singlet oxygen under one-photon excitation. We once reported concisely two-photon spectroscopic properties of another derivative of this kind compound [14] . Some behaviors of this molecule have not been studied and discussed carefully yet under two-photon excitation.…”

Section: Introductionmentioning

confidence: 99%

Two-photon spectroscopic behaviors and photodynamic effect on the BEL-7402 cancer cells of the new chlorophyll photosensitizer

Zhao

Chen

Tang

et al. 2008

Sci. China Ser. B-Chem.

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The spectroscopic properties of a new chlorophyll derivate photosensitizer (CDP) are studied under the excitation wavelengths at 800 and 400 nm using femtosecond pulses from a Ti: sapphire laser. The damaging effect of CDP on the BEL-7402 cancer cells is also investigated upon two-photon illumination at 800 nm. The normalized fluorescence spectra of CDP in tetrahydrofuran (THF) show that two-photon and one-photon spectra have the same distributions and the same emission bands (675 nm). The lifetimes of two-and one-photon induced fluorescence of this molecule are of the order of 5.0 ns. By comparing the data it is shown that there is some difference between the two lifetimes, but the difference is less than one nanosecond. The two-photon absorption cross section of the molecule is also measured at 800 nm and estimated as about V ƍ 2 § 31.5×10 50 cm 4 ·s·photon 1 . The results of two-photon photodynamic therapy (TPPDT) tests show that CDP can kill all of the tested cancer cells according to the usual Eosine assessment. Our results indicate that the two-photon-induced photophysical, photochemical and photosensitizing processes of CDP may be basically similar to those of one-photon excitation. These behaviors of the sample suggest that one may find other possible methods to estimate some photosensitizers' effects in details such as their distribution in cells and the reactive targets of the sub-cellular parts of some tumor cells via two-photon excitation techniques. photosensitizer, chlorophyll derivate, two-photon fluorescence, two-photon absorption cross-section, TPPDT, BEL-7402 cell

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“…Some researchers [5][6][7][8] have already investigated the optical characteristics and the Photodynamic effects of some CPD s . However, the details about the interaction of the CPD s with tumor DNA have not yet been reported so far.…”

Section: ⅰ Introductionmentioning

confidence: 99%

The interaction of Chlorophyll derivative photosensitizer CPD3 with mouse lung cancer DNA

Xiang

Lin

Wang

et al. 2006

2006 International Symposium on Biophotonics, Nanophotonics and Metamaterials

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CPD 3 is a new kind of photosensitizer (PS) with promising applications in photodynamic therapy (PDT) for anticancer treatment. The binding properties of CPD 3 to mouse lung cancer cell DNA have been investigated by using absorption spectroscopy. In neutral CPD 3 -DNA mix solution in NS, the peak wavelengths of absorption bands of CPD 3 (B and Q band) do not change basically but the intensities of them do decrease with increase of the concentration of the DNA, in addition, some new red-shifted absorption bands appear in the spectra and become stronger with increasing the concentration of the DNA and with prolonging incubation period of the sample solution. In basic mix solution (pH=8.0), the situation above is not observed and absorption bands of CPD 3 are not yet changed in the peak position and intensity even though the concentration of DNA in the solution is high enough to 1.75×10 -5 mg/l. The study shows that some free CPD 3 molecules bind to DNA to form a "complex" through intensive groove and hydrogen bonding interaction between them in a proper time period. However, the electrostatic interaction between CPD 3 and the DNA is not important for formation of the "complex". The intercalative binding model included groove, hydrogen bonding, and spatial obstacle interaction would be preferable. The photobleaching (PB) properties of the CPD 3 solution with and without the DNA are investigated under irradiating with LD at 674nm and 20mW. For the former, PB lifetime of CPD 3 is 49.3 seconds, for the latter, 96.2 seconds. Both the decay curves obey single exponential drop rule exactly. The results show that the PB of CPD 3 is a single excited state reaction; the intercalative binding between CPD 3 and DNA can increase the rate constant for PB reaction. The electrophoresis graph of the bleached solution indicates that the DNA is destroyed basically. The study indicates the CPD 3 is an efficient and stable PS for PDT. Ⅰ. INTRODUCTIONIn resent years, the study of the interactions of various small organic molecules with DNA has been of great interest because their importance in understanding the effects of the drug on DNA and the consequent designs of new efficient drugs targeted to DNA [1][2][3][4] . As well known, there are mainly three interaction models about of organic molecules and the DNA double helix: electrostatic binding, groove (or surface) binding and intercalative binding. Electrostatic binding interactions between cationic species and the negatively charged DNA phosphate backbone occur usually along the exterior of the helix. Groove binding generally involves direct hydrogen bonding, Van der Waals interactions with the nucleic acid bases in the deep minor groove or the wide shallow major groove of the DNA helix. The intercalative binding and groove binding are related to the grooves in the DNA double helix, but the electrostatic binding can take place out of the groove.Chlorophyll derivatives (CPD s ) are a new class of potential photosensitizer (PS) in photodynamic therapy (PDT) of the tumors. Some researc...

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Two-photon spectroscopic properties of a new chlorin derivative photosensitizer

Cited by 23 publications

References 11 publications

Photophysics of chlorin e6: from one- and two-photon absorption to fluorescence and phosphorescence

Photophysics of chlorin e6: from one- and two-photon absorption to fluorescence and phosphorescence

Two-photon spectroscopic behaviors and photodynamic effect on the BEL-7402 cancer cells of the new chlorophyll photosensitizer

The interaction of Chlorophyll derivative photosensitizer CPD3 with mouse lung cancer DNA

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