Two-photon near- and far-field fluorescence microscopy with continuous-wave excitation

Hell, Stefan W.; Booth, Martin J.; Wilms, Stefan; Schnetter, Christoph M.; Kirsch, Achim K.; Arndt‐Jovin, Donna J.; Jovin, Thomas M.

doi:10.1364/ol.23.001238

Cited by 80 publications

(60 citation statements)

References 16 publications

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“…If the distance within probe and object is made less than l, the size of the probe's light spot on the sample becomes less than the diffraction limit, as was demonstrated by [133], and resolution in near-field appears to be much better than with the characteristic height h ) l (far-field). Practically, SNOM resolution reaches 50 nm [134]. Theoretically, this limit may be decreased to less than 1 nm.…”

Section: Potentialities Of Confocal and Near-field Microscopiesmentioning

confidence: 97%

Nanotechnologies in proteomics

Ivanov

Govorun

Быков³

et al. 2006

Proteomics

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Progress in proteomic researches is largely determined by development and implementation of new methods for the revelation and identification of proteins in biological material in a wide concentration range (from 10 23 M to single molecules). The most perspective approaches to address this problem involve (i) nanotechnological physicochemical procedures for the separation of multicomponent protein mixtures; among these of particular interest are biospecific nanotechnological procedures for selection of proteins from multicomponent protein mixtures with their subsequent concentration on solid support; (ii) identification and counting of single molecules by use of molecular detectors. The prototypes of biospecific nanotechnological procedures, based on the capture of ligand biomolecules by biomolecules of immobilized ligate and the concentration of the captured ligands on appropriate surfaces, are well known; these are affinity chromatography, magnetic biobeads technology, different biosensor methods, etc. Here, we review the most promising nanotechnological approaches for selection of proteins and kinetic characterization of their complexes based on these biospecific methods with subsequent MS/MS identification of proteins and protein complexes. Two major groups of methods for the analysis and identification of individual molecules and their complexes by use of molecular detectors will be reviewed: scanning probe microscopy (SPM) (including atomic-force microscopy) and cryomassdetector technology.

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Section: Potentialities Of Confocal and Near-field Microscopiesmentioning

confidence: 97%

Nanotechnologies in proteomics

Ivanov

Govorun

Быков³

et al. 2006

Proteomics

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“…The field is covered from optomagnetics up to Raman spectroscopy or two-color fluorescence imaging [30][31][32]. Commercial SNOM setups show limitations for the space and the detector one can use [3] and the number of light sources is limited.…”

Section: Scanning Near-field Microscopymentioning

confidence: 99%

Comparative scanning near-field optical microscopy studies of plasmonic nanoparticle concepts

Andrae

Fumagalli

Schmid

2014

Optical Micro- And Nanometrology V

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We use scanning near-field optical microscopy (SNOM) to characterize different plasmonic-nanoparticle situations with high spatial and spectral resolution in this comparative study. The near-field enhancement is measured with an aperture probe (Al coated glass fiber) and two CCD spectrometers for simultaneous detection of reflection and transmission. The images of transmission and reflection show a correlation to the topography. We present a new way to access the relative absorption and discuss the results with consideration of artifact influences. Near-field enhancements are deeper understood by imaging isolated particles. This near field will be compared to measurements of random-particle distributions. Therefore, we will show normalized reflection and transmission images of random structures that lay the foundation for an absolute interpretation of near-field images. The normalization considers both the far-field UV/VIS results and a reference image of the substrate. The near-field reflection of nanoparticle arrays shows an enhancement of 25 %. In view of specific applications, particle distributions implemented in two ways: as far-field scatters and as near field enhancing objects.

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“…Nowadays, a variety of different implementations of SNOM exists. Starting from the visionary work of Synge in 1929 [25] in which the basic idea of apertured SNOM was presented, the concept of a microscopy technique based on the local interaction of light mediated by a nanoprobe has been extended including, but not limited at, Scattering-type SNOM (s-SNOM) [26], collection mode SNOM [27], illumination-mode SNOM [28], PSTM (Photon Scanning Tunnelling Microscopy) [29], coupled to several detection methods ranging from interferometric homodyne [30], heterodyne [31,32], to spectrometric (fluorescence [33,34] and Raman [35]). A comprehensive description of SNOM-related techniques is outside the scope of this work.…”

Section: Techniques For the Characterization Of Surfaces At The Nanosmentioning

confidence: 99%

Biomimetic Tailoring of the Surface Properties of Polymers at the Nanoscale: Medical Applications

Chiono

Descrovi

Sartori

et al. 2010

Scanning Probe Microscopy in Nanoscience and Nanotechnology 2

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New-generation biomaterials are information-rich and incorporate biologically active components derived from Nature. The chapter reviews current approaches for the realization of biomimetic coatings for tissue engineering applications, via functionalization with bioactive molecules such as native long chains of extracellular matrix (ECM) proteins as well as short peptide sequences derived from intact ECM proteins. Biomimetic materials provide cues for cell-matrix interactions, favouring cell adhesion, proliferation, spreading, and differentiation. The recently developed scanning probe techniques for optical and spectroscopic characterization of surfaces are also described, providing advanced topographical imaging and sensitive force measurements. The spectroscopic imaging of surfaces at the nanoscale provides insight into the function and structure of biomimetic molecules and represents a tool for tailoring the surface design.

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Two-photon near- and far-field fluorescence microscopy with continuous-wave excitation

Cited by 80 publications

References 16 publications

Nanotechnologies in proteomics

Nanotechnologies in proteomics

Comparative scanning near-field optical microscopy studies of plasmonic nanoparticle concepts

Biomimetic Tailoring of the Surface Properties of Polymers at the Nanoscale: Medical Applications

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