Two-Photon Fluorescent Turn-On Probe for Lipid Rafts in Live Cell and Tissue

Kim, Hwan Myung; Jeong, Byeong Ha; Hyon, Ju-Yong; An, Myoung Jin; Seo, Mun Sik; Hong, Jin Hee; Lee, Kyoung J.; Kim, Chul Hoon; Joo, Taiha; Hong, Seok-Cheol; Cho, Bong Rae

doi:10.1021/ja711391f

Cited by 126 publications

(107 citation statements)

References 12 publications

Supporting

Mentioning

100

Contrasting

Unclassified

Order By: Relevance

“…These probes were used to detect the membrane domains in model membranes, as well as in living cells, by two-photon microscopy (Parasassi et al 1997;Bagatolli and Gratton 1999, 2000a,b;Dietrich et al 2001;Bagatolli 2003;Bagatolli et al 2003;Kaiser et al 2009). The order of different membrane systems was investigated (Gasecka et al 2009), and new probes to visualize the membrane order were tested by two-photon microscopy (Jin et al 2006;Kim et al 2008;Klymchenko et al 2009). …”

Section: Two-photon Microscopymentioning

confidence: 99%

Fluorescence Techniques to Study Lipid Dynamics

Sezgin¹,

Schwille²

2011

Cold Spring Harbor Perspectives in Biology

View full text Add to dashboard Cite

Biological research has always tremendously benefited from the development of key methodology. In fact, it was the advent of microscopy that shaped our understanding of cells as the fundamental units of life. Microscopic techniques are still central to the elucidation of biological units and processes, but equally important are methods that allow access to the dimension of time, to investigate the dynamics of molecular functions and interactions. Here, fluorescence spectroscopy with its sensitivity to access the single-molecule level, and its large temporal resolution, has been opening up fully new perspectives for cell biology. Here we summarize the key fluorescent techniques used to study cellular dynamics, with the focus on lipid and membrane systems.T o elucidate cellular processes in their native dynamic environment has been one of the main issues in cell biology over the past decades. The lack of appropriate techniques has long been the main limiting step for the research on dynamic systems, because it was impossible to acquire real time information with the well-known biochemical techniques. The key challenge in dynamically observing biological systems is to combine the ability to resolve moderate to very low concentrations of molecules-because they are simply limited in living cells-on relevant timescales. Relevant timescales in cell biology can be minutes and hours, on a systemic level of cell metabolism, down to the microsecond and even nanosecond regime in which molecular and intramolecular rearrangements take place. With respect to lipidic systems, relevant dynamics range from the local movements of lipids by diffusion to the mechanical transformations of whole membranes, spanning several orders of magnitude in time to be covered. Like for other cellular processes, the investigation of lipids and membranes also in general benefited greatly from the introduction of fluorescence microscopy and spectroscopy to biology. After the 1960s, great technological inventions based on the phenomenon of fluorescence were made, such as confocal microscopy, fluorescence recovery after photobleaching (FRAP), fluorescence correlation spectroscopy (FCS), Förster resonance energy transfer (FRET), total internal reflection fluorescence (TIRF), and two-photon microscopy, that not only revolutionized imaging but also yielded access to dynamics on previously inaccessible timescales. Another very big step was certainly taken after the introduction of fluorescent proteins, which again accelerated the use of these techniques in living cells and organisms. Nowadays, the technical advancements of fluorescence-based methods allow us to explore systems as small as single molecules with temporal resolution down to the nanoseconds regime. Lately, even the resolution limit of optical microscopy, for a long time being one of the fundamental barriers in elucidating cellular processes, has been overcome by smart applications of the phenomenon of fluorescence.This article aims at giving a short overview on mainly fluorescence-based metho...

show abstract

Section: Two-photon Microscopymentioning

confidence: 99%

Fluorescence Techniques to Study Lipid Dynamics

Sezgin¹,

Schwille²

2011

Cold Spring Harbor Perspectives in Biology

View full text Add to dashboard Cite

show abstract

“…High throughput fiber optic imaging configurations combined with miniature gradient-index ͑GRIN͒ lens has led to the design of miniaturized two-photon microscopes 122,142 and microendoscopes. [143][144][145] These portable, lightweight microscopes show tremendous promise for biomedical research and noninvasive imaging-potentially eliminating the need for multiple biopsies when identifying and tracking cancerous cells.…”

Section: A Tpefmentioning

confidence: 99%

Invited Review Article: Imaging techniques for harmonic and multiphoton absorption fluorescence microscopy

Carriles

Schafer²,

Sheetz³

et al. 2009

Review of Scientific Instruments

152

125

View full text Add to dashboard Cite

We review the current state of multiphoton microscopy. In particular, the requirements and limitations associated with high-speed multiphoton imaging are considered. A description of the different scanning technologies such as line scan, multifoci approaches, multidepth microscopy, and novel detection techniques is given. The main nonlinear optical contrast mechanisms employed in microscopy are reviewed, namely, multiphoton excitation fluorescence, second harmonic generation, and third harmonic generation. Techniques for optimizing these nonlinear mechanisms through a careful measurement of the spatial and temporal characteristics of the focal volume are discussed, and a brief summary of photobleaching effects is provided. Finally, we consider three new applications of multiphoton microscopy: nonlinear imaging in microfluidics as applied to chemical analysis and the use of two-photon absorption and self-phase modulation as contrast mechanisms applied to imaging problems in the medical sciences.

show abstract

“…Although a small number of TPF probes for polarity were reported, and two among them were used for lipid rafts imaging, their applications can't be extended from polarity detection to viscosities and temperature. Furthermore, in them, small molecule-size always is accompanied by narrow solvatochromic range and small δ [1][2][3][4][5]. So they are not ideal candidates for TP solvatochromic probes.…”

Section: Introductionmentioning

confidence: 99%

Dicyanostilbene-based Two-photon Thermo-solvatochromic Fluorescence Probes with Two-photon Triple Fluorescence

Huang¹,

Junle²

2017

Biomark J

View full text Add to dashboard Cite

The unusually sensitive solvatochromism of the two-photon fluorescence fluorophore of 2,5-dicyano-4-methyl-4'-dimethylaminostilbene (P1) whose emission maximum varies from 445 nm in cyclohexane to 641 nm in DMSO are described. P1 with remarkably large two-photon cross sections exhibits very strong polarity-, viscosity-, and temperature-dependence of fluorescence, and can be used to detect polarities, viscosities, and temperature. P1 presents a twophoton triple fluorescence.

show abstract

Two-Photon Fluorescent Turn-On Probe for Lipid Rafts in Live Cell and Tissue

Cited by 126 publications

References 12 publications

Fluorescence Techniques to Study Lipid Dynamics

Fluorescence Techniques to Study Lipid Dynamics

Invited Review Article: Imaging techniques for harmonic and multiphoton absorption fluorescence microscopy

Dicyanostilbene-based Two-photon Thermo-solvatochromic Fluorescence Probes with Two-photon Triple Fluorescence

Contact Info

Product

Resources

About