Two-photon fluorescence bioimaging with an all-semiconductor laser picosecond pulse source

Kuramoto, Masaru; Kitajima, N.; Guo, Haotian; Furushima, Y.; Ikeda, Masao; Yokoyama, Hiroyuki

doi:10.1364/ol.32.002726

Cited by 78 publications

(52 citation statements)

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“…Another trend towards practical TPM system is the development of compact and turn-key light sources. The emerging compact laser systems are based on semiconductor laser-diode (LD) systems [15,27,[51][52][53]59,60] , optical fiber laser systems [57,58,61] , and even chip-size devices [62] . LD has been used as a reliable, low-cost, highperformance light source in scientific research, optical communication, andinformation technologies.…”

Section: Laser Systemmentioning

confidence: 99%

“…Photo of 500-MHz repetition rate, 2.7-ps pulse width, and 1-µm semiconductor laser oscillator [15] . (c) TPM of convoluted tubules in mouse kidney tissues stained with Alexa Fluor 488 using all-semiconductor light source [53] . (d) TPM of brain Purkinje cells express GFP using 1 030-nm semiconductor light source [15] .…”

Section: Laser Systemmentioning

confidence: 99%

“…Kilowatt peak-power picosecond laser pulses at 775, 800, and 1 030 nm have been generated for TPF imaging of mouse kidney tissues and mouse brain neurons expressing GFP (Figs. 2(c) and (d)) [15,51,53] .…”

Section: -2mentioning

confidence: 99%

See 2 more Smart Citations

Recent advances in two-photon imaging: technology developments and biomedical applications

Chen¹,

Guo²,

Gong³

et al. 2013

中国光学快报

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In the past two decades, two-photon microscopy (TPM) transforms biomedicalresearch, allowing nondestructive high-resolution fluorescent molecular imaging and label-free imaging in vivo and in real time.Here we review the recent advances of TPM technologyincluding novel laser sources, new image acquisition paradiams, and microendoscopicimaging systems. Then, we survey the capabilities of TPM imagingof biological relevant molecules such as nicotinamideadenine dinucleotide (NADH), flavin adenine dinucleotide (FAD),and reactive oxygen species (ROS). Biomedical applications of TPM in neuroscience and cancer detection are demonstrated.

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Section: Laser Systemmentioning

confidence: 99%

Section: Laser Systemmentioning

confidence: 99%

See 1 more Smart Citation

Recent advances in two-photon imaging: technology developments and biomedical applications

Chen¹,

Guo²,

Gong³

et al. 2013

中国光学快报

View full text Add to dashboard Cite

show abstract

“…It was demonstrated that pulse durations as short as a few picoseconds are required in order to get two photon excitation and second harmonic generation images from biological samples [5][6]. Picosecond pulses can be also used as a seed source for a master oscillator power amplifier (MOPA) for industrial applications like micro-machining and ablation [11].…”

Section: Laser Characteristics Without Sweepingmentioning

confidence: 99%

“…One possible weak side of picosecond pulses versus femtosecond pulses would be towards other nonlinear microscopy applications like second harmonic generation (SHG) microscopy and two photon excitation (TPE) microscopy. However it has been also demonstrated that a several picoseconds pulse width is efficient enough to get high contrast image in second harmonic generation (SHG) microscopy and two-photon excitation (TPE) microscopy [5][6].…”

Section: Introductionmentioning

confidence: 99%

Picosecond programmable laser sweeping over 50 mega-wavelengths per second

et al. 2011

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We report here the successful realization of 25 millions wavelengths per second using an SOA based PL around 1565 nm at a 75 MHz repetition rate. The laser is simply composed of an SOA, a CFBG (10 ps/nm) with a 100 nm bandwidth, an optical circulator, an EOM (intensity modulator), and an output coupler (20%). Pulse duration is around 45 ps and OSNR of the pulse is around 35 dB at 1565 nm without sweeping. Tunable dispersion compensating module (TDCM) was used to compress the chirped pulse output and 10 ps pulse duration was obtained at 1548 nm. Finally 25 megawavelengths per second was realized with under 3 pulses per wavelength and 1024 discrete wavelengths. Linear k-space sweeping function was enabled in the swept-source OCT (SS-OCT) system through graphical user interface (GUI).

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Ultra‐Short‐Pulse QD Edge‐Emitting Lasers

Breuer

Syvridis

Рафаилов

2014

The Physics and Engineering of Compact Quantum Dot‐based Lasers for Biophotonics

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IntroductionHigh-power, electrically pumped edge-emitting monolithic semiconductor lasers based on InAs/GaAs quantum dots (QDs) offer significant advantages including ultra-short pulse generation due to ultrafast gain and absorption recovery [1, 2] and emission energies in the near-infrared spectral range [3]. Owing to their inherent inhomogeneous QD size distribution, a broadening of gain and absorption profile is realized, leading to broadband wavelengths emission. This inhomogeneous broadening together with ultrafast carrier dynamics is particularly advantageous for ultrafast applications including the generation, propagation, and amplification of subpicosecond optical pulses with high pulse peak power [4] and the generation of spectrally tunable emission [5,6]. The reduced density of states allows for the exploitation of QD materials in harnessing spectral versatility in novel dual-state emission. The spectral coverage of these QD lasers perfectly matches a wide range of relevant biophotonic applications including nonlinear excitation imaging techniques. The unique combination of efficient light generation, ultrafast carrier dynamics, and broadband gain bandwidth can deliver exclusive advantages in ultrafast microelectronic scale components, thereby stimulating major advances in ultrafast science and technology.In order to enable initial device design and optimization of novel pulse generating QD lasers and amplifiers, a new and efficient theoretical framework is presented as briefly introduced in Section 2.2. This framework takes into account specifically fundamental aspects associated with QD gain materials and allows for modeling of both continuous-wave (CW) emission and ultra-short pulse generation via passive mode-locking (PML) and subsequent amplification. The obtained understanding is applied to technologically realize and optimize QD laser and amplifier structures with particularly broad gain bandwidth enabling spectral versatility through broadband tunability of CW-emission generated in the visible wavelength range by second-harmonic-generation (SHG). This broad wavelength range is accessible only by the QD features as presented in Section 2.3. Novel functionalities based on the broad gain bandwidth and sophisticated QD laser design are explored inThe Physics and Engineering of Compact Quantum Dot-based Lasers for Biophotonics, First Edition. Edited by Edik U. Rafailov.

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Two-photon fluorescence bioimaging with an all-semiconductor laser picosecond pulse source

Cited by 78 publications

References 0 publications

Recent advances in two-photon imaging: technology developments and biomedical applications

Recent advances in two-photon imaging: technology developments and biomedical applications

Picosecond programmable laser sweeping over 50 mega-wavelengths per second

Ultra‐Short‐Pulse QD Edge‐Emitting Lasers

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