Two-photon and second harmonic generation microscopy of porcine eye: implications to conductive keratoplasty

Abstract

Two-photon autofluorescence and second harmonic generation (SHG) microscopy are useful imaging techniques for studying tissue components. In this work, we apply this imaging modality to study porcine eye. In particular, we use SHG microscopy to investigate the structural changes to excised porcine corneas and found that this technique is useful for studying its structural changes under thermal treatment.