Two-photon absorption standards in the 550-1600 nm excitation wavelength range

Makarov, Nikolay S.; Drobizhev, Mikhail; Rebane, Aleksander

doi:10.1364/oe.16.004029

Cited by 797 publications

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“…As shown in Figure 2C, the two‐photon excitation spectrum of AN in water exhibited a broadband from 690 to 830 nm and the maximum is around 740 nm, which is double of the optimum one‐photon excitation wavelength (370 nm). The AN excitation cross section ( δφ ) at 740 nm is around 25 GM, which is in agreement to the literatures 9, 10. In other words, the intrinsic brightness of the AN is sufficient for two‐photon microscopic applications.…”

supporting

confidence: 91%

A Two‐Photon Excitation Based Fluorogenic Probe for Sialome Imaging in Living Systems

et al. 2015

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supporting

confidence: 91%

A Two‐Photon Excitation Based Fluorogenic Probe for Sialome Imaging in Living Systems

et al. 2015

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“…The corresponding cross-sections for serotonin are shown for comparison and are approximately twofold higher than for propranolol despite the lower extinction coefficients for the former at 295 and 315 nm. For comparison, a second determination of σ 2 for propranolol was made with 2PE at 628 nm using 9-chloroanthracene as a standard with σ 2r = 0.20 GM at 630 nm [25]. Using again the method outlined by Mathai et al [24], a value of σ 2 for propranolol of 0.22 GM at 628 nm was obtained which is in excellent agreement with the value of 0.17 GM units obtained with tryptophan as the standard.…”

Section: Resultssupporting

confidence: 67%

Fluorescence Lifetime Imaging of Propranolol Uptake in Living Glial C6 Cells

Bisby

Botchway²,

Crisostomo³

et al. 2012

Journal of Spectroscopy

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Abstract. Uptake of the β-blocker drug propranolol by living glial C6 cells has been observed using fluorescence lifetime imaging with two-photon excitation at 630 nm. Both uptake and release of propranolol occur within minutes and are temperature dependent, being about 5 times faster at 37 • C than at 20 • C. The intracellular fluorescence lifetime of propranolol is generally shorter than the value of 9.8 ns determined in dilute neutral aqueous solution, and the difference is ascribed to concentration quenching. Within the cells, propranolol is accumulated within intracellular acidic vesicles and the cytoplasm but is excluded from the cell nucleus. On incubation of cells in medium containing 100 μM propranolol, the drug is accumulated to reach intracellular concentrations up to 10 mM in a process that is believed to be driven by protonation within acidic cellular compartments.

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“…16 The subscript R indicates the parameters of the fluorescent reference dye fluorescein with well-reviewed Φ F,R = 0.93 and 2P absorption cross-sections σ 2,R . 18,19 For determination of the actual concentrations, absorption measurements were performed at a Lambda 750 UV/VIS spectrometer (Perkin Elmer, Waltham, USA) in the range of 400-600 nm. Fluorescence quantum yields were determined absolutely with the C 9929 integration sphere system (Hamamatsu, Hamamatsu City, Japan).…”

Section: P Fluorescence Excitation Spectramentioning

confidence: 99%

ANG-2 for quantitative Na+ determination in living cells by time-resolved fluorescence microscopy

Roder

Hille

2014

Photochem Photobiol Sci

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U n i v e r s i t ä t P o t s d a mPostprints der Universität Potsdam Mathematisch-Naturwissenschaftliche Reihe ; 184 Phillip Roder and Carsten Hille* Sodium ions (Na + ) play an important role in a plethora of cellular processes, which are complex and partly still unexplored. For the investigation of these processes and quantification of intracellular Na + concentrations ([Na + ] i ), two-photon coupled fluorescence lifetime imaging microscopy (2P-FLIM) was performed in the salivary glands of the cockroach Periplaneta americana. For this, the novel Na + -sensitive fluorescent dye Asante NaTRIUM Green-2 (ANG-2) was evaluated, both in vitro and in situ. In this context, absorption coefficients, fluorescence quantum yields and 2P action cross-sections were determined for the first time. ANG-2 was 2P-excitable over a broad spectral range and displayed fluorescence in the visible spectral range. Although the fluorescence decay behaviour of ANG-2 was triexponential in vitro, its analysis indicates a Na + -sensitivity appropriate for recordings in living cells. The Na + -sensitivity was reduced in situ, but the biexponential fluorescence decay behaviour could be successfully analysed in terms of quantitative [Na + ] i recordings. Thus, physiological 2P-FLIM measurements revealed a dopamineinduced [Na + ] i rise in cockroach salivary gland cells, which was dependent on a Na + -K + -2Cl − cotransporter (NKCC) activity. It was concluded that ANG-2 is a promising new sodium indicator applicable for diverse biological systems.

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Two-photon absorption standards in the 550-1600 nm excitation wavelength range

Cited by 797 publications

References 0 publications

A Two‐Photon Excitation Based Fluorogenic Probe for Sialome Imaging in Living Systems

A Two‐Photon Excitation Based Fluorogenic Probe for Sialome Imaging in Living Systems

Fluorescence Lifetime Imaging of Propranolol Uptake in Living Glial C6 Cells

ANG-2 for quantitative Na+ determination in living cells by time-resolved fluorescence microscopy

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