Two-Photon 3D FIONA of Individual Quantum Dots in an Aqueous Environment

Zhang, Ruobing; Rothenberg, Eli; Fruhwirth, Gilbert O.; Simonson, Paul D.; Ye, Fangfu; Golding, Ido; Ng, Tony; Lopes, Ward; Selvin, Paul R.

doi:10.1021/nl201225r

Cited by 36 publications

(39 citation statements)

References 25 publications

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“…Their technique allowed an 80-fold increase of the imaging rate. 190 Myosin V rotation around its own axis during the movement could be shown by Ohmachi et al, who developed a single-molecule fluorescence polarization technique for 3D orientation measurements. Their method allowed an orientation accuracy of 101 at 33 ms temporal resolution and revealed a myosin V rotation of 901 during each step.…”

Section: Single-particle Trackingmentioning

confidence: 99%

Quantum dots: bright and versatile in vitro and in vivo fluorescence imaging biosensors

2015

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Semiconductor quantum dots (QDs) have become important fluorescent probes for in vitro and in vivo bioimaging research. Their nanoparticle surfaces for versatile bioconjugation, their adaptable photophysical properties for multiplexed detection, and their superior stability for longer investigation times are the main advantages of QDs compared to other fluorescence imaging agents. Here, we review the recent literature dealing with the design and application of QD-bioconjugates for advanced in vitro and in vivo imaging. After a short summary of QD preparation and their most important properties, different QD-based imaging applications will be discussed from the technological and the biological point of view, ranging from super-resolution microscopy and single-particle tracking over in vitro cell and tissue imaging to in vivo investigations. A substantial part of the review will focus on multifunctional applications, in which the QD fluorescence is combined with drug or gene delivery towards theranostic approaches or with complementary technologies for multimodal imaging. We also briefly discuss QD toxicity issues and give a short outlook on future directions of QD-based bioimaging.

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Section: Single-particle Trackingmentioning

confidence: 99%

Quantum dots: bright and versatile in vitro and in vivo fluorescence imaging biosensors

2015

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“…15). 254 2PE FIONA was first validated by tracking the steps of individual QD 655 -labeled myosin V motors on F-actin with nanometer accuracy (step size, 35.8 6 6.3 nm for 2PE and 35.4 6 7.0 nm for 1PE). Impressively, widefield illumination at 785 nm was used for the 2PE tracking and was uniquely enabled by the efficient 2PE of QDs.…”

Section: Bioanalysis and Bioimaging With Quantum Dotsmentioning

confidence: 99%

“…The 2PE-FIONA technique provided a five-fold enhancement in S/N compared with imaging with 1PE total internal reflection microscopy. 254 Another useful application for QDs in 2PE microscopy is in vivo imaging. Larson et al demonstrated in vivo 2PE imaging of mouse vasculature (i.e., angiography) in skin and adipose tissue by injection of amphiphilic polymercoated CdSe/ZnS QD 550 and 2PE at 880 nm.…”

Section: Bioanalysis and Bioimaging With Quantum Dotsmentioning

confidence: 99%

Quantum Dots in Bioanalysis: A Review of Applications across Various Platforms for Fluorescence Spectroscopy and Imaging

2013

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Semiconductor quantum dots (QDs) are brightly luminescent nanoparticles that have found numerous applications in bioanalysis and bioimaging. In this review, we highlight recent developments in these areas in the context of specific methods for fluorescence spectroscopy and imaging. Following a primer on the structure, properties, and biofunctionalization of QDs, we describe select examples of how QDs have been used in combination with steady-state or time-resolved spectroscopic techniques to develop a variety of assays, bioprobes, and biosensors that function via changes in QD photoluminescence intensity, polarization, or lifetime. Some special attention is paid to the use of Förster resonance energy transfer-type methods in bioanalysis, including those based on bioluminescence and chemiluminescence. Direct chemiluminescence, electrochemiluminescence, and charge transfer quenching are similarly discussed. We further describe the combination of QDs and flow cytometry, including traditional cellular analyses and spectrally encoded barcode-based assay technologies, before turning our attention to enhanced fluorescence techniques based on photonic crystals or plasmon coupling. Finally, we survey the use of QDs across different platforms for biological fluorescence imaging, including epifluorescence, confocal, and twophoton excitation microscopy; single particle tracking and fluorescence correlation spectroscopy; super-resolution imaging; near-field scanning optical microscopy; and fluorescence lifetime imaging microscopy. In each of the above-mentioned platforms, QDs provide the brightness needed for highly sensitive detection, the photostability needed for tracking dynamic processes, or the multiplexing capacity needed to elucidate complex systems. There is a clear synergy between advances in QD materials and spectroscopy and imaging techniques, as both must be applied in concert to achieve their full potential.

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“…These properties make the Lipo-UCNP an excellent probe for single molecule imaging. [11] Furthermore, the Lipo-UCNPs showed long-term stability in water and resistance to aggregation over an extended period of several months (Figure S4).…”

mentioning

confidence: 99%

Biomimetic Surface Engineering of Lanthanide‐Doped Upconversion Nanoparticles as Versatile Bioprobes

et al. 2012

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A general and versatile biomimetic approach to synthesize water dispersible and functionalizable upconverting nanoparticles (UCNPs) for selective imaging of live cancer cells is reported. The approach involves coating the surface of UCNPs with a monolayer of phospholipids containing different functional groups, allowing for conjugation of many molecules for a wide range of applications in fields such as bioinspired nanoassembly, biosensing, and bio-medicine.

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Two-Photon 3D FIONA of Individual Quantum Dots in an Aqueous Environment

Cited by 36 publications

References 25 publications

Quantum dots: bright and versatile in vitro and in vivo fluorescence imaging biosensors

Quantum dots: bright and versatile in vitro and in vivo fluorescence imaging biosensors

Quantum Dots in Bioanalysis: A Review of Applications across Various Platforms for Fluorescence Spectroscopy and Imaging

Biomimetic Surface Engineering of Lanthanide‐Doped Upconversion Nanoparticles as Versatile Bioprobes

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