Two-phase Endolymphatic Hydrops: A New Dynamic Guinea Pig Model

Dunnebier, Erwin A.; Segenhout, J. M.; Wit, H. P.; Albers, F. W. J.

doi:10.3109/00016489709117984

Cited by 57 publications

(49 citation statements)

References 19 publications

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“…However, abnormal OCT findings in EH, such as Reissner's membrane distension, were confirmed by comparison with pathologic analyses in previous studies, 9,16 and the abnormal findings of OCT images in this study were consistent with those of previous studies. Both 2-D and 3-D OCT images clearly showed the abnormal EH findings, indicating the potential of OCT as an effective tool for analyzing cochlear pathologies.…”

Section: Discussionsupporting

confidence: 82%

“…It is difficult to identify EH in patients with Meniere's disease because clinical examinations such as the auditory brainstem response (ABR) and caloric tests only provide functional information regarding a damaged cochlea or vestibule. Consequently, many studies have attempted to investigate the pathophysiology of Meniere's disease and the presence/absence of EH using histological examination in animal models, [11][12][13][14][15][16][17] with the most popular model being that induced by surgical ablation of the endolymphatic duct and sac. A surgical technique proposed by Kimura requires penetration of the brain's protective covering, leading to an increase in morbidity and mortality associated with penetration of the central nervous system.…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of the usefulness of three-dimensional optical coherence tomography in a guinea pig model of endolymphatic hydrops induced by surgical obliteration of the endolymphatic duct

et al. 2015

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Abstract. Optical coherence tomography (OCT) has advanced significantly over the past two decades and is currently used extensively to monitor the internal structures of organs, particularly in ophthalmology and dermatology. We used ethylenediamine tetra-acetic acid (EDTA) to decalcify the bony walls of the cochlea and investigated the inner structures by deep penetration of light into the cochlear tissue using OCT on a guinea pig model of endolymphatic hydrops (EH), induced by surgical obliteration of the endolymphatic duct. The structural and functional changes associated with EH were identified using OCT and auditory brainstem response tests, respectively. We also evaluated structural alterations in the cochlea using three-dimensional reconstruction of the OCT images, which clearly showed physical changes in the cochlear structures. Furthermore, we found significant anatomical variations in the EH model and conducted graphical analysis by strial atrophy for comparison. The physical changes included damage to and flattening of the organ of Corti-evidence of Reissner's membrane distention-and thinning of the lateral wall. These results indicate that observation of EDTA-decalcified cochlea using OCT is significant in examination of gradual changes in the cochlear structures that are otherwise not depicted by hematoxylin and eosin staining.

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Section: Discussionsupporting

confidence: 82%

Section: Introductionmentioning

confidence: 99%

Evaluation of the usefulness of three-dimensional optical coherence tomography in a guinea pig model of endolymphatic hydrops induced by surgical obliteration of the endolymphatic duct

et al. 2015

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“…In a previous study using an animal model of MD, Dunnebier et al 20 reported chronic ES dysfunction induced by destruction of the ES, and acute stress-induced endolymph production by aldosterone administration revealed severe degrees of hydrops in the cochlea. 20 In this investigation, we successfully developed a new clinically relevant animal model for MD, in which desmopressin (a VP type 2 receptor agonist) was administered after electrocauterization of the ES.…”

mentioning

confidence: 99%

“…20 In this investigation, we successfully developed a new clinically relevant animal model for MD, in which desmopressin (a VP type 2 receptor agonist) was administered after electrocauterization of the ES.…”

mentioning

confidence: 99%

Morphological and functional changes in a new animal model of Ménière's disease

Egami

Kakigi

Sakamoto

et al. 2013

Laboratory Investigation

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The purpose of this study was to clarify the underlying mechanism of vertiginous attacks in Ménière's disease (MD) while obtaining insight into water homeostasis in the inner ear using a new animal model. We conducted both histopathological and functional assessment of the vestibular system in the guinea-pig. In the first experiment, all animals were maintained 1 or 4 weeks after electrocauterization of the endolymphatic sac of the left ear and were given either saline or desmopressin (vasopressin type 2 receptor agonist). The temporal bones from both ears were harvested and the extent of endolymphatic hydrops was quantitatively assessed. In the second experiment, either 1 or 4 weeks after surgery, animals were assessed for balance disorders and nystagmus after the administration of saline or desmopressin. In the first experiment, the proportion of endolymphatic space in the cochlea and the saccule was significantly greater in ears that survived for 4 weeks after surgery and were given desmopressin compared with other groups. In the second experiment, all animals that underwent surgery and were given desmopressin showed spontaneous nystagmus and balance disorder, whereas all animals that had surgery but without desmopressin administration were asymptomatic. Our animal model induced severe endolymphatic hydrops in the cochlea and the saccule, and showed episodes of balance disorder along with spontaneous nystagmus. These findings suggest that administration of desmopressin can exacerbate endolymphatic hydrops because of acute V2 (vasopressin type 2 receptor)-mediated effects, and, when combined with endolymphathic sac dysfunction, can cause temporary vestibular abnormalities that are similar to the vertiginous attacks in patients with MD. Ménière's disease (MD) is a well-known inner ear disorder characterized by symptoms including recurring attacks of vertigo typically lasting for hours, fluctuating sensorineural hearing loss, and tinnitus. Since the milestone findings on the temporal bones of MD patients, 1,2 endolymphatic hydrops (EH) has been considered as the histopathological origin of MD, as characteristic morphological changes were reported to be produced by surgical obstruction of the endolymphatic sac (ES) in guinea-pig. 3 This observation indicates that malabsorption of endolymph in the ES is one of the possible mechanisms underlying the development of EH. This experimentally induced EH has been frequently used as an animal model to investigate the pathogenesis of inner ear disorders associated with EH. In terms of vestibular function, while canal dysfunction and/or spontaneous nystagmus have been occasionally observed in this animal model, 4,5 episodes of vertiginous attack or balance disorder have only rarely been observed. 6 In other words, this model is insufficient for fully representing the common symptoms of MD.Water homeostasis of the inner ear is essential for maintaining the functions of hearing and balance. EH is considered to be the result of disruption of inner ear water homeostasis, which ...

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“…Partant de cette hypothèse, des modèles expéri-mentaux dits «biphasiques» ont cherché à comparer les pourcentages d'hydrops endolymphatiques obtenus chez le cochon d'Inde soit par dysfonctionnement isolé du sac (diminution de la vascularisation et du drainage lymphatique), soit lorsque celui-ci est associé à d'autres perturbations de l'homéostasie de l'endolymphe. Une lésion isolée du sac entraîne un hydrops dans 40 % des cas, alors que ce pourcentage est de 90 % lorsqu'une administration de minéralocorticoïdes est associée [15], ces hormones étant supposées augmenter la sécrétion de l'endolymphe cochléo-vestibulaire. Hydrops endolymphatique au cours de la maladie de Ménière.…”

Section: Hydrops Endolymphatique Expérimentalunclassified