Two pathogens of Greenshell™ mussel larvae, Perna canaliculus: Vibrio splendidus and a V. coralliilyticus/neptunius‐like isolate

Kesarcodi-Watson, Aditya; Kaspar, Heinrich F.; Lategan, M. Josie; Gibson, L. F.

doi:10.1111/j.1365-2761.2009.01006.x

Cited by 87 publications

(64 citation statements)

References 23 publications

(33 reference statements)

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“…In addition, we use two-dimensional liquid chromatography coupled with tandem mass spectrometry and bioassays to investigate the influence of nonpathogenic (24 1C) and pathogenic (27 1C) temperatures on the expression of virulence Figure 1 Global distribution of V. coralliilyticus strains. The V. coralliilyticus strains represented here are (a) type strains (Ben-Haim et al, 2003a), as well as strains identified using (b) DnaJ PCR (Vezzulli et al, 2010), (c, d) 16S rRNA sequencing (Sussman et al, 2008;Kesarcodi-Watson et al, 2009), (e) multi-locus sequencing (Alves et al, 2010) and (f) multiple molecular analyzes, that is, 16S rRNA sequencing, recA PCR and repetitive extragenic palindromic -polymerase chain reaction (REP-PCR) (Vizcaino et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

“…coralliilyticus is of interest because of its global distribution, broad host range and temperaturedependent pathogenicity in corals. V. coralliilyticus has been isolated from marine organisms in the Atlantic (Ben-Haim et al, 2003a;Alves et al, 2010;Vizcaino et al, 2010), Indian (Ben-Haim et al, 2003a) and Pacific Oceans (Sussman et al, 2008;Kesarcodi-Watson et al, 2009), as well as the Mediterranean (Vezzulli et al, 2010) and Red Seas (Ben-Haim et al, 2003a) (Figure 1). It causes fatal infections in a wide range of organisms, including unicellular algae (Ben-Haim et al, 2003b;de Oliveira Santos et al, 2011), corals (Ben-Haim et al, 2003b), oysters (Jeffries, 1982), shrimp (Austin et al, 2005;de Oliveira Santos et al, 2011), rainbow trout (Austin et al, 2005) and flies (Alves et al, 2010;de Oliveira Santos et al, 2011) during experimental infection assays.…”

Section: Introductionmentioning

confidence: 99%

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Temperature regulation of virulence factors in the pathogen Vibrio coralliilyticus

Kimes

Grim

Johnson³

et al. 2011

The ISME Journal

226

200

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Sea surface temperatures (SST) are rising because of global climate change. As a result, pathogenic Vibrio species that infect humans and marine organisms during warmer summer months are of growing concern. Coral reefs, in particular, are already experiencing unprecedented degradation worldwide due in part to infectious disease outbreaks and bleaching episodes that are exacerbated by increasing SST. For example, Vibrio coralliilyticus, a globally distributed bacterium associated with multiple coral diseases, infects corals at temperatures above 27 1C. The mechanisms underlying this temperature-dependent pathogenicity, however, are unknown. In this study, we identify potential virulence mechanisms using whole genome sequencing of V. coralliilyticus ATCC (American Type Culture Collection) BAA-450. Furthermore, we demonstrate direct temperature regulation of numerous virulence factors using proteomic analysis and bioassays. Virulence factors involved in motility, host degradation, secretion, antimicrobial resistance and transcriptional regulation are upregulated at the higher virulent temperature of 27 1C, concurrent with phenotypic changes in motility, antibiotic resistance, hemolysis, cytotoxicity and bioluminescence. These results provide evidence that temperature regulates multiple virulence mechanisms in V. coralliilyticus, independent of abundance. The ecological and biological significance of this temperature-dependent virulence response is reinforced by climate change models that predict tropical SST to consistently exceed 27 1C during the spring, summer and fall seasons. We propose V. coralliilyticus as a model Gram-negative bacterium to study temperature-dependent pathogenicity in Vibrio-related diseases.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Temperature regulation of virulence factors in the pathogen Vibrio coralliilyticus

Kimes

Grim

Johnson³

et al. 2011

The ISME Journal

226

200

View full text Add to dashboard Cite

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“…Strains of V. coralliilyticus have been isolated from diseased corals in the Indian Ocean and Red Sea (4), the Caribbean (21), and the Great Barrier Reef and Micronesia (8), and strains have been shown to infect coral of the genera Pocillopora (20) and Pachyseris, Acropora, and Montipora (8). V. coralliilyticus has also been shown to infect noncoral hosts that include the mussel (Perna canaliculus) larva (22) and the rainbow trout (Oncorhynchus mykiss) (23).…”

mentioning

confidence: 99%

Vibrio coralliilyticus Strain OCN008 Is an Etiological Agent of Acute Montipora White Syndrome

Ushijima

Videau

Burger

et al. 2014

Appl Environ Microbiol

113

116

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dIdentification of a pathogen is a critical first step in the epidemiology and subsequent management of a disease. A limited number of pathogens have been identified for diseases contributing to the global decline of coral populations. Here we describe Vibrio coralliilyticus strain OCN008, which induces acute Montipora white syndrome (aMWS), a tissue loss disease responsible for substantial mortality of the coral Montipora capitata in Ka ne'ohe Bay, Hawai'i. OCN008 was grown in pure culture, recreated signs of disease in experimentally infected corals, and could be recovered after infection. In addition, strains similar to OCN008 were isolated from diseased coral from the field but not from healthy M. capitata. OCN008 repeatedly induced the loss of healthy M. capitata tissue from fragments under laboratory conditions with a minimum infectious dose of between 10 7 and 10 8 CFU/ml of water. In contrast, Porites compressa was not infected by OCN008, indicating the host specificity of the pathogen. A decrease in water temperature from 27 to 23°C affected the time to disease onset, but the risk of infection was not significantly reduced. Temperature-dependent bleaching, which has been observed with the V. coralliilyticus type strain BAA-450, was not observed during infection with OCN008. A comparison of the OCN008 genome to the genomes of pathogenic V. coralliilyticus strains BAA-450 and P1 revealed similar virulence-associated genes and quorum-sensing systems. Despite this genetic similarity, infections of M. capitata by OCN008 do not follow the paradigm for V. coralliilyticus infections established by the type strain.

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“…V. coralliilyticus has been previously isolated from marine organisms in the Atlantic 2,13 , Indian Ocean 2 , and Pacific Oceans 14,15 , Mediterranean Sea 16 and Red Sea 2 . Although it is uncertain whether this organism is a primary or opportunistic coral pathogen, evidence strongly suggests that this endemic member of the global coral holobionts 17 has a role in coral disease 18 .…”

Section: Samples From Two Different Estuaries (Cochin Andmentioning

confidence: 99%

Prevalence and Multiple Antibiotic Resistance of Vibrio coralliilyticus, along the Southwest Coast of India

Silvester¹,

Alexander²,

George³

et al. 2017

Current Science

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Two pathogens of Greenshell™ mussel larvae, Perna canaliculus: Vibrio splendidus and a V. coralliilyticus/neptunius‐like isolate

Cited by 87 publications

References 23 publications

Temperature regulation of virulence factors in the pathogen Vibrio coralliilyticus

Temperature regulation of virulence factors in the pathogen Vibrio coralliilyticus

Vibrio coralliilyticus Strain OCN008 Is an Etiological Agent of Acute Montipora White Syndrome

Prevalence and Multiple Antibiotic Resistance of <i>Vibrio coralliilyticus</i>, along the Southwest Coast of India

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