“…The Z granule contains two factors, ZNFX-1, and the Argonaute WAGO-4, which are essential for the inheritance of post-transcriptional silencing effects in the embryo, complementing the nuclear function Argonaute HRDE-1, which transmit heritable transcriptional silencing. [34,49] The mutator foci contain proteins involved in small RNA synthesis, including the RdRP and factors involved in RNA pUGylation, an RNA substrate that recruits RdRP to generate small RNAs essential for heritable RNAi. [44,45,50] Therefore, besides being a vehicle for maternal small RNA inheritance in the zygote, germ granules can also be necessary for reinitiating the de novo synthesis of small RNAs in embryos (Figure 1).…”