Two Oyster Species That Show Differential Susceptibility to Virus Infection Also Show Differential Proteomic Responses to Generic dsRNA

Abstract

Viral diseases are a significant cause of mortality and morbidity in oysters, resulting in significant economic losses. We investigated the proteomic responses of these two species of oysters to generic double-stranded RNAs (poly I:C and poly A:U). Analysis of proteomic data using isobaric tags for relative and absolute quantitaion (iTRAQ) indicated that there were significant differences in the proteomic responses of the two oyster species resulting from this treatment. Gene ontology analysis showed that several biological processes, cellular components, and molecular function were unique to the different data sets. For example, a number of proteins implicated in the TLR signaling pathway were associated with the Saccostrea glomerata data set but were absent in the Crassostra gigas data set. These results suggest that the differences in the proteomic responses to dsRNA may underpin the biological differences in viral susceptibility. Molecular targets previously shown to be expressed in C. gigas in response to OsHV1 infections were not present in our proteomic data sets, although they were present in the RNA extracted from the very same tissues. Taken together, our data indicate that there are substantial disparities between transcriptomic and proteomic responses to dsRNA challenge, and a comprehensive account of the oysters' biological responses to these treatments must take into account that disparity.

Temporal proteomic profiling reveals insight into critical developmental processes and temperature-influenced physiological response differences in a bivalve mollusc

“…While larger size could be due to elevated temperature increasing overall development rate [28,29] by increasing ingestion activity [11,30], we did not detect a significant time:temperature interaction effect. We did, however, observe specific time points where temperature appeared to have a greater impact on protein abundance ( dsRNA exposure response in adult Pacific oyster gill tissue [39]. Larval geoduck proteomic response to ciliates similarly showed an increase in immune response proteins including molecular chaperones and reactive oxygen species responders [40].…”

Temporal proteomic profiling reveals insight into critical developmental processes and temperature-influenced physiological response differences in a bivalve mollusc

“…For instance, Pacific oyster juveniles infected with OSHV-1 had significantly higher survival at 29 °C than 21 °C, potentially because at 29 °C they are able to alter their physiology to limit viral entry [ 47 , 48 ]. Specifically related to the proteomics results, viral recognition protein DEAD box protein 58 and growth inhibition/cell death-related proteins serine-threonine kinase receptor-associated protein and DnaJ-like protein have been implicated in dsRNA exposure response in adult Pacific oyster gill tissue [ 49 ]. Larval geoduck proteomic response to ciliates similarly showed an increase in immune response proteins including molecular chaperones and reactive oxygen species responders [ 50 ].…”

Long-lasting antiviral innate immune priming in the Lophotrochozoan Pacific oyster, Crassostrea gigas

“…Likewise, Masood et al . observed in the oyster Saccostrea glomerata , that poly(I:C) detection in the hemocytes decreased significantly from 15 h post-injection, also suggesting a short lifetime in oyster tissues 66 . These data suggest that immune priming may not be due to a persistence of poly (I:C) in oyster tissues.…”