Two Outbreaks of<i>Listeria monocytogenes</i>Infection, Northern Spain

Pérez-Trallero, Emilio; Zigorraga, Carmen; Artieda, Junkal; Alkorta, Miriam; Marimón, José María

doi:10.3201/eid2012.140993

Cited by 63 publications

(58 citation statements)

References 6 publications

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“…Based on somatic and flagellar antigens L. monocytogenes has 13 serotypes 1/2a, 1/2b, 1/2c, 3a, 3b, 3c, 4a, 4ab, 4b, 4c, 4d, 4e and three of them (1/2a, 1/2b, 4b) are more frequently related to human listeriosis (CLARK et al, 2010;FDA, 2012;CARTWRIGHT et al, 2013;PÉREZ-TRALLERO et al, 2014).…”

Section: Listeria Monocytogenesmentioning

confidence: 99%

An overview of Listeria monocytogenes contamination in ready to eat meat, dairy and fishery foods

2017

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Listeria monocytogenes is a relevant foodborne pathogen in public health, responsible for outbreaks of listeriosis often associated to the consumption of ready to eat meat, dairy and fishery products. Listeriosis is a serious disease that can lead to death and mainly affect children, the elderly and immunocompromised individuals. In pregnant women causes abortion or neonatal listeriosis. In Brazil, ready to eat food are appreciated and increasingly consumed by the population. Furthermore, products such as sausages, bologna, hams and cheeses have characteristics such as pH, Aw and sodium chloride content that favor the development of L. monocytogenes during their shelf life. The purpose of this paper was to present an overview of L. monocytogenes contamination in different meat, dairy and fishery products that are ready for consumption and thereby support the adoption of strategies to mitigate this risk, contributing to achieve the appropriate level protection for the consumers and thus strengthen Brazil's food safety system.

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Section: Listeria Monocytogenesmentioning

confidence: 99%

An overview of Listeria monocytogenes contamination in ready to eat meat, dairy and fishery foods

2017

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“…Contaminated foie gras from 3 unopened samples linked to a 2012-2013 Spain outbreak contained L. monocytogenes at 5.2 × 10 4 CFU/g (56). These data could help us gain better understanding of the risk associated with L.…”

Section: Discussionmentioning

confidence: 99%

Listeria monocytogenes in Stone Fruits Linked to a Multistate Outbreak: Enumeration of Cells and Whole-Genome Sequencing

Chen

Burall

Luo

et al. 2016

Appl Environ Microbiol

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In 2014, the identification of stone fruits contaminated with Listeria monocytogenes led to the subsequent identification of a multistate outbreak. Simultaneous detection and enumeration of L. monocytogenes were performed on 105 fruits, each weighing 127 to 145 g, collected from 7 contaminated lots. The results showed that 53.3% of the fruits yielded L. monocytogenes (lower limit of detection, 5 CFU/fruit), and the levels ranged from 5 to 2,850 CFU/fruit, with a geometric mean of 11.3 CFU/fruit (0.1 CFU/g of fruit). Two serotypes, IVb-v1 and 1/2b, were identified by a combination of PCR- and antiserum-based serotyping among isolates from fruits and their packing environment; certain fruits contained a mixture of both serotypes. Single nucleotide polymorphism (SNP)-based whole-genome sequencing (WGS) analysis clustered isolates from two case-patients with the serotype IVb-v1 isolates and distinguished outbreak-associated isolates from pulsed-field gel electrophoresis (PFGE)-matched, but epidemiologically unrelated, clinical isolates. The outbreak-associated isolates differed by up to 42 SNPs. All but one serotype 1/2b isolate formed another WGS cluster and differed by up to 17 SNPs. Fully closed genomes of isolates from the stone fruits were used as references to maximize the resolution and to increase our confidence in prophage analysis. Putative prophages were conserved among isolates of each WGS cluster. All serotype IVb-v1 isolates belonged to singleton sequence type 382 (ST382); all but one serotype 1/2b isolate belonged to clonal complex 5.IMPORTANCE WGS proved to be an excellent tool to assist in the epidemiologic investigation of listeriosis outbreaks. The comparison at the genome level contributed to our understanding of the genetic diversity and variations among isolates involved in an outbreak or isolates associated with food and environmental samples from one facility. Fully closed genomes increased our confidence in the identification and comparison of accessory genomes. The diversity among the outbreak-associated isolates and the inclusion of PFGE-matched, but epidemiologically unrelated, isolates demonstrate the high resolution of WGS. The prevalence and enumeration data could contribute to our further understanding of the risk associated with Listeria monocytogenes contamination, especially among high-risk populations.

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“…Over this period, outbreaks were additionally documented in Austria, Portugal, Spain, and Denmark (1, 5–8). Listeriosis has only recently been listed as a notifiable disease in Spain since March 2015 (9), and therefore, the annual incidence may be even higher (1–5, 10, 11). Reduced immunocompetency of listeriosis patients was recently reported as a major risk factor for fatal outcomes (12).…”

Section: Introductionmentioning

confidence: 99%

“…Two northern Spain regions, Gipuzkoa in the Basque country (~700,000 inhabitants) and Cantabria (~350,000 inhabitants), were selected to calculate annual incidence, since these communities have voluntarily reported a high number of listeriosis cases since 2008 (1, 4, 5, 10, 11). We compiled data on genetic epidemiology, clinical manifestations, virulence of clinical isolates, and immunological parameters in listeriosis patients over the period of a year (August 2014–September 2015).…”

Section: Introductionmentioning

confidence: 99%

Biomarker Tools to Design Clinical Vaccines Determined from a Study of Annual Listeriosis Incidence in Northern Spain

et al. 2016

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Two regions of northern Spain, Gipuzkoa, and Cantabria present high annual incidence of listeriosis (1.86 and 1.71 cases per 100,000 inhabitants, respectively). We report that the high annual incidences are a consequence of infection with highly virulent Listeria monocytogenes isolates linked to fatal outcomes in elderly patients with cancer. In addition, listeriosis patients with cancer present low IL-17A/IL-6 ratios and significantly reduced levels of anti-GAPDH1–22 antibodies, identified as two novel biomarkers of poor prognosis. Analysis of these biomarkers may aid in reducing the incidence of listeriosis. Moreover, GAPDH1–22-activated monocyte-derived dendritic cells of listeriosis patients with cancer seem useful tools to prepare clinical vaccines as they produce mainly Th1 cytokines.

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Two Outbreaks ofListeria monocytogenesInfection, Northern Spain

Cited by 63 publications

References 6 publications

An overview of Listeria monocytogenes contamination in ready to eat meat, dairy and fishery foods

An overview of Listeria monocytogenes contamination in ready to eat meat, dairy and fishery foods

Listeria monocytogenes in Stone Fruits Linked to a Multistate Outbreak: Enumeration of Cells and Whole-Genome Sequencing

Biomarker Tools to Design Clinical Vaccines Determined from a Study of Annual Listeriosis Incidence in Northern Spain

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