Two NPF transporters mediate iron long-distance transport and homeostasis in Arabidopsis

Chen, Si-Ying; Gu, Tianyu; Qi, Zi-Ai; Yan, Jun; Fang, Zijun; Lu, Yuting; Li, Hui; Gong, Ji-Ming

doi:10.1016/j.xplc.2021.100244

Cited by 14 publications

(12 citation statements)

References 53 publications

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“…As mentioned, NRT1.1 has various functions, including NO 3 À uptake, NO 3 À sensing, and auxin transport (Fang et al, 2021). Because of these multiple functions, NRT1.1 has pleiotropic effects on plant responses to various stresses, including N deficiency, Pi deficiency, K deficiency, acidic stress, NH 4 + toxicity, and Cd/Zn/Pb toxicity (Mao et al, 2014;Medici et al, 2015;Fang et al, 2016;Hu et al, 2019;Fang et al, 2020;Zhu et al, 2019;Maghiaoui et al, 2020;Pan et al, 2020;Ye, Tian, Zhou, Zhu, Du, et al, 2021;Ye et al, 2022;Wang, Wang, et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

“…Very recently, two NRTs, NPF5.9 and NPF5.8, were found to act as NA transporters to redundantly enhance root‐to‐shoot Fe transport under Fe starvation (Chao et al., 2021). Consequently, the npf5.8/npf5.9 double mutants were extremely sensitive to Fe deficiency (Chao et al., 2021; Chen et al., 2021). However, NRT1.1 is unable to transport Fe or NA (Chao et al., 2021), suggesting that the process of Fe transport is also not involved in the enhanced tolerance to Fe deficiency upon NRT1.1 disruption.…”

Section: Discussionmentioning

confidence: 99%

“…Alleviation of Fe deficiency chlorosis by disruption of NRT1.1 is not associated with auxin signaling and regulation of root Fe uptake NRT1.1 is a powerful transporter with pleiotropic functions, including NO 3 À uptake, NO 3 À sensing, and auxin transport (Fang et al, 2021;Wang, Hu, et al, 2020). These pleiotropic functions of NRT1.1 are largely controlled by phosphorylation of the Thr101 residue (Bouguyon et al, 2015).…”

Section: Iron Deficiency Downregulates Nrt11 Which Enhances Plant Tol...mentioning

confidence: 99%

“…Initially identified as a dual-affinity transporter involved in multiple phases of NO 3 À uptake (Liu et al, 1999;Liu & Tsay, 2003;Wang et al, 1998), a series of studies on NRT1.1 have demonstrated that the function of NRT1.1 is more than that of a simple NO 3 À transporter and that the other functions of NRT1.1 are independent of its NO 3 À uptake activity (Fang et al, 2021;Wang, Hu, et al, 2020). Through the analysis of the functional and phenotypic Ó 2022 Society for Experimental Biology and John Wiley & Sons Ltd.…”

Section: Introductionmentioning

confidence: 99%

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Inhibition of shoot‐expressed NRT1.1 improves reutilization of apoplastic iron under iron‐deficient conditions

Zhou

Zhu

et al. 2022

The Plant Journal

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SUMMARY Iron deficiency is a major constraint for plant growth in calcareous soils. The interplay between NO3− and Fe nutrition affects plant performance under Fe‐deficient conditions. However, how NO3− negatively regulates Fe nutrition at the molecular level in plants remains elusive. Here, we showed that the key nitrate transporter NRT1.1 in Arabidopsis plants, especially in the shoots, was markedly downregulated at post‐translational levels by Fe deficiency. However, loss of NRT1.1 function alleviated Fe deficiency chlorosis, suggesting that downregulation of NRT1.1 by Fe deficiency favors plant tolerance to Fe deficiency. Further analysis showed that although disruption of NRT1.1 did not alter Fe levels in both the shoots and roots, it improved the reutilization of apoplastic Fe in shoots but not in roots. In addition, disruption of NRT1.1 prevented Fe deficiency‐induced apoplastic alkalization in shoots by inhibiting apoplastic H+ depletion via NO3− uptake. In vitro analysis showed that reduced pH facilitates release of cell wall‐bound Fe. Thus, foliar spray with an acidic buffer promoted the reutilization of Fe in the leaf apoplast to enhance plant tolerance to Fe deficiency, while the opposite was true for the foliar spray with a neutral buffer. Thus, downregulation of the shoot‐part function of NRT1.1 prevents apoplastic alkalization to ensure the reutilization of apoplastic Fe under Fe‐deficient conditions. Our findings may provide a basis for elucidating the link between N and Fe nutrition in plants and insight to scrutinize the relevance of shoot‐expressed NRT1.1 to the plant response to stress.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Iron Deficiency Downregulates Nrt11 Which Enhances Plant Tol...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Inhibition of shoot‐expressed NRT1.1 improves reutilization of apoplastic iron under iron‐deficient conditions

Zhou

Zhu

et al. 2022

The Plant Journal

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show abstract

“… Wu et al. (2021b) revealed that hypoxia-triggered elevation of gamma-aminobutyric acid (GABA) restores membrane potential and maintains ion homeostasis in Arabidopsis , and Chen et al. (2021a) demonstrated that two Arabidopsis NPF transporters, NPF5.9 and NPF5.8, act redundantly to mediate long-distance Fe transport and homeostasis, independent of their nitrate transport function.…”

mentioning

confidence: 99%