Two Novel Mycoviruses Related to Geminivirus Isolated from the Soil-Borne Fungi<i>Macrophomina Phaseolina</i>(Tassi) Goid. and<i>Mucor Racemosus</i>Bull

Hafez, Elsayed E.; Aseel, Dalia G.; Mostafa, Saad

doi:10.5504/bbeq.2013.0086

Cited by 3 publications

(4 citation statements)

References 30 publications

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“…Mycoviruses are currently classified into 23 families and one unclassified genus ( Figure 1 ) by the ICTV according to their genome type and organization; (accessed on 27 March 2023). The majority of mycoviruses studied to date have dsRNA or ssRNA genomes, however, a few mycoviruses with ssDNA genomes have also been found [ 12 , 52 , 53 , 175 , 328 ].…”

Section: Diversity and Taxonomymentioning

confidence: 99%

“…ssDNA mycoviruses are not exclusively found in Genomoviridae , however. For example, geminivirus-like ssDNA has also been discovered in Macrophomina phaseolina (M. phaseolina DNA virus; MpDV) and Mucor racemosus (M. racemosus DNA virus; MrDV) [ 53 ]. Additionally, a novel ssDNA mycovirus, Gigaspora circovirus A has also been discovered and belongs to the Circoviridae family but has not yet been assigned to the genus [ 52 ].…”

Section: Diversity and Taxonomymentioning

confidence: 99%

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Fungal Viruses Unveiled: A Comprehensive Review of Mycoviruses

Hough

Steenkamp

Wingfield

et al. 2023

Viruses

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Mycoviruses (viruses of fungi) are ubiquitous throughout the fungal kingdom and are currently classified into 23 viral families and the genus botybirnavirus by the International Committee on the Taxonomy of Viruses (ICTV). The primary focus of mycoviral research has been on mycoviruses that infect plant pathogenic fungi, due to the ability of some to reduce the virulence of their host and thus act as potential biocontrol against these fungi. However, mycoviruses lack extracellular transmission mechanisms and rely on intercellular transmission through the hyphal anastomosis, which impedes successful transmission between different fungal strains. This review provides a comprehensive overview of mycoviruses, including their origins, host range, taxonomic classification into families, effects on their fungal counterparts, and the techniques employed in their discovery. The application of mycoviruses as biocontrol agents of plant pathogenic fungi is also discussed.

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Section: Diversity and Taxonomymentioning

confidence: 99%

Section: Diversity and Taxonomymentioning

confidence: 99%

Fungal Viruses Unveiled: A Comprehensive Review of Mycoviruses

Hough

Steenkamp

Wingfield

et al. 2023

Viruses

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“…In a recent study, Myers et al (2020) found 27.1% of the 107 Mucoromycota fungi screened by cellulose chromatography or transcriptome-mining positive for mycoviruses [8]. Previously, virus-like particles (VLPs) were detected in five Rhizopus isolates [9], a geminivirus-like ssDNA virus was described in Mucor racemosus [10], and a (−) ssRNA virus was detected in Mucor irregularis [11]. Recently, four viruses with dsRNA genomes were detected and characterized in Umbelopsis ramanniana [7], and two (+) ssRNA narnaviruses were reported from Rhizopus microsporus [12].…”

Section: Introductionmentioning

confidence: 99%

Characterization of Four Novel dsRNA Viruses Isolated from Mucor hiemalis Strains

Kartali

Nyilasi

Kocsubé́

et al. 2021

Viruses

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We previously screened the total nucleic acid extracts of 123 Mucor strains for the presence of dsRNA molecules without further molecular analyses. Here, we characterized five novel dsRNA genomes isolated from four different Mucor hiemalis strains with next-generation sequencing (NGS), namely Mucor hiemalis virus 1a (MhV1a) from WRL CN(M) 122; Mucor hiemalis virus 1b (MhV1b) from NRRL 3624; Mucor hiemalis virus 2 (MhV2) from NRRL 3616; and Mucor hiemalis virus 3 (MhV3) and Mucor hiemalis virus (MhV4) from NRRL 3617 strains. Genomes contain two open reading frames (ORF), which encode the coat protein (CP) and the RNA dependent RNA polymerase (RdRp), respectively. In MhV1a and MhV1b, it is predicted to be translated as a fusion protein via -1 ribosomal frameshift, while in MhV4 via a rare +1 (or−2) ribosomal frameshift. In MhV2 and MhV3, the presence of specific UAAUG pentanucleotide motif points to the fact for coupled translation termination and reinitialization. MhV1a, MhV2, and MhV3 are part of the clade representing the genus Victorivirus, while MhV4 is seated in Totivirus genus clade. The detected VLPs in Mucor strains were from 33 to 36 nm in diameter. Hybridization analysis revealed that the dsRNA molecules of MhV1a-MhV4 hybridized to the corresponding molecules.

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“…named as Mucorales RNA virus 1 (MucRV1). In addition, a geminivirus-like ssDNA virus was described and characterized in Mucor racemosus (Hafez et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Detection and Molecular Characterization of Novel dsRNA Viruses Related to the Totiviridae Family in Umbelopsis ramanniana

Kartali

Nyilasi

Szabo

et al. 2019

Front. Cell. Infect. Microbiol.

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Umbelopsis ramanniana is an oleaginous fungus belonging to the Mucoromycotina subphylum. Our group had previously detected four double-stranded RNA (dsRNA) bands in the U. ramanniana NRRL 1296 strain by gel electrophoresis. Here we describe the molecular characterization of its dsRNA elements as well as the discovery of four novel dsRNA viruses: Umbelopsis ramanniana virus 1 (UrV1), Umbelopsis ramanniana virus 2 (UrV2), Umbelopsis ramanniana virus 3 (UrV3), and Umbelopsis ramanniana virus 4 (UrV4). Full genomes of UrV1, UrV3, and UrV4 were determined using the full-length amplification of cDNAs (FLAC) technique; they contain two open reading frames (ORF), which putatively encode the coat protein (CP) and the RNA dependent RNA polymerase (RdRp), respectively. In case of UrV2, a partial ORF encoding a partial RdRp gene could be determined. Based on the phylogeny inferred from the RdRp sequences, UrV1 and UrV4 belong to the genus Totivirus , while UrV2 may belong to the genus Victorivirus . UrV3 nested to a novel, unclassified group of Totiviridae , which is related to the genus Totivirus . Hybridization analysis revealed that the dsRNA molecules of UrV1 and UrV4 correspond to the same 5.0-kbp electrophoretic band, whilst the probe for the UrV3 hybridized to the largest, 5.3-kbp and the 3.0-kbp bands of the dsRNA pattern of U. ramanniana . Interestingly, the probe for the UrV2 sequence did not hybridized to any dsRNA bands, but it could be amplified from the isolated 3.0-kbp fragment. By transmission electron microscopy, two different isometric virus particles with about 50 and 35 nm in diameter were detected in U. ramanniana NRRL 1296 indicating that this strain harbor multiple viruses. Beside U. ramanniana , dsRNA elements were also detected in other Umbelopsis isolates with different patterns consisting of 2 to 4 discrete and different sized (0.7–5.3-kbp) dsRNA molecules. Based on a hybridization analysis with UrV1 CP and RdRp probes, the bands with the size of around 5.0-kbp, which were present in all tested Umbelopsis strains, are presumed as possible full mycovirus genomes.

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Two Novel Mycoviruses Related to Geminivirus Isolated from the Soil-Borne FungiMacrophomina Phaseolina(Tassi) Goid. andMucor RacemosusBull

Abstract: ABSTRACT

Cited by 3 publications

References 30 publications

Fungal Viruses Unveiled: A Comprehensive Review of Mycoviruses

Fungal Viruses Unveiled: A Comprehensive Review of Mycoviruses

Characterization of Four Novel dsRNA Viruses Isolated from Mucor hiemalis Strains

Detection and Molecular Characterization of Novel dsRNA Viruses Related to the Totiviridae Family in Umbelopsis ramanniana

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