Two novel cell culture models of buccal mucosal oral cancer from patients with no risk-habits of tobacco smoking or chewing

Vipparthi, Kavya; Patel, Ankit Kumar; Ghosh, Subhashis; Das, Subrata; Das, Chitrarpita; Das, Koyeli; Sarkar, Anwesha; Thatikonda, Venu; Pal, Biswajoy; Remani, K. N.; Arora, Neeraj; Parihar, Mayur; Vijayakumar, Maleppillil Vavachan; Bhat, Manoj Kumar; Boppana, Ramanamurthy; Bhattacharjee, Samsiddhi; Biswas, Nidhan K.; Arun, Pattatheyil; Sharan, Rajeev; Singh, Sandeep

doi:10.1016/j.oraloncology.2020.105131

Cited by 11 publications

(13 citation statements)

References 54 publications

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“…These include leukoplakia, erythroplakia, and changes in the palate due to smoking (5). Because a significant association has been observed between smoking and oral cancers, in areas where smoking is high, the need to assess precancerous lesions is greater and more important (21). Therefore, by examining and diagnosing these lesions in a timely manner, cancer can be prevented in these patients.…”

Section: Discussionmentioning

confidence: 99%

“…Since the study of the oral precancerous lesions is one of the research priorities, the present study was performed to identify and prevent these lesions promptly through clinical studies and evaluating P53 gene expression, to solve the economic and psychological problems of patients who suffer from more advanced lesions due that 65 patients (58.5%) had leukoplakia (22). It is noteworthy that the patients were only accustomed to smoking cigarettes and did not use any other smoking (21).…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of P53 gene expression by immunohistochemistry to diagnosis oral precancerous lesions

Wang

Hao

2021

Cell Mol Biol (Noisy-le-grand)

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Oral Precancerous lesions include leukoplakia, erythroplakia, and mucosa palate changes due to reverse smoking. Assessing the prevalence of these lesions in a cross-sectional study can be effective in the timely prevention and treatment of lesions, in any community. Hence, in the present study, evaluation of P53 gene expression was done by immunohistochemistry method to diagnosis oral precancerous lesions. For this purpose, 111 Chinese patients (54 women and 57 men) were selected for examination. The age range of these patients was 22 to 69 years, and their average age was 32.6 years. All patients were examined by one physician. Oral mucosa was used for immunohistochemical evaluations. All samples taken from patients' mucosa were evaluated by one pathologist under a light microscope. 80 cases of the 111 patients were smokers and 27 were non-smokers. Among the 80 smokers, 56.25% had leukoplakia, 3.75% had erythroplakia, and 40% had mucosa palate changes. Regarding non-smokers, 74.07% had leukoplakia and 25.93% had erythroplakia. None of the non-smokers had mucosa palate changes. In terms of the lesion location, in patients with leukoplakia 89.23%, and patients with erythroplakia 90% of the lesion was located in the cheek mucosa and buccal vestibule. Also, in patients with leukoplakia 9.23%, and patients with erythroplakia 10% of the lesion was located in the lips vestibular mucosa. Only 1.54% of leukoplakia had a lesion in the vermilion border, and none of the erythroplakia patients had a lesion on the vermilion border. 76 patients (68.46%) showed positive expression of the P53 gene. The expression level of the P53 gene did not show a significant relationship with age, and the genders did not have a statistically significant difference in terms of gene expression. The expression level of the P53 gene was 59.8% in leukoplakia, 70% in erythroplakia, and 40% in Mucosa palate changes. The present study showed that the evaluation of P53 gene expression was well able to detect oral precancerous lesions and their severity by increasing their expression rate.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Evaluation of P53 gene expression by immunohistochemistry to diagnosis oral precancerous lesions

Wang

Hao

2021

Cell Mol Biol (Noisy-le-grand)

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“…Oral cancer cell lines, GBC02 and GBC035 used in this study were recently established by us and maintained as described [44]. Other oral cancer cell lines SCC-029, SCC-070, SCC-084 and SCC-032 were kindly provided by Dr. Susanne M. Gollin, University of Pittusburgh, USA [45].…”

Section: Methodsmentioning

confidence: 99%

“…This study (EC/GOVT/01/12) was approved by the Institutional ethics committee of National Institute of Biomedical Genomics (NIBMG) and the institutional review board of Tata Medical Center (TMC), Kolkata, India. Primary tumor tissue samples were collected and processed as previously described by us [44,46]. Following enzymatic digestion of tumor tissues using 1x…”

Section: Dissociation Of Primary Oral Tumor Cellsmentioning

confidence: 99%

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Spontaneous differentiation leads to emergence of hybrid cell states relate to poor prognosis in oral cancer

Vipparthi

Hari

Chakraborty

et al. 2021

Preprint

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Cancer cells within individual tumors often exist in distinct phenotypic states contributing to intratumoral heterogeneity (ITH). However, studies on cell state dynamics among oral cancer cells are largely missing. Here, we have multiplexed phenotypic markers of putative oral-stem-like cancer cells (SLCCs) and characterized diversity among CD44-positive oral cancer cell subpopulations with respect to distinct expression of CD24 and aldehyde dehydrogenase (ALDH)-activity. Our in vitro experimental observations were explained by a Markov model where subpopulations followed two distinct patterns of spontaneous repopulation dynamics. Cells showed stochastic inter-conversions on ALDH-axis, harnessed by cancer cells to enrich ALDHHigh subpopulations in response to Cisplatin treatment. However, these cells followed a strict non-interconvertible transition of CD24Low to CD24High subpopulations, even in response to chemotherapy-induced stress. Using phenotype-specific RNAseq, we suggest the organization of subpopulations into hierarchical structure with possible maintenance of intermediate alternate states of stemness within the differentiating oral cancer cells. We also show that the population dynamics described here may influence tumor behaviour by increasing ITH in aggressive oral tumors. Overall, the described phenotypic subgroups not only reliably exhibited spontaneous or Cisplatin-driven cellular dynamics but also the distinct transcription states of oral cancer cells. Most importantly, our in vitro model system derived observations emphasized the prognostic power which may be translated for betterment of oral cancer patients.Graphical AbstractWe have characterized diversity among CD44-positive oral cancer cells lines with respect to distinct expression of CD24 and ALDH-activity. Cells showed stochastic inter-conversions on ALDH-axis but a strict non-interconvertible transition of CD24Low to CD24High phenotype, even in response to chemotherapy-induced stress. RNAseq study suggested the organization of subpopulations into hierarchical structure with possible maintenance of intermediate alternate states of stemness within the differentiating oral cancer cells. The described population dynamics may influence tumor behaviour by increasing intratumoral heterogeneity in aggressive oral tumors.

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Recent and advanced therapy for oral cancer

Baskar

Palaniyandi

Viswanathan

et al. 2023

Biotech & Bioengineering

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Oral cancer is a common and deadly kind of tissue invasion, has a high death rate, and may induce metastasis that mostly affects adults over the age of 40. Most in vitro traditional methods for studying cancer have included the use of monolayer cell cultures and several animal models. There is a worldwide effort underway to reduce the excessive use of laboratory animals since, although being physiologically adequate, animal models rarely succeed in exactly mimicking human models. 3D culture models have gained great attention in the area of biomedicine because of their capacity to replicate parent tissue. There are many benefits to using a drug delivery approach based on nanoparticles in cancer treatment. Because of this, in vitro test methodologies are crucial for evaluating the efficacy of prospective novel nanoparticle drug delivery systems. This review discusses current advances in the utility of 3D cell culture models including multicellular spheroids, patient‐derived explant cultures, organoids, xenografts, 3D bioprinting, and organoid‐on‐a‐chip models. Aspects of nanoparticle‐based drug discovery that have utilized 2D and 3D cultures for a better understanding of genes implicated in oral cancers are also included in this review.

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Two novel cell culture models of buccal mucosal oral cancer from patients with no risk-habits of tobacco smoking or chewing

Cited by 11 publications

References 54 publications

Evaluation of P53 gene expression by immunohistochemistry to diagnosis oral precancerous lesions

Evaluation of P53 gene expression by immunohistochemistry to diagnosis oral precancerous lesions

Spontaneous differentiation leads to emergence of hybrid cell states relate to poor prognosis in oral cancer

Recent and advanced therapy for oral cancer

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