Two new triterpenoid saponins fromPulsatilla cernua(Thunb.) Bercht. et Opiz

Abstract

Two new triterpenoid saponins, named cernuaside C and D, have been isolated from Pulsatilla cernua (Thunb.) Bercht. et Opiz. The structures of the two new triterpenoid saponins were elucidated as 3-O-beta-D-xylopyranosyl(1 --> 2)-[alpha-L-rhamnopyranosyl(1 --> 3)]-alpha-L-arabinopyranosyl oleanolic acid 28-alpha-L-rhamnopyranosyl(1 --> 4)-beta-D-glucopyranosyl(1 --> 6)-beta-D-glucopyranoside. (1) and 3-O-alpha-L-arabinopyranosyl (1 --> 3)-alpha-L-rhamnopyranosyl(1 --> 2)-alpha-L-arabinopyranosyl oleanolic acid 28-O-beta-D-glucopyranoside (2) by 1D, 2D-NMR techniques, ESI-MS analysis as well as chemical methods.

Cytotoxic Oleanane-Type Triterpenoid Saponins from the Rhizomes of Anemone rivularis var. flore-minore

“…The analysis of the NMR data (Table 1 and Table 2) indicated that 3 was a saponin containing one triterpene aglycone and six monosaccharides. The aglycone of 3 was recognized to be oleanolic acid by 1 H- and 13 C-NMR analysis (Table 1), which was in full agreement with literature data [21]. The chemical shifts of C-3 ( δ C = 88.6) and C-28 ( δ C = 176.5) revealed that 3 was a bisdesmosidic glycoside.…”

“…The arabinose unit was determined to have an α-anomeric configuration on the basis of the 3 J H-1/H-2 (7.1 Hz) value observed in the 4 C 1 form. Although the anomeric proton of the rhamnose moiety was observed as a singlet in the 1 H-NMR spectrum, the 13 C-NMR shift of Rha C-5 at δ C = 69.5 indicated the α - anomeric configuration [20,21]. All proton signals due to sugars were identified by careful analysis of the 1 H- 1 H COSY, TOCSY and NOESY spectra, and the carbon signals were assigned by HSQC and further confirmed by HMBC spectra (Table 1).…”

Exploring glycolate oxidase (GOX) as an antiurolithic drug target: Molecular modeling and in vitro inhibitor study

“…Epidemiological studies are also convincing and shown positive correlations. The major constituents of Tt are steroidal saponins [11][12][13][14][15][16][17][18][19][20][21][22][23][24][25][26][27][28] and flavonoids [29,30]. Although the crude extracts of Tt was found to be effective in kidney stone management, a detailed study of its contents for therapeutic intervention of kidney stone is not sufficiently explored yet.…”

Triterpenoid Saponins from Anemone rivularis var. Flore-Minore and Their Anti-Proliferative Activity on HSC-T6 Cells

“…The Glc unit was determined to have a β anomeric configuration on the basis of its 3 J H-1/H-2 coupling constant (8.2 Hz). Although the anomeric proton of the Rha moiety was observed as a singlet in the 1 H-NMR spectrum, the 13 C-NMR shift of Rha C-5 at δ C = 69.6 indicated the α anomeric configuration [21,22]. The complete assignments of proton signals belonging to sugars were based on 2D NMR of 1 H- 1 H COSY, TOCSY, and NOESY, and the carbon signals were assigned by HSQC and further confirmed by the HMBC spectrum (Table 2).…”