Two new techniques for quantitative determination of Leishmania amastigotes

Cenini, Pietro; Reeve, Anne M.; Neal, R. A.

doi:10.1016/0035-9203(89)90639-1

Cited by 10 publications

(3 citation statements)

References 4 publications

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“…(1989). The proportion of viable amastigotes was estimated with fluorescent illumination after staining with ethidium bromide and fluorescein diacetate (Cenini ET AL, 1989), and the sus pension was diluted with inactivated rabbit blood to give an estimated 3 x 10 5 viable amastigotes per 1.0 ml. The sandflies were permitted to feed on the suspen sion through a hamster cheek-pouch attached to a glass feeder warmed by circulating water (Bastien, 1990).…”

Section: Infection Of Sandfliesmentioning

confidence: 99%

Metacyclic promastigotes ofLeishmaniain the salivary glands of experimentally infected phlebotomine sandflies

Killick‐Kendrick

Tang

et al. 1996

Parasite

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Summary:Thirty one female Phlebotomus duboscqi experimentally infected with Leishmania tropica were found with metacyclic promastigotes in their salivary glands. The presence of parasites in the glands was correlated with heavy infections of metacyclic promastigotes in the stomodaeal valve and thoracic midgut of the fly and it is suggested that the parasites may have migrated through the gut wall into the glands which lie against the valve in the thorax of the fly. No evidence was found that gland invasion is an obligatory stage of the life-cycle of Leishmania, but reports of wild-caught flies with parasites in the glands, coupled with these laboratory observations, raise the possibility that regurgitation of parasites or the migration of metacyclic promastigotes into the mouthparts may not be the only mechanisms of transmission by bite.

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Section: Infection Of Sandfliesmentioning

confidence: 99%

Metacyclic promastigotes ofLeishmaniain the salivary glands of experimentally infected phlebotomine sandflies

Killick‐Kendrick

Tang

et al. 1996

Parasite

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“…In order to clarify these questions, we analysed mRNA expression of IFN-γ and IL-4, both in spleen and liver of co-infected C57BL/6 mice, by semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR). Cenini et al (1989).…”

mentioning

confidence: 99%

Studies in a co-infection murine model of Plasmodium chabaudi chabaudi and Leishmania infantum: interferon<FONT FACE=Symbol>-g</FONT> and interleukin-4 mRNA expression

Marques

Rolão

Centeno-Lima

et al. 2005

Mem. Inst. Oswaldo Cruz

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“…Mice were killed by cervical dislocation and tissue samples were obtained from skin, liver, spleen, and testes for gross pathology and for histopathological examination using routine haematoxylin and eosin (H&E) staining techniques. Quantitative determination of amastigotes in the skin was done 30 and 45 days after infection using a modification of fluorescein diacetate/ethidium bromide viability test [8].…”

Section: Methodsmentioning

confidence: 99%

Histopathological Study of Three Leishmania Major Strains

Amin

Khedr

1999

med

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Amastigotes count in inoculated foot pad and histopathologicaJ study of infected mice were performed to study three Leishmania major strains: the Senegalese strain MHOM/SN/OOIDKI(SN), the Sudanese strain MHOMlSO/89/UG8(SO), and the Saudi strain MHOMlSAl84/JISH(SA).Eight-week-old male BALB/c mice were divided into three groups. Each group was infected with one of the studied strains. Amastigotes in the inoculated foot of infected. mice in the three groups were counted at 30 and 45 days using modified fluorescence diacetate/ethidium bromide viability test, The number of amastigotes in the SA group was found to be significantly lower than that of the other two groups at both durations. There was no significant difference between the number of amastigotes in the SN and SO strains at 30 days, but it was significantly higher in the SN group than that of the SD group at 45 days post-inoculation. In the animals studied, tissue samples were obtained from the skin, liver, spleen, and testes for gross pathological changes and histopathological evaluation. The SO strain started earlier and led a more aggressive course. The SN and SA strains were less aggressive. The SA strain showed changes at an earlier time and resulted in a more aggressive course of the disease. The SN and SA strains were less aggressive. The SA strain showed the most chronic course of the three.

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Two new techniques for quantitative determination of Leishmania amastigotes

Cited by 10 publications

References 4 publications

Metacyclic promastigotes ofLeishmaniain the salivary glands of experimentally infected phlebotomine sandflies

Metacyclic promastigotes ofLeishmaniain the salivary glands of experimentally infected phlebotomine sandflies

Studies in a co-infection murine model of Plasmodium chabaudi chabaudi and Leishmania infantum: interferon<FONT FACE=Symbol>-g</FONT> and interleukin-4 mRNA expression

Histopathological Study of Three Leishmania Major Strains

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