Two New Species of Fusarium: Fusarium brevicatenulatum from the Noxious Weed Striga asiatica in Madagascar and Fusarium pseudoanthophilum from Zea mays in Zimbabwe

Nirenberg, Helgard I.; O’Donnell, Kerry; Kroschel, J.; Andrianaivo, Alain Paul; Frank, J.M.; Mubatanhema, Wellington

doi:10.2307/3761404

Cited by 28 publications

(11 citation statements)

References 6 publications

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“…The availability of molecular diagnostics for mating type also may enable the analysis of purportedly asexual fungi, e.g., F. oxysporum, and 12 of the 13 recently described Fusarium taxa (15,16). There is circumstantial evidence in F. oxysporum for sexual reproduction in the form of high levels of diversity with respect to the multilocus vegetative compatibility trait (see, for example, references 8, 11, 12, and 22), especially in populations of putatively nonpathogenic strains.…”

Section: Discussionmentioning

confidence: 99%

“…Fusarium isolates in the Gibberella fujikuroi species complex include important fungal pathogens of agricultural crops and trees and may be divided into at least eight different biological species and 32 additional asexual species or phylogenetic lineages (4,10,13,15,16). All sexually fertile species in the G. fujikuroi species complex are dimictic, i.e., two isolates are cross-fertile if they carry the different mating-type idiomorphs MAT-1 and MAT-2 (4, 6, 9, 13), which share no sequence similarity with respect to either DNA sequence or the proteins encoded (5).…”

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PCR-Based Identification of MAT-1 and MAT-2 in the Gibberella fujikuroi Species Complex

Steenkamp

Wingfield

Coutinho

et al. 2000

Appl Environ Microbiol

154

106

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All sexually fertile strains in the Gibberella fujikuroi species complex are heterothallic, with individual mating types conferred by the broadly conserved ascomycete idiomorphs MAT-1 and MAT-2. We sequenced both alleles from all eight mating populations, developed a multiplex PCR technique to distinguish these idiomorphs, and tested it with representative strains from all eight biological species and 22 additional species or phylogenetic lineages from this species complex. In most cases, either an ϳ800-bp fragment from MAT-2 or an ϳ200-bp fragment from MAT-1 is amplified. The amplified fragments cosegregate with mating type, as defined by sexual cross-fertility, in a cross of Fusarium moniliforme (Fusarium verticillioides). Neither of the primer pairs amplify fragments from Fusarium species such as Fusarium graminearum, Fusarium pseudograminearum, and Fusarium culmorum, which have, or are expected to have, Gibberella sexual stages but are thought to be relatively distant from the species in the G. fujikuroi species complex. Our results suggest that MAT allele sequences are useful indicators of phylogenetic relatedness in these and other Fusarium species.Fusarium isolates in the Gibberella fujikuroi species complex include important fungal pathogens of agricultural crops and trees and may be divided into at least eight different biological species and 32 additional asexual species or phylogenetic lineages (4, 10, 13, 15, 16). All sexually fertile species in the G. fujikuroi species complex are dimictic, i.e., two isolates are cross-fertile if they carry the different mating-type idiomorphs MAT-1 and MAT-2 (4, 6, 9, 13), which share no sequence similarity with respect to either DNA sequence or the proteins encoded (5). The MAT-2 idiomorphs thus far characterized have a conserved HMG (high-mobility-group) domain (3,5,6,9). The MAT-1 idiomorphs have a conserved ␣-domain (5, 25), but no MAT-1 alleles have yet been described from any members of the G. fujikuroi species complex.Our objectives in this study were (i) to sequence conserved portions of the MAT-1 and MAT-2 alleles from the eight known G. fujikuroi mating populations, (ii) to develop a multiplex PCR reaction to be used for the identification of both mating-type idiomorphs within the defined biological species of the G. fujikuroi species complex, (iii) to determine the range of Fusarium species which have Gibberella teleomorphs to which this technique can be successfully applied, and (iv) to test the use of the sequence of the MAT idiomorphs for phylogenetic analyses. This technique eases the identification of strains to be used in crosses to identify new biological species and eliminates the need for sexual crosses to score this trait. MAT sequence variations may be used as phylogenetic characters, providing another marker that can be used to test phylogenies for robustness.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

PCR-Based Identification of MAT-1 and MAT-2 in the Gibberella fujikuroi Species Complex

Steenkamp

Wingfield

Coutinho

et al. 2000

Appl Environ Microbiol

154

106

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“…Separate Gibberella species names have been assigned to all but one of these mating populations (47,55), and numerous additional Fusarium anamorphs within the Liseola and Elegans sections have been delineated on the basis of morphology and/or DNA sequence differences, e.g., those reported by Marasas et al (28)(29)(30), Nirenberg and O'Donnell (40), and Nirenberg et al (41). Collectively, these Fusarium species have a worldwide distribution and cause disease on a wide variety of agricultural and horticultural plants (for reviews, see references 21 and 22).…”

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Species Diversity of and Toxin Production by Gibberella fujikuroi Species Complex Strains Isolated from Native Prairie Grasses in Kansas

Leslie

Zeller

Logrieco

et al. 2004

Appl Environ Microbiol

104

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Fusarium species from agricultural crops have been well studied with respect to toxin production and genetic diversity, while similar studies of communities from nonagricultural plants are much more limited. We examined 72 Fusarium isolates from a native North American tallgrass prairie and found that Gibberella intermedia (Fusarium proliferatum), Gibberella moniliformis (Fusarium verticillioides), and Gibberella konza (Fusarium konzum) dominated. Gibberella thapsina (Fusarium thapsinum) and Gibberella subglutinans (Fusarium subglutinans) also were recovered, as were seven isolates that could not be assigned to any previously described species on the basis of either morphological or molecular characters. In general, isolates from the prairie grasses produced the same toxins in quantities similar to those produced by isolates of the same species recovered from agricultural hosts. The G. konza isolates produce little or no fumonisins (up to 120 g/g by one strain), and variable but generally low to moderate amounts of beauvericin (4 to 320 g/g) and fusaproliferin (50 to 540 g/g). Toxicity to Artemia salina larvae within most species was correlated with the concentration of either beauvericin or fusaproliferin produced. Organic isolates from some cultures of G. moniliformis were highly toxic towards A. salina even though they produced little, if any, beauvericin or fusaproliferin. Thus, additional potentially toxigenic compounds may be synthesized by G. moniliformis strains isolated from prairie grasses. The Fusarium community from these grasses appears to contain some species not found in surrounding agricultural communities, including some that probably are undescribed, and could be capable of serving as a reservoir for strains of potential agricultural importance.

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“…Recently, these MPs have been reassigned to Gibberella species names parallel to the names of their anamorphs, and additional anamorphs have also been identified (5,(26)(27)(28)47). These Fusarium species have been isolated from different hosts such as maize, rice, sugarcane, pine, mango, and sorghum (20) and cause several serious plant diseases.…”

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Functional Characterization of Two Cytochrome P450 Monooxygenase Genes, P450 - 1 and P450 - 4 , of the Gibberellic Acid Gene Cluster in Fusarium proliferatum ( Gibberella fujikuroi MP-D)

Malonek

Rojas

Hedden

et al. 2005

Appl Environ Microbiol

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Two New Species of Fusarium: Fusarium brevicatenulatum from the Noxious Weed Striga asiatica in Madagascar and Fusarium pseudoanthophilum from Zea mays in Zimbabwe

Cited by 28 publications

References 6 publications

PCR-Based Identification of MAT-1 and MAT-2 in the Gibberella fujikuroi Species Complex

PCR-Based Identification of MAT-1 and MAT-2 in the Gibberella fujikuroi Species Complex

Species Diversity of and Toxin Production by Gibberella fujikuroi Species Complex Strains Isolated from Native Prairie Grasses in Kansas

Functional Characterization of Two Cytochrome P450 Monooxygenase Genes, P450 - 1 and P450 - 4 , of the Gibberellic Acid Gene Cluster in Fusarium proliferatum ( Gibberella fujikuroi MP-D)

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