Two new sheep haemoglobins, one of which is replaced by haemoglobin C in anaemia

Kilgour, L.; Dixon, S. C.; Tucker, Elizabeth M.

doi:10.1111/j.1365-2052.1990.tb03215.x

Cited by 15 publications

(12 citation statements)

References 12 publications

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“…The HBX allele was added in the hemoglobin-b system tested. It is presumed that the hemoglobin-b component coded by this allele is electrophoretically similar to that of the HBH allele, which was reported by Kilgour et al (1990).…”

Section: Discussionmentioning

confidence: 83%

“…Polyacrylamide gradient gel electrophoresis was used for the transferrin system (Ghane et al, 1977), horizontal starch-gel electrophoresis for the arylesterase and X-protein systems (Tsunoda et al, 1984;Tucker et al, 1967), polyacrylamide-gel isoelectric focusing for the hemoglobin-b system (Kilgour et al, 1990), and an atomic absorption flame emission spectrophotometer (Shimazu, AA-6200) or compact ion densitometer (Horiba, Cardy Ion) for the potassium transport system. The staining procedures for detecting transferrin, hemoglobin-b, and X-protein components were conducted according to routine methods using Coomassie brilliant blue R-250 or G. Arylesterase activity was detected using a-naphthyl acetate as a substrate and fast blue RR salt as a dye.…”

Section: Protein and Nonprotein Phenotypingmentioning

confidence: 99%

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Phylogenetic Relationships among Indigenous Sheep Populations in East Asia based on Five Informative Blood Protein and Nonprotein Polymorphisms

Tsunoda

Chang²,

Sun³

et al. 2006

Biochem Genet

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The phylogenetic relationships among 23 local sheep breeds and varieties in East Asia were determined based on allele frequency data for five informative and polymorphic blood protein and nonprotein loci (transferrin, arylesterase, hemoglobin-beta, X-protein, and potassium transport) using electrophoresis and ion-densitometric techniques. Comparatively striking differences in the frequency distributions, especially of hemoglobin-beta and X-protein alleles, were seen between the northern population of the Khalkhas, Bhyanglung, Baruwal, Jakar, Sakten, and five Chinese breeds and the southern population of the Bengal, Kagi, Lampuchhre, Myanmar, and Sipsu breeds. Clustering analyses using UPGMA and NJ methods and principal component analysis, using the data of all populations and loci tested, clearly demonstrated the difference. The two population groups are divided by the boundary of the Himalayas, and each diverged into three subgroups: Mongolian, Tibetan, and Himalayan groups in the north and Indian groups I, II, and III in the south. It was noted that the genetic differentiation of populations was more distinct in the northern group. These findings strongly suggest the existence of at least two large and phylogenetically different gene pools of sheep in East Asia.

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Section: Discussionmentioning

confidence: 83%

Section: Protein and Nonprotein Phenotypingmentioning

confidence: 99%

Phylogenetic Relationships among Indigenous Sheep Populations in East Asia based on Five Informative Blood Protein and Nonprotein Polymorphisms

Tsunoda

Chang²,

Sun³

et al. 2006

Biochem Genet

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“…Analysis of lysates of the Sardinian breeds by IEF indicated that one animal was of the Hb AA and 16 were of the Hb AB phenotype, the Figure 1 also indicates that the px chain has a different mobility from that of the PH, the latter being not separated with respect to the normal PB (Kilgour et al 1990). Application of lysates containing both the Hb B and Hb X to the urea polyacrylamide gel electrophoresis at alkaline pH, which allowed the PE chain to be separated from the pB (John & John 1977), resulted in a single @-chain band.…”

Section: G E N O M E Analysismentioning

confidence: 98%

“…In the present study, of all the enzymes used, DraI, EcoRI and BamHI proved to be even more informative in giving rise to GSHP. Hb C is produced under anaemia conditions only by sheep of the Hb A type (van Vliet & Huisman 1964) as is the case of the Hb G (Kilgour et al 1990). As expected, the pattern was found to be distinctly different in all the species compared with cow and buffalo genomes.…”

Section: G E N O M E Analysismentioning

confidence: 99%

Localization of the porcine growth hormone gene to chromosome 12pl.2 p1–5

Yerle

Lahbib-Mansais

et al. 1993

Animal Genetics

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The gene encoding the porcine growth hormone (GH) has been localized to the q-arm of chromosome 1 2 using high-resolution R-banded chromosomes for in situ hybridization. We report here the localization of GH on the p-arm of this chromosome when using in situ hybridization on high-resolution G-banded chromosomes. Sequential Q-and R-banding show that this discrepancy is caused by a reversed orientation of chromosome 12 in the R-banded high-resolution karyotype published by Rsnne et al. (1987) and the G-banded standard karyotype.

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“…Polyacrylamide gradient gel electrophoresis was used for the transferrin system (Ghane et al 1977), horizontal starch-gel electrophoresis for the arylesterase and X-protein systems (Tsunoda et al 1984;Tucker et al 1967), polyacrylamide gel isoelectric focusing for the hemoglobin-b system (Kilgour et al 1990), and a compact ion densitometer (Horiba, Cardy Ion) for the potassium transport system. The staining procedures for detecting transferrin, hemoglobin-b, and X-protein components were conducted according to routine methods using Coomassie brilliant blue R-250 or G. Arylesterase activity was detected using a-naphthyl acetate as a substrate and fast blue RR salt as a dye.…”

Section: Protein and Nonprotein Phenotypingmentioning

confidence: 99%

Phylogeny of Local Sheep Breeds in East Asia, Focusing on the Bayanbulak Sheep in China and the Sipsu Sheep in Bhutan

Tsunoda

Chang²,

Chang³

et al. 2009

Biochem Genet

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The phylogenetic positions of the Bayanbulak sheep in China and the Sipsu sheep in Bhutan in the northern Asian sheep group were determined on the basis of allele frequency data for five informative and polymorphic loci of blood protein and nonproteins, such as transferrin (TF), arylesterase (ES), hemoglobin-beta (HB-beta), X-protein (XP), and potassium transport (KE), using different electrophoretic and ion-densitometric techniques. Based on Nei's genetic distance, clustering analysis by the UPGMA method showed that the Bayanbulak sheep is clustered in the northern Asian sheep group. Furthermore, the Bayanbulak sheep belongs to a subgroup containing the Khalkhas and Hu sheep of the Mongolian sheep group, which is distinguished from another subgroup of the small-tailed Han, Tan, Tong, and Wadi sheep. The Bayanbulak sheep was closest to the Hu sheep, despite a morphological difference in the fat deposits. In addition to these findings, the Sipsu sheep was verified to belong to the Baruwal sheep.

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Two new sheep haemoglobins, one of which is replaced by haemoglobin C in anaemia

Cited by 15 publications

References 12 publications

Phylogenetic Relationships among Indigenous Sheep Populations in East Asia based on Five Informative Blood Protein and Nonprotein Polymorphisms

Phylogenetic Relationships among Indigenous Sheep Populations in East Asia based on Five Informative Blood Protein and Nonprotein Polymorphisms

Localization of the porcine growth hormone gene to chromosome 12pl.2 p1–5

Phylogeny of Local Sheep Breeds in East Asia, Focusing on the Bayanbulak Sheep in China and the Sipsu Sheep in Bhutan

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