Two new sesquiterpenoids from the rhizomes of<i>Nardostachys jatamansi</i>

Rekha, K.; Rao, R. Ranga; Pandey, Rashmi; Prasad, K. Rajendra; Babu, K. Suresh; Vangala, Janakiram R.; Kalivendi, Sashi V; Rao, J. Madhusudana

doi:10.1080/10286020.2012.738673

Cited by 16 publications

(6 citation statements)

References 11 publications

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“…The present study aimed to investigate the antioxidant and anticancer activity of the whole extract and subsequent fractions of N. jatamansi in breast cancer cells. Few research groups have evaluated the cytotoxic activity of N. jatamansi extract in various cancer cell lines [ 23 - 25 ], however, the mechanism of action of antiproliferative activity has not been investigated in breast cancer till date. These prompted us to investigate the anticancer potential of NJM extract and identify the most bioactive fraction with antiproliferative potential against ER-positive and ER-negative breast carcinoma cells and decipher the possible mechanisms of antiproliferative activity.…”

Section: Resultsmentioning

confidence: 99%

“…Sesquiterpenes are a promising class of natural compounds in cancer drug discovery with 3 compounds; artemisinin, thapsigargin and parthenolide currently being evaluated in clinical trials [ 58 ]. Other anticancer terpenoids from N. jatamansi include ursolic acid and 3-O-arabinosyl ursolic acid isolated from the cholorform:methanol fraction that showed cytotoxicity in lung (A-549), prostate (DU-145), breast cancer (MCF-7), and neuroblastoma (SK-N-SH) cells (IC 50 : 18–32 mM) [ 25 ]. Hence, the anticancer activity of the whole extract NJM and NJDE fraction against breast cancer cells could be attributed to the presence of a variety of compounds.…”

Section: Resultsmentioning

confidence: 99%

“…Bhagat et al ., have reported the cytotoxicity of alcoholic extract and n-butanol fraction of N. jatamansi in lung, liver, ovary and prostate cancer cell lines [ 24 ]. Moreover, two new sesquiterpenoids have been isolated from the roots and rhizomes of N. jatamansi and cytotoxicity of the crude chloroform:methanol extract and the isolates have been studied in lung, prostate, ER-positive breast cancer and neuroblastoma cell lines [ 23 , 25 ].…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of antioxidant and anticancer activity of extract and fractions of Nardostachys jatamansi DC in breast carcinoma

Chaudhary

Chandrashekar

Pai

et al. 2015

BMC Complement Altern Med

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BackgroundNardostachys jatamansi DC is a Himalayan medicinal herb that has been described in various traditional systems of medicine for its use in cancer. In view of its traditional claims, and chemical constituents, antioxidant and anticancer activities were evaluated in breast carcinoma.MethodsPetroleum ether (NJPE), methanol extract (NJM) and subsequent diethyl ether (NJDE), ethyl acetate (NJEA) and aqueous (NJAQ) fractions of roots and rhizomes of N. jatamansi were prepared. Total phenolic, flavonoid content, and antioxidant activities were determined using suitable methods. Antiproliferative activity was assessed in estrogen receptor (ER)-positive (MCF-7) and ER-negative breast carcinoma (MDA-MB-231) cells by MTT and SRB assay. Cell cycle analysis, Hoechst staining, and clonogenic assay were employed to determine the mode of antiproliferative and pro-apoptotic activity in MDA-MB-231 cells.ResultsNJM/fractions exhibited prominent antioxidant activity with significant correlation between phenolic content and ABTS (IC50) scavenging (R = −0.9680, P < 0.05), and total antioxidant capacity (R = 0.8396, P > 0.05). In MTT assay, NJM exhibited the highest antiproliferative activity (IC50: 58.01 ± 6.13 and 23.83 ± 0.69 μg/mL in MCF-7 and MDA-MB-231 respectively). Among the fractions, NJPE and NJDE were found to be most potent in MCF-7 (IC50: 60.59 ± 4.78 μg/mL) and MDA-MB-231 (IC50: 25.04 ± 0.90 μg/mL) cells respectively. Statistical analyses revealed NJM and NJDE exhibited significantly higher (P < 0.05) cytotoxicity in MDA-MB-231 cells. Cell cycle analysis demonstrated that NJM, NJPE and NJEA caused G2/M arrest while NJDE caused G0/G1 phase arrest in MDA-MB-231 cells. Further, NJM/fractions induced significant (P < 0.001) cell death by apoptosis characterized by apoptotic morphological changes in Hoechst staining and inhibited long-term proliferation (P < 0.001) of MDA-MB-231 cells in clonogenic assay. Lupeol and β-sitosterol were identified as anticancer principles in NJM/fractions by HPTLC.ConclusionOur results suggest that NJM/fractions possess significant antiproliferative potential which is mediated through cell cycle perturbation and pro-apoptotic effects in MDA-MB-231 cells. Moreover, this study highlights the antioxidant potential of NJM/fractions which can be attributed to the presence of phenols. NJDE emerged as the most potent fraction and further mechanistic and phytochemical investigations are under way to identify the active principles.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Evaluation of antioxidant and anticancer activity of extract and fractions of Nardostachys jatamansi DC in breast carcinoma

Chaudhary

Chandrashekar

Pai

et al. 2015

BMC Complement Altern Med

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“…The tetrazolium-based MTT assay was carried out as described in [29][30][31] with minor modifications. Briefly, 2 × 10 4 cells/well were seeded into 96-well plates.…”

Section: Mtt Assays For Measuring Cell Viabilitymentioning

confidence: 99%

BET Inhibitors Synergize with Carfilzomib to Induce Cell Death in Cancer Cells via Impairing Nrf1 Transcriptional Activity and Exacerbating the Unfolded Protein Response

2020

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Currently, proteasome inhibitors bortezomib, carfilzomib, and ixazomib are successfully used in clinics to treat multiple myeloma. However, these agents show limited efficacy against solid tumors. Identification of drugs that can potentiate the action of proteasome inhibitors could help expand the use of this therapeutic modality to solid tumors. Here, we found that bromodomain extra-terminal (BET) family protein inhibitors such as JQ1, I-BET762, and I-BET151 synergize with carfilzomib in multiple solid tumor cell lines. Mechanistically, BET inhibitors attenuated the ability of the transcription factor Nrf1 to induce proteasome genes in response to proteasome inhibition, thus, impeding the bounce-back response of proteasome activity, a critical pathway by which cells cope with proteotoxic stress. Moreover, we found that treatment with BET inhibitors or depletion of Nrf1 exacerbated the unfolded protein response (UPR), signaling that was initiated by proteasome inhibition. Taken together, our work provides a mechanistic explanation behind the synergy between proteasome and BET inhibitors in cancer cell lines and could prompt future preclinical and clinical studies aimed at further investigating this combination.

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“…N. jatamansi is belonging to Valerianaceae family; it is a small, hairy, perennial, erect, and rhizomatous herb. Phytochemical screening has revealed that it contained a large amount of bioactive compounds such as alkaloids, sesquiterpenes, lignans, coumarins, and steroids that are used for medicinal purposes [21,22]. This plant is appreciated for its antioxidant, antibacterial, stimulant, sedative, antifungal, antidiabetic, neuroprotective, hepatoprotective, anticancer, and cardioprotective properties [23][24][25].…”

Section: Introductionmentioning

confidence: 99%

Antioxidant and Anticancer Potentials of Biofabricated Silver Nanoparticles

Das

Rebecca

2017

Asian J Pharm Clin Res

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Objective: The objective of this study is to explore a rapid, bio-inspired approach to synthesize silver nanoparticles (AgNPs) using aqueous Nardostachys jatamansi leaf extract and evaluate its antioxidant and cytotoxic activities on human colon carcinoma (HCT-116) cell lines. Methods:The biosynthesized nanoparticles were analyzed using ultraviolet-visible spectrophotometer, scanning electron microscope (SEM), energy dispersive X-ray, X-ray diffractometer (XRD), and Fourier transform infrared spectroscopy. Free radical scavenging and cytotoxic studies were carried out at different concentrations of AgNPs (20-100 μg/mL) using antioxidant 2,2-diphenyl-1-picrylhydrazil (DPPH) and mitochondrial function assay methods.Results: Surface plasmon resonance spectrum at 434 nm confirmed the formation of AgNPs. SEM images show biosynthesized AgNPs are mostly spherical shaped within the range of 30.0-58.7 nm. XRD analysis reveals the crystallographic face-centered cubic structure of the AgNPs. Thus, synthesized metal nanoparticles were tested for antioxidant activity by DPPH assay, and anticancer activity was validated by lactate dehydrogenase leakage assay. Significant antioxidant property was observed as compared to standard L-ascorbic acid. Further, AgNPs showed a linear dose-response relationship against HCT-116 cell lines with increasing concentration of AgNPs. At a concentration of 20 μg/mL, AgNPs were able to inhibit the cell line's growth by less than 9.8 ± 0.7%, whereas 100 μg/mL of AgNPs significantly inhibited the cell line's growth greater than 90.4 ± 0.25%. Conclusion:The synthesized AgNPs were found to be highly stable and had significant antioxidant and anticancer activity against HCT-116 cell lines. It has wide applications in the biomedical field and can be produced with eco-friendly, rapid scale-up, and easy downstream processing.

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Two new sesquiterpenoids from the rhizomes ofNardostachys jatamansi

Cited by 16 publications

References 11 publications

Evaluation of antioxidant and anticancer activity of extract and fractions of Nardostachys jatamansi DC in breast carcinoma

Evaluation of antioxidant and anticancer activity of extract and fractions of Nardostachys jatamansi DC in breast carcinoma

BET Inhibitors Synergize with Carfilzomib to Induce Cell Death in Cancer Cells via Impairing Nrf1 Transcriptional Activity and Exacerbating the Unfolded Protein Response

Antioxidant and Anticancer Potentials of Biofabricated Silver Nanoparticles

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