Two new records of ascomycetes from Japan, Pyrenopeziza protrusa and P. nervicola (Helotiales, Dermateaceae sensu lato)

Itagaki, Hiroaki; Nakamura, Yasuhide; Hosoya, Tsuyoshi

doi:10.1016/j.myc.2019.02.008

Cited by 9 publications

(17 citation statements)

References 6 publications

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“…Otani (1989) enumerated the Japanese mycobiota of Discomycetes, a taxon no longer used but still practical in the modern taxonomy, and enumerated 77 genera with 214 species in the order. Through our exploration and enumeration, we added 57 newly documented taxa in Japan, and 30 new taxa, including 2 genera (Otani, Hosoya, & Furuya, 1991;Hosoya et al, 1993;Kubono & Hosoya, 1994;Hosoya & Otani 1995, 1997a, 1997b, 1997cHosoya & Harada 1999;Hosoya, 2000Hosoya, , 2002Hosoya, , 2004Hosoya, , 2005Hosoya, , 2009Hosoya & Huhtinen 2002;Narumi-Saito, Hosoya, Sano, & Harada, 2006;Hosoya et al, 2010aHosoya et al, , 2010cHosoya et al, , 2011Hosoya, Sung, Han, & Shin, 2010d;Hosoya et al, 2012;Hosoya, Hosaka, Saito, Degawa, & Suzuki, 2013a;Hosoya et al, 2013b;Hosoya, Saito, & Sasagawa, 2013c;Zhao, Hosoya, Shirouzu, Kakishima, & Yamaoka, 2013;Han et al, 2014a;Hosoya, Zhao, & Degawa, 2014;Degawa et al, 2015;Gross, Hosoya, Zhao, & Baral, 2015;Itagaki, Nakamura, & Hosoya, 2019;Tochihara & Hosoya, 2019). These included new species, new genera of both sexual and asexual states, now being merged under single names (some examples are presented in Fig.…”

Section: Advances In Helotialean Mycobiota Exploitation In Japanmentioning

confidence: 99%

“…Geoglossaceae has been almost completely unstudied since Imai (1934) and is now treated as an independent class apart from Helotiales ( Schoch et al, 2009 ). Orbiliaceae has been almost unstudied with a few exceptions ( Nagao, 1996 ), and it is now known as a separate group ( Orbiliomycetes ) from Helotiales and is recognized as a primitive member of the apothecial fungi ( Eriksson, Baral, Currah, Hansen, & Kurtzman, 2003 ; James et al, 2006 ). Studies are largely limited to Dermateaceae , Hemiphacidiaceae , and Leotiaceae .…”

Section: Systematicsmentioning

confidence: 99%

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Systematics, ecology, and application of Helotiales: Recent progress and future perspectives for research with special emphasis on activities within Japan

Hosoya

2021

Mycoscience

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Helotiales is one of the most diverse groups of apothecial ascomycetes, including 3000-4000 taxa. Recent progress in the systematics, ecology, and their applications through research is herein reviewed based on the experiences of the author with a special emphasis on activities in Japan. In the past 30 y, more than 50 helotialean taxa have been added to the mycobiota of Japan, including new taxa. With the advent of molecular phylogeny, some families have been revisited, such as members with stroma (Sclerotiniaceae and Rutstroemiaceae) or hairs (Hyaloscyphaceae and Lachnaceae). Although the monophyly of Helotiales has not yet been demonstrated, our understanding of its phylogeny has greatly advanced. The unexpected ecological nature represented by endophytism has been revealed through barcoding and other molecular techniques. The research history of ash dieback is also reviewed, and the endophytism/saprophytism of the pathogen on its original host is discussed. Drug discoveries within Helotiales are reviewed, and successful examples are presented. As future perspectives, both the cumulation of occurrence and sequence data of Helotiales is greatly encouraged to elucidate this important group of fungi.

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Section: Advances In Helotialean Mycobiota Exploitation In Japanmentioning

confidence: 99%

Section: Systematicsmentioning

confidence: 99%

Systematics, ecology, and application of Helotiales: Recent progress and future perspectives for research with special emphasis on activities within Japan

Hosoya

2021

Mycoscience

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“…DNA was extracted from the obtained isolates, amplified using polymerase chain reaction (PCR), and sequenced using the ITS1F/ ITS4 primer pair following the protocol described by Itagaki et al (2019). The obtained sequences were assembled using ATGC v.7.0.3 software (Genetyx, Tokyo, Japan) and basic local alignment search tool (BLAST) searches in the National Center for Biotechnology Information (NCBI) database (https://blast.ncbi.nlm.nih.…”

Section: Isolation and Molecular Identificationmentioning

confidence: 99%

Lifecycle of Pyrenopeziza protrusa (Helotiales, Dermateaceae sensu lato) in Magnolia obovata revealed by field observation and molecular quantification

Itagaki

Hosoya

2021

Mycoscience

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Fungi exhibit saprophytic, parasitic, and symbiotic lifestyles, and flexibly switching between them by the environmental changes and host conditions. However, only a few studies have elucidated the detailed changes in fungal DNA or morphology, including the formation of reproductive structures along with lifestyle switching. We hypothesized that Pyrenopeziza protrusa, which occurs abundantly and specifically on Magnolia obovata as a saprophyte, is also associated with living hosts and switches its lifestyles as part of its lifecycle. To elucidate this hypothesis, we periodically sampled the fresh/fallen leaves of M. obovata to observe the seasonal occurrence of reproductive structures for the isolation and detection/quantification of P. protrusa DNA with newly developed species-specific primers. The isolation frequency and amount of P. protrusa DNA drastically increased in the fresh leaves just before defoliation in autumn, but remained high in fallen leaves from autumn to spring. Abundant production of conidiomata and apothecia was also observed in the fallen leaves with increasing DNA content. These results clarified a large part of the lifecycle of P. protrusa, suggesting that the lifestyle is switched from symbiotic to saprophytic stage by significantly increasing the amount of DNA in response to host conditions according to the seasonal variations.

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“…The isolates were cultured in 2 ml of 2% malt extract (Difco). DNA extraction and polymerase chain reaction (PCR) using primers ITS1F and ITS4 15 for the ITS-5.8S sequence region were performed as described by Itagaki et al 16 .…”

Section: Culture and Sequencingmentioning

confidence: 99%

First report of emerging snake fungal disease caused by Ophidiomyces ophiodiicola from Asia in imported captive snakes in Japan

Takami¹,

Une

Mitsui

et al. 2020

Preprint

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Snake fungal disease (SFD) (Ophidiomycosis) is an emerging infectious disease caused by the fungus Ophidiomyces ophiodiicola which has been affecting wild and captive snakes in North America, Europe, and Australia. We report the cases of 12 imported captive colubrid snakes in Japan suspected of having SFD. Pathological and microbiological examinations were performed, and the results confirmed the diagnosis of SFD in two snakes, which indicated that the remaining sympatrically raised snakes also had SFD since they exhibited similar lesions. The oral administration of ciprofloxacin in addition to itraconazole had a significant treatment effect. This is the first report of SFD in Asia caused by O. ophiodiicola. To prevent the expansion of SFD in the natural environment in Japan, there is a need to evaluate the SFD carrier status of imported snakes, the pathogenicity of the infection in native snakes, and the prevalence and distribution of SFD in wild and captive snakes. Measures also must be taken to prevent endemicity globally.

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Two new records of ascomycetes from Japan, Pyrenopeziza protrusa and P. nervicola (Helotiales, Dermateaceae sensu lato)

Cited by 9 publications

References 6 publications

Systematics, ecology, and application of <i>Helotiales</i>: Recent progress and future perspectives for research with special emphasis on activities within Japan

Systematics, ecology, and application of <i>Helotiales</i>: Recent progress and future perspectives for research with special emphasis on activities within Japan

Lifecycle of <i>Pyrenopeziza protrusa</i> (<i>Helotiales</i>, <i>Dermateaceae</i> sensu lato) in <i>Magnolia obovata</i> revealed by field observation and molecular quantification

First report of emerging snake fungal disease caused by Ophidiomyces ophiodiicola from Asia in imported captive snakes in Japan

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