Two new rare species of Candolleomyces with pale spores from China

Abstract

Most species of Candolleomyces have brown or dark brown spores. Although pale-spored members are rare in the genus we frequently collected two such species from many Provinces during our investigations in subtropical China from 2016–2020. As revealed by morphological characterisation and multigene phylogenetic analyses (ITS LSU β-tub and tef-1α) these species which we have named C. subcacao and C. subminutisporus are unique and distinct from known taxa. In addition a new combination C. cladii-marisci is proposed on the basis of ITS sequence analysis of the type specimen. Detailed descriptions colour photos illustrations and a key to related species are presented.

Candolleomyces asiaticus sp. nov. (Psathyrellaceae, Agaricales), a novel species from Punjab, Pakistan

“…Candolleomyces asiaticus sp. nov. is also phylogenetically related to C. subcacao but differs from it by its bigger basidiospores (6.3-9.1 × 4-6.3 μm) (vs 6.8-8.8 × 3.9-4.9 μm in C. subcacao; Bau & Yan 2021). A comparison of diagnostic characters of phylogenetically close species is also given in Table 2.…”

Three new species of Candolleomyces (Agaricomycetes, Agaricales, Psathyrellaceae) from the Yanshan Mountains in China

“…According to the research of Wächter and Melzer (2020) , the species of Candolleomyces may be more abundant than previously thought and better delimitation of species boundaries is required. While the boundaries of some species are disputed, the number of new taxa is steadily increasing ( Sicoli et al 2019 ; Büttner et al 2020 ; Bau and Yan 2021 ). However, the continued discovery of clear boundaries in new taxa like this study enhances our comprehension of species in this genus.…”

“…The measurements and Q values are given as (a)b–c(d), in which “a” is the lowest value, “b–c” covers a minimum of 90% of the values and “d” is the highest value. Q stands for the ratio of length and width of a spore ( Bau and Yan 2021 ). Nomenclatural details were submitted to the MycoBank.…”

“…PCRs were performed in a volume of 25 μl consisted of 2 μl of DNA template; 1 μl of (10 μM) per primer; 12.5 μl 2 × Master Mix [Mei5 Biotechnology, Co., Ltd, China]. PCR amplification conditions refer to Bau and Yan (2021) . DNA sequences were sequenced by Zhongkexilin Biotechnology, Co., Ltd, Beijing, China.…”

“…Currently, there are 25 recognised species in Candolleomyces in the Index Fungorum website ( http://www.indexfungorum.org , until Jan. 2022) and 10 species were reported in China ( Yan 2018 ; Bau and Yan 2021 ).…”