Two new loci for hybrid sterility in cultivated rice (Oryza sativa L.)

Wan, Jian Min; Yamaguchi, Y.; Kato, Hitoshi; Ikehashi, Hiroshi

doi:10.1007/s001220050113

Cited by 18 publications

(21 citation statements)

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“…The 13 QTL which showed significant effects in CSSL and hybrids also represented an interesting genetic resource in the context of inbred or hybrid improvement via a design-breeding approach, as described by Wang et al (2007). The QTL on chromosomes 5 (qGWP5.1, qGPP5) and 7 (qGWP7.2, qGPP7.2) negatively affected GWP and GPP, perhaps because of tightly linkage with hybrid sterility genes such as S-7, S-24(t) or S-31 (Wan et al 1996;Kubo et al 1999b;Zhao et al 2007). The use of small chromosome segments in these regions might facilitate the identification of favorable linked QTL by avoiding the sterility in indica 9 japonica hybrids.…”

Section: Heterosis Of Yield-related Traitsmentioning

confidence: 99%

Identification of Indica rice chromosome segments for the improvement of Japonica inbreds and hybrids

Wang

Liu

et al. 2012

Theor Appl Genet

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Exploitation of heterosis has brought significant advance in plant breeding and agricultural production, although its genetic basis is still poorly understood. In this study, a total of 66 chromosome segment substitution (CSS) lines, derived from a cross between japonica rice inbred line Asominori (as the recurrent parent) and indica rice inbred line IR24 (as the donor parent), were used to investigate the genetic basis of heterosis in indica × japonica inter-subspecific rice hybrids. Each CSS line was crossed with the background parent Asominori, and the heterosis of F(1) hybrids was estimated by comparing the F(1) performance with its two parental lines. Field experiments were carried out across six different environments to evaluate yield and yield-related traits in the 66 CSS lines and their 66 corresponding F(1) hybrids. Quantitative trait loci (QTL) analyses were conducted using a likelihood ratio test based on the stepwise regression. Thirty-six QTL were identified with significant effects in CSSL, 21 with significant effects in hybrids and 13 with significant effects in both. On the basis of average dominance degree, of all the 70 QTL affecting yield-related agronomic traits, 28.6% (20) showed an overdominance, 35.7% (25) a partial dominance and 30% (21) an additive effect, indicating that all effects contribute to trait variation in japonica-indica rice hybrids. Effects of these QTL were examined to identify Indica rice chromosome segments of interest for the improvement of japonica inbred lines and hybrids.

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Section: Heterosis Of Yield-related Traitsmentioning

confidence: 99%

Identification of Indica rice chromosome segments for the improvement of Japonica inbreds and hybrids

Wang

Liu

et al. 2012

Theor Appl Genet

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“…Ikehashi et al [5,6] considered the female gamete as the main sterility form and proposed single locus S-5 controlling the hybrid fertility of the subspecies. Subsequently a series of female and male sterility loci were identified in different researches [7][8][9][10][11][12][13][14][15][16][17] . Zhang and Lu [18] found three F 1 pollen sterility loci by diallel analysis of Taichung 65 and its near-isogenic lines.…”

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confidence: 99%

Fine mapping of locus S-b for F1 pollen sterility in rice (Oryza sativa L.)

Zeng

Zhang

et al. 2006

CHINESE SCI BULL

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Hybrid sterility is the main barrier in utilizing the heterosis of subspecies in rice. A knowledge of the underlying molecular mechanism of the hybrid sterility will be useful for overcoming the barrier. In this research, the F 1 pollen sterility locus, S-b, was mapped between SSR markers PSM8 and PSM202. To fine map the locus, one F 2 mapping population of 3910 plants was developed using the near-isogenic lines of the locus. Ninety-seven recombinants between two markers were selected. Moreover, a series of markers, including two SSR markers, two InDel markers and four CAPS markers, were developed on the region. Linkage analysis showed that marker W4 was co-segregated with locus S-b, while makers A8 and A14 were located on the two sides of the locus with a distance of 0.026 and 0.038 cM, respectively. The markers were then integrated with the sequences of the clones of the region. Results showed that all the polymorphic markers were anchored on the three end-to-end jointed clones AC093089, AC079021 and AC134931. According to the physical information of the markers, locus S-b was finally delimited to a region of 27 kb between A8 and A14. Seven ORFs were identified on the region based on the annotation results of RiceGAAS system. These results laid the foundation for further cloning the gene.Firstly, the recombinants were selected from the large population by assaying the genotypes of the tightly linked markers. Then the selected recombinants were subjected to pollen fertility examination which was confirmed again in the F Keywords: hybrid sterility, molecular marker, fine mapping, rice.Strong heterosis existed in the hybrid of the subspecies in rice [1,2] . However, the partial sterility of the hybrid hinders the utilization of the heterosis [3,4] . Ikehashi et al. [5,6] considered the female gamete as the main sterility form and proposed single locus S-5 controlling the hybrid fertility of the subspecies. Subsequently a series of female and male sterility loci were identified in different researches [7][8][9][10][11][12][13][14][15][16][17] . Zhang and Lu [18] found three F 1 pollen sterility loci by diallel analysis of Taichung 65 and its near-isogenic lines. Thereafter other three F 1 pollen sterility loci were identified by testcross between the near-isogenic lines of Taichung 65 and a large number of varieties [19] . Of the six F 1 pollen sterility loci identified so far, three loci, namely S-a [20] , S-b [21] and S-c [22,23] , have been mapped and one locus, S-c, has been fine mapped [24] .Locus S-b was primary mapped on the short arm of chromosome 5 and tightly linked with markers RM13 and R830STS by screening the published microsatellite markers and developing cleaved amplified polymorphic sequence (CAPS) markers [25] . Rice genome sequencing project [26][27][28][29] provided abundant sequences for developing sequence-based microsatellite markers [30,31] and Insertion-Deletion (InDel) markers [32] . To map-based clone locus S-b, a large mapping population was developed and several putative ORFs were identi...

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“…The spikelet fertility of parents, F 1 , backcross population were determined for each plant by counting fertile and sterile spikelets on the upper half of three panicles after maturity, as described by Wan et al (1996).…”

Section: Plant Cell Repmentioning

confidence: 99%

Fine mapping of S37, a locus responsible for pollen and embryo sac sterility in hybrids between Oryza sativa L. and O. glaberrima Steud

Shen

Zhao

et al. 2015

Plant Cell Rep

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Hybrid sterility locus S37 between Oryza glaberrima and Oryza sativa results in both pollen and embryo sac sterility. Interspecific crossing between African cultivated rice Oryza glaberrima and Oryza sativa cultivars is hindered by hybrid sterility. To dissect the mechanism of interspecific hybrid sterility, we developed a near-isogenic line (NIL)-S37 using Dianjingyou1 (DJY1) as the recipient parent and an African cultivated rice variety as the donor parent. Empty pollen and embryo sac sterility were observed in F1 hybrids between DJY1 and NIL-S37. Cytological analyses showed that pollen abortion in the F1 hybrids occurred at the late binucleate stage due to a failure of starch accumulation in pollen grains. In addition, partial abortion of the embryo sac in the F1 hybrid was observed during function megaspore developing into mature embryo sac. Molecular analysis revealed that the semi-sterility was largely caused by the abortion of male and female gametophytes carrying the S37 allele from DJY1. A population of 25,600 plants derived from the hybrid DJY1/NIL-S37 was developed to fine map S37. Based on the physical location of molecular markers, S37 locus was finally delimited to a region of 205 kb on the short arm of chromosome 1 in terms of reference sequences of cv. Nipponbare. Interestingly, an about 97-kb DNA segment was deleted in the NIL-S37 based on BAC clone information of O. glaberrima. Fifty-four open reading frames (ORF) were predicted in this 205-kb region of DJY1, whereas only 31 ORFs were in that of NIL-S37. These results are valuable for cloning of S37 gene and further breaking reproductive isolation between Oryza glaberrima and Oryza sativa cultivars, as well as marker-assisted transferring of the corresponding neutral allele in rice breeding programs.

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Two new loci for hybrid sterility in cultivated rice (Oryza sativa L.)

Cited by 18 publications

References 0 publications

Identification of Indica rice chromosome segments for the improvement of Japonica inbreds and hybrids

Identification of Indica rice chromosome segments for the improvement of Japonica inbreds and hybrids

Fine mapping of locus S-b for F1 pollen sterility in rice (Oryza sativa L.)

Fine mapping of S37, a locus responsible for pollen and embryo sac sterility in hybrids between Oryza sativa L. and O. glaberrima Steud

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