Two New Cases of Polysomy 13 in Canine Prostate Cancer

Abstract

Besides man, the dog is the only known mammalian species that spontaneously develops carcinomas of the prostate with considerable frequency. For this reason, the dog is considered to be the only useful animal model for spontaneously occurring prostate malignancies in man. Cytogenetic investigations of human prostate cancers have revealed the frequent occurrence of trisomies 7, 8, and 17. Chromosome analyses of canine prostate carcinomas are rare. In this report we present 2 cases of canine prostate cancer showing a clonal polysomy 13 along with complex karyotype changes. Along with a previous report demonstrating polysomy 13 as the only karyotype deviation in a canine prostate cancer the present report supports the hypothesis that in canine prostate cancer, polysomy 13 is a recurrent cytogenetic aberration linked to the development of the disease. As human chromosomes (HSA) 8q and 4q and the canine chromosome (CFA) 13 share high homology, these results suggest that a conserved area on these chromosomes is involved in tumorigenesis in both species.

Cytogenetic Analysis of CpG-Oligonucleotide DSP30 plus Interleukin-2-Stimulated Canine B-Cell Lymphoma Cells Reveals the Loss of One X Chromosome as the Sole Abnormality

“…Increasingly, the dog proves to be a promising animal model for the study of human tumor diseases. Recent results of cytogenetic investigations of canine prostate tumors underline the significance of polysomy 13 in the development of prostate cancer [Reimann-Berg et al, 2011]. Canine chromosome 13 shares high homology with human chromosome 8q [Breen et al, 1999].…”

“…Subsequently, the cells were incubated for 20 min in 0.05 M KCl and finally fixed with methanol/glacial acetic acid (3: 1). The suspension was then dropped on ice-cold slides and dried for 5 days at 37 ° C followed by GTG-banding which was performed as previously described [Reimann-Berg et al, 2011]. Results were processed and recorded with BandView, 6.0, MultiSpecies, Applied Spectral Imaging, Israel.…”

Characterization and evaluation of a new oncolytic Vaccinia Virus strain LIVP6.1.1 for canine cancer therapy

“…16 The canine melanoma CHAS cell line was provided by Dr Ogilvie (Angel Care Cancer Center, Carlsbad, USA), and canine osteosarcoma D17 was obtained from ATCC.…”

Virotherapy of Canine Tumors with Oncolytic Vaccinia Virus GLV-1h109 Expressing an Anti-VEGF Single-Chain Antibody

“…In this study, we analyzed the therapeutic potential of the oncolytic vaccinia virus GLV-1h109 strain in two different xenograft models based on canine soft tissue sarcoma STSA-1 cells [4] and canine prostate carcinoma DT08/40 cells [5]. GLV-1h109 virus was derived from the oncolytic vaccinia virus GLV-1h68 [6] by replacing lacZ gene (beta-galactosidase) with GLAF-1 protein encoding gene at J2R locus [7].…”