Two New Ballistoconidium-Forming Yeast Species, Bullera melastomae and Bullera formosana, Found in Taiwan

Molecular phylogeny of basidiomycetous yeasts in theCryptococcus luteoluslineage (Tremellales) based on nuclear rRNA and mitochondrial cytochromebgene sequence analyses: proposal ofDerxomycesgen. nov. andHannaellagen. nov., and description of eight novelDerxomycesspecies

“…Ten more Dioszegia species have been described since then and all of them form orange-coloured colonies with a similar texture (Bai et al, 2002a;Thomas-Hall et al, 2002;Inácio et al, 2005;Wang et al, 2003Wang et al, , 2008, indicating that the morphological characters are stable for the genus. 1) formed pale yellowish to brownish colonies with a butyrous texture (Nakase et al, 2004a), which were somewhat similar to those of some species in the B. mrakii clade. Species in the B. mrakii clade usually form whitish to yellowish colonies with a butyrous to matted texture, while species in the B. sinensis clade usually form whitish colonies with a highly mucoid texture running to the bottom of the slant (see the pictures in the supplementary material).…”

“…A phylogenetic tree was constructed from the 18S rRNA gene sequences, which included almost all currently recognized species in the C. luteolus lineage, the majority of the other Bullera species outside this lineage and representative taxa from other lineages of hymenomycetous yeasts. The two species Bullera formosana and Bullera melastomae described recently formed a separate branch closely related to the Dioszegia clade, as shown in Nakase et al (2004a). The B. mrakii, B. sinensis and Dioszegia clades in this lineage were separated clearly with 100% bootstrap support each.…”

“…The two species B. formosana and B. melastomae that were located in a separate branch in the phylogenetic tree ( Fig. 1) formed pale yellowish to brownish colonies with a butyrous texture (Nakase et al, 2004a), which were somewhat similar to those of some species in the B. mrakii clade.…”

“…The addition of new taxa in the B. mrakii clade reduced the genetic gap between B. anomala and other species in the clade and thus strengthened the inclusion of B. anomala in the clade. The two species Bullera formosana and Bullera melastomae described recently formed a separate branch closely related to the Dioszegia clade, as shown in Nakase et al (2004a). In order to examine the clades in the C. luteolus lineage could be congruently resolved from different genes, a mitochondrial gene was selected for sequencing.…”

“…If the species removed to Udeniomyces and Dioszegia were excluded, only eight species would remain. The development of an improved isolation method of ballistoconidiumforming yeasts (Nakase & Takashima, 1993) and the availability of comprehensive rRNA gene sequence databases of basidiomycetous yeasts (Fell et al, 2000;Scorzetti et al, 2002) have improved the taxonomy of ballistoconidiumforming yeasts and resulted in a remarkable increase in the number of Bullera species described in recent years (Nakase, 2000;Bai et al, 2001aBai et al, , b, 2003Fungsin et al, 2002Fungsin et al, , 2003aFungsin et al, , b, 2006Nakase et al, 2002Nakase et al, , 2004aSampaio et al, 2004;Wang et al, 2004;Luong et al, 2005). A total of 41 species are currently recognized in the genus Bullera.…”