Two new and one known species of Tergestia Stossich, 1899 (Trematoda: Fellodistomidae) with novel molecular characterisation for the genus

Abstract

Combined morphological and molecular analyses are employed to characterise three species of Tergestia Stossich, 1899 (Digenea: Fellodistomidae) from fishes of Moreton Bay, Queensland, Australia. Tergestia clonacantha Manter, 1963 is reported here for the first time from the halfbeak (Beloniformes: Hemiramphidae) species Arrhamphus sclerolepis krefftii (Steindachner), Hyporhamphus australis (Steindachner), H. quoyi (Valenciennes) and H. regularis ardelio (Whitley). Two new species, both infecting trevally (Perciformes: Carangidae) species, are described: T. maryae n. sp. from Alepes apercna Grant and T. henryi n. sp. from Pantolabus radiatus (MacLeay). Complete ITS2 and partial 28S ribosomal DNA data were generated for each of the new taxa. The three species differ from each other by 47-58 base pairs (bp) in the ITS2 rDNA region. Phylogenetic analysis of 28S rDNA supports Tergestia as a reliable generic concept, with our analyses showing that some species of the genus form a well-supported clade to the exclusion of all other fellodistomids for which sequence data are available.

Three new species of Helicometroides Yamaguti, 1934 from Japan and Australia, with new molecular evidence of a widespread species

“…Amplification of the ITS2 and 28S regions was performed as per the protocols of Wee et al . (2017 b ) using the primers 3S (5′-GGT ACC GGT GGA TCA CGT GGC TAG TG-3′; Morgan and Blair, 1995) and ITS2.2 (5′-CCT GGT TAG TTT CTT TTC CTC CGC-3′; Cribb et al ., 1998) for the ITS2 amplification, and LSU5 (5′-TAG GTC GAC CCG CTG AAY TTA AGC A-3′; Littlewood et al ., 2000) and 1500R (5′-GCT ATC CTG AGG GAA ACT TCG-3′; Snyder and Tkach, 2001) or 1200R (5′-GGG CAT CAC AGA CCT G-3′; Lockyer et al ., 2003) for the 28S amplification. Amplification of the 18S region was performed as per the protocols of Martin et al .…”

“…Molecular sequence data were generated for three ribosomal DNA (rDNA) markers: the second internal transcribed spacer region (ITS2), the partial D1-D3 region of the large ribosomal subunit (28S) rDNA coding region and the partial small ribosomal 18S rDNA region; and one mitochondrial DNA (mtDNA) region: the partial cytochrome c oxidase subunit (cox1) region. Amplification of the ITS2 and 28S regions was performed as per the protocols of Wee et al (2017b) using the primers 3S (5 ′ -GGT ACC GGT GGA TCA CGT GGC TAG TG-3 ′ ; Morgan and Blair, 1995) and ITS2.2 (5 ′ -CCT GGT TAG TTT CTT TTC CTC CGC-3 ′ ; Cribb et al, 1998) for the ITS2 amplification, and LSU5 (5 ′ -TAG GTC GAC CCG CTG AAY TTA AGC A-3 ′ ; Littlewood et al, 2000) and 1500R (5 ′ -GCT ATC CTG AGG GAA ACT TCG-3 ′ ; Snyder and Tkach, 2001) or 1200R (5 ′ -GGG CAT CAC AGA CCT G-3 ′ ; Lockyer et al, 2003) for the 28S amplification. Amplification of the 18S region was performed as per the protocols of Martin et al (2017) using the primers WormA (5 ′ -GCG AAT GGC TCA TTA AAT CAG-3 ′ ; Littlewood and Olson, 2001) and WormB (5 ′ -CCT GTT ACG ACT TTT ACT TCC-3 ′ ; Littlewood and Olson, 2001).…”

Gerricola queenslandensis n. g., n. sp., a new monorchiid trematode from the eastern Australian coast and its life cycle partially elucidated

“…Amplification of the two rDNA regions was performed following the protocols of Wee et al . (2017b) using the primers 3S (5′-GGT ACC GGT GGA TCA CGT GGC TAG TG-3′; Morgan & Blair, 1995) and ITS2.2 (5′-CCT GGT TAG TTT CTT TTC CTC CGC-3′; Cribb et al ., 1998) for the ITS2 amplification, and LSU5 (5′-TAG GTC GAC CCG CTG AAY TTA AGC A-3′; Littlewood, 1994) and 1500R (5′-GCT ATC CTG AGG GAA ACT TCG-3′; Snyder & Tkach, 2001) for the 28S amplification. Amplification of the mtDNA region was performed following the protocols of Wee et al .…”

Digenean parasites of deep-sea teleosts: A progress report

“…The other family studied by Bray et al (1999) was the Fellodistomidae, in particular the relationships of members of the genus Steringophorus Odhner, 1905, finding evidence of deep-sea radiation in the genus. Steringophorus has been resolved as monophyletic, usually in a clade with the deep-sea genus Olssonium Bray & Gibson, 1980 and the cold-water genus Fellodistomum Stafford, 1904, in that and most later studies ( Cribb et al, 2014 ; Sun et al, 2014 ; Wee et al, 2017 ). On the other hand, Pérez-Ponce de León et al (2018) added Steringotrema robertpoulini Pérez-Ponce de León, Anglade & Randhawa, 2018 from a shallow water pleuronectid to the phylogeny, finding it a sister species to S. dorsolineatus ( Reimer, 1985 ) from deep sea ipnopids, which together form a clade sister to the remaining members of Steringophorus .…”