Two myostatin genes exhibit divergent and conserved functions in grass carp (Ctenopharyngodon idellus)

Zheng, Guo‐Dong; Sun, Chengfei; Pu, Jian-Wei; Chen, Jie; Jiang, Xia-Yun; Zou, Shu‐Ming

doi:10.1016/j.ygcen.2015.03.008

Cited by 25 publications

(13 citation statements)

References 47 publications

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“…Specific tissue expression of MSTNb can influences endocrine function in many fishes (Kanjanaworakul, Srisapoome, Sawatdichaikul, & Poompuang, ; Morissette et al, ) as well as S. grahami expression pattern in four tissues. Comparative test results reflected that the MSTNb gene was one of the negative factors influencing S. grahami growth, which was in accordance with reports on grass carp (Zheng et al, ) and zebrafish (Lee et al, ; Wang et al, ). Thus, lower gene expression of MSTNb can promote somatic growth in many fishes.…”

Section: Discussionsupporting

confidence: 90%

“…Comparing with mammals (a single encoding MSTN gene), teleost fish possess at least two MSTN genes which are differentially expressed in both muscular and non-muscular tissues to inhibit muscle growth (Rodgers & Weber, 2001 which was in accordance with reports on grass carp (Zheng et al, 2015) and zebrafish (Lee et al, 2009;Wang et al, 2018). Thus, lower gene expression of MSTNb can promote somatic growth in many fishes.…”

Section: Discussionsupporting

confidence: 85%

“…In these four genes, Ghrelin and IGF-I could increase somatic growth by stimulating food intake and food conversion efficiency (Beckman, Larsen, Moriyama, Lee-Pawlak, & Dickhoff, 1998;Unniappan et al, 2002). For myostatin b (MSTNb), a negative regulator in growth of mammals, and also inhibits muscle growth in many fish species (Zheng et al, 2015). In addition, SST1 is known to have a broad spectrum of effects on digestion, energy allocation, and metabolism that negative regulate body growth (Lakeh et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

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Gene expression associated with indicated faster growth of crossbreeding in Sinocyclocheilus grahami

Zhang

Fan²,

Pan

et al. 2019

J Appl Ichthyol

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In this study, pure‐breeding and crossbreeding of Sinocyclocheilus grahami were established to test heterosis and expression of growth‐related genes which were examined in the first‐generation (F1). Genetic distances results showed that Muyang river (M) and Sanbaimu reservoir (S) populations had smaller interspecific distances (genetic distance: 0.000) while larger distances were shown to other two populations (0.024 of Jiuzhai vauclusian spring [J] and 0.044 of Chenglong vauclusian spring [C]). Full‐sib families were created by using the Muyang river population as females: MM, MS, MJ and MC. Growth performance had significant differences between each group of F1 hybrids; the fastest growth group was shown in MJ while the slowest was in MS. For growth‐related genes, the fast growth group of MJ hybrids showed significantly higher levels of ghrelin expression in strong expression tissues of the intestine and liver. Among the four combinations, mRNA levels of insulin‐like growth factor I (IGF‐I) were the highest expression in MJ hybrids than others in all tested tissues. Myostatin b and somatostatin1 (SST1) were strongly expressed in the brain and MJ hybrids showed relatively lower expression level. In the muscle, slow growth group of MS hybrids showed extremely higher expression levels of these two genes, especially SST1 gene. In conclusion, associating growth with growth‐related genes expression indicated faster growth of crossbreeding in S. grahami and can make a contribution to commercial fish production in the future.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionsupporting

confidence: 85%

Section: Introductionmentioning

confidence: 99%

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Gene expression associated with indicated faster growth of crossbreeding in Sinocyclocheilus grahami

Zhang

Fan²,

Pan

et al. 2019

J Appl Ichthyol

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“…Muscle growth is also modulated by myostatin (MSTN), which normally inhibits the growth of skeletal muscle (McPherron et al, 1997 ). Both postnatal aquaculture environment and dietary factors can affect expression levels of MRFs and their related positive & negative regulating genes, as shown in O. mykiss (Alami et al, 2010 ), Piaractus mesopotamicus (Gutierrez et al, 2014 ), O. niloticus (Nebo et al, 2013 ), and C. idellus (Lin et al, 2015 ; Zheng et al, 2015 ). Despite the increasing researches on the relationships between MRFs and their target genes, a comprehensive study on C. idellus is still lacking.…”

Section: Introductionmentioning

confidence: 99%

Diet Affects Muscle Quality and Growth Traits of Grass Carp (Ctenopharyngodon idellus): A Comparison Between Grass and Artificial Feed

et al. 2018

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Fish muscle, the main edible parts with high protein level and low fat level, is consumed worldwide. Diet contributes greatly to fish growth performance and muscle quality. In order to elucidate the correlation between diet and muscle quality, the same batch of juvenile grass carp (Ctenopharyngodon idellus) were divided into two groups and fed with either grass (Lolium perenne, Euphrasia pectinata and Sorghum sudanense) or artificial feed, respectively. However, the different two diets didn't result in significant differences in all the detected water quality parameters (e.g., Tm, pH, DO, NH3/NH4+-N, NO3--N, NO2-, TN, TP, and TOC) between the two experimental groups. After a 4-month culture period, various indexes and expression of myogenic regulatory factor (MRFs) and their related genes were tested. The weight gain of the fish fed with artificial feed (AFG) was nearly 40% higher than the fish fed with grass (GFG). Significantly higher alkaline phosphatase, total cholestrol, high density cholestrol and total protein were detected in GFG as compared to AFG. GFG also showed increased hardness, resilience and shear force in texture profile analysis, with significantly bigger and compact muscle fibers in histologic slices. The fat accumulation was most serious in the abdomen muscle of AFG. Additionally, the expression levels of MyoG, MyoD, IGF-1, and MSTNs were higher, whereas Myf-5, MRF4, and IGF-2 were lower in most positional muscles of GFG as compared to AFG. Overall, these results suggested that feeding grass could promote muscle growth and development by stimulating muscle fiber hypertrophy, as well as significantly enhance the expression of CoL1As. Feeding C. idellus with grass could also improve flesh quality by improving muscle characteristics, enhancing the production of collagen, meanthile, reducing fat accumulation and moisture in muscle, but at the cost of a slower growth.

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“…Mutations of myostatin were also found in other cattle breeds 13 , 14 and mammalian species 15 – 18 . Whereas the mammalian myostatin is exclusively expressed from one gene copy and limited to skeletal muscle 6 , 12 , 19 , two or four copies have been found in fishes 20 – 26 and differentially expressed in many tissues 27 – 36 such as muscle, eye, stomach, skin, brain, gonads, kidney, intestine, liver, spleen, gill and heart. In channel catfish ( Ictalurus punctatus ), the MSTN protein is actively expressed only from one copy on chromosome 6 in different tissues 30 , 37 .…”

Section: Introductionmentioning

confidence: 99%

Generation of Myostatin Gene-Edited Channel Catfish (Ictalurus punctatus) via Zygote Injection of CRISPR/Cas9 System

Khalil

Elayat

Khalifa

et al. 2017

Sci Rep

122

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The myostatin (MSTN) gene is important because of its role in regulation of skeletal muscle growth in all vertebrates. In this study, CRISPR/Cas9 was utilized to successfully target the channel catfish, Ictalurus punctatus, muscle suppressor gene MSTN. CRISPR/Cas9 induced high rates (88–100%) of mutagenesis in the target protein-encoding sites of MSTN. MSTN-edited fry had more muscle cells (p < 0.001) than controls, and the mean body weight of gene-edited fry increased by 29.7%. The nucleic acid alignment of the mutated sequences against the wild-type sequence revealed multiple insertions and deletions. These results demonstrate that CRISPR/Cas9 is a highly efficient tool for editing the channel catfish genome, and opens ways for facilitating channel catfish genetic enhancement and functional genomics. This approach may produce growth-enhanced channel catfish and increase productivity.

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Two myostatin genes exhibit divergent and conserved functions in grass carp (Ctenopharyngodon idellus)

Cited by 25 publications

References 47 publications

Gene expression associated with indicated faster growth of crossbreeding in Sinocyclocheilus grahami

Gene expression associated with indicated faster growth of crossbreeding in Sinocyclocheilus grahami

Diet Affects Muscle Quality and Growth Traits of Grass Carp (Ctenopharyngodon idellus): A Comparison Between Grass and Artificial Feed

Generation of Myostatin Gene-Edited Channel Catfish (Ictalurus punctatus) via Zygote Injection of CRISPR/Cas9 System

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