Two MYB transcription factors (CsMYB2 and CsMYB26) are involved in flavonoid biosynthesis in tea plant [Camellia sinensis (L.) O. Kuntze]

Wang, Wenli; Wang, Yongxin; Li, Hui; Liu, Zhiwei; Cui, Xili; Zhuang, Jing

doi:10.1186/s12870-018-1502-3

Cited by 59 publications

(30 citation statements)

References 39 publications

(48 reference statements)

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“…It has been reported that R2R3 MYB transcription factors act as important regulators in controlling the spatiotemporal biosynthesis of flavonoids during grape and apple fruit development [44,51]. Two MYB transcription factors, CsMYB2 and CsMYB26, were reported to be involved in flavonoid biosynthesis in tea (C. sinensis (L.) O. Kuntze) [52]. Additionally, GmMYB58 and GmMYB205 were demonstrated to be seed-specific activators for isoflavonoid biosynthesis in Glycine max [45].…”

Section: Discussionmentioning

confidence: 99%

Annotation of genes involved in high level of dihydromyricetin production in vine tea (Ampelopsis grossedentata) by transcriptome analysis

Cao

et al. 2020

BMC Plant Biol

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Background: Leaves of the medicinal plant Ampelopsis grossedentata, which is commonly known as vine tea, are used widely in the traditional Chinese beverage in southwest China. The leaves contain a large amount of dihydromyricetin, a compound with various biological activities. However, the transcript profiles involved in its biosynthetic pathway in this plant are unknown. Results: We conducted a transcriptome analysis of both young and old leaves of the vine tea plant using Illumina sequencing. Of the transcriptome datasets, a total of 52.47 million and 47.25 million clean reads were obtained from young and old leaves, respectively. Among 471,658 transcripts and 177,422 genes generated, 7768 differentially expressed genes were identified in leaves at these two stages of development. The phenylpropanoid biosynthetic pathway of vine tea was investigated according to the transcriptome profiling analysis. Most of the genes encoding phenylpropanoid biosynthesis enzymes were identified and found to be differentially expressed in different tissues and leaf stages of vine tea and also greatly contributed to the biosynthesis of dihydromyricetin in vine tea. Conclusions: To the best of our knowledge, this is the first formal study to explore the transcriptome of A. grossedentata. The study provides an insight into the expression patterns and differential distribution of genes related to dihydromyricetin biosynthesis in vine tea. The information may pave the way to metabolically engineering plants with higher flavonoid content.

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Section: Discussionmentioning

confidence: 99%

Annotation of genes involved in high level of dihydromyricetin production in vine tea (Ampelopsis grossedentata) by transcriptome analysis

Cao

et al. 2020

BMC Plant Biol

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“…The CsMYB4a could inhibit the promoter activity of five phenylpropanoid pathway genes (CsANR, CsLAR, CsCHS, Cs4CL, and CsC4H) and two shikimate pathway genes (CsAROC and CsAROF) [92]. Wang et al (2018) confirmed that CsMYB2 and CsMYB26 were involved in flavonoid biosynthesis by regulating the expression of CsF3 H and CsLAR in Camellia sinensis, respectively [93]. Huang et al (2015) cloned and isolated Epimedium sagittatum's two TFs (EsMYBA1 and EsMYBF1) and twelve structural genes.…”

Section: The Role Of Myb Tfs In the Biosynthesis Of Flavonoids Secondmentioning

confidence: 94%

MYB Transcription Factors as Regulators of Secondary Metabolism in Plants

Cao

et al. 2020

Biology

158

103

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MYB transcription factors (TFs), as one of the largest gene families in plants, play important roles in multiple biological processes, such as plant growth and development, cell morphology and pattern building, physiological activity metabolism, primary and secondary metabolic reactions, and responses to environmental stresses. The function of MYB TFs in crops has been widely studied, but few studies have been done on medicinal plants. In this review, we summarized the MYB TFs that play important roles in secondary metabolism and emphasized the possible mechanisms underlying how MYB TFs are regulated at the protein, posttranscriptional, and transcriptional levels, as well as how they regulate the downstream target gene networks related to secondary metabolism in plants, especially in medicinal plants.

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“…Recently, flavonoids in tea (Camellia sinensis) were identified to mediate resistance against a crop destructive disease blister blight caused by Exobasidium vaxans [121]. The flavonoid biosynthesis in tea plants was regulated by CsMYB2/CsMYB26 that was directly bound to the promoters of CsF3'H and CsLAR, two flavonoid biosynthetic genes [104]. These two MYB TFs exhibited correlated inducible gene expression with their target genes upon pathogen infection [104].…”

Section: Myb Tfsmentioning

confidence: 99%

“…The flavonoid biosynthesis in tea plants was regulated by CsMYB2/CsMYB26 that was directly bound to the promoters of CsF3'H and CsLAR, two flavonoid biosynthetic genes [104]. These two MYB TFs exhibited correlated inducible gene expression with their target genes upon pathogen infection [104]. Moreover, three MYB TFs, AtMYB11, AtMYB12, and AtMYB111 were revealed to differentially regulate the flavonoid biosynthesis [97][98][99].…”

Section: Myb Tfsmentioning

confidence: 99%

Transcriptional Factors Regulate Plant Stress Responses Through Mediating Secondary Metabolism

Meraj

Raza

et al. 2020

Genes

164

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Plants are adapted to sense numerous stress stimuli and mount efficient defense responses by directing intricate signaling pathways. They respond to undesirable circumstances to produce stress-inducible phytochemicals that play indispensable roles in plant immunity. Extensive studies have been made to elucidate the underpinnings of defensive molecular mechanisms in various plant species. Transcriptional factors (TFs) are involved in plant defense regulations through acting as mediators by perceiving stress signals and directing downstream defense gene expression. The cross interactions of TFs and stress signaling crosstalk are decisive in determining accumulation of defense metabolites. Here, we collected the major TFs that are efficient in stress responses through regulating secondary metabolism for the direct cessation of stress factors. We focused on six major TF families including AP2/ERF, WRKY, bHLH, bZIP, MYB, and NAC. This review is the compilation of studies where researches were conducted to explore the roles of TFs in stress responses and the contribution of secondary metabolites in combating stress influences. Modulation of these TFs at transcriptional and post-transcriptional levels can facilitate molecular breeding and genetic improvement of crop plants regarding stress sensitivity and response through production of defensive compounds.

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Two MYB transcription factors (CsMYB2 and CsMYB26) are involved in flavonoid biosynthesis in tea plant [Camellia sinensis (L.) O. Kuntze]

Cited by 59 publications

References 39 publications

Annotation of genes involved in high level of dihydromyricetin production in vine tea (Ampelopsis grossedentata) by transcriptome analysis

Annotation of genes involved in high level of dihydromyricetin production in vine tea (Ampelopsis grossedentata) by transcriptome analysis

MYB Transcription Factors as Regulators of Secondary Metabolism in Plants

Transcriptional Factors Regulate Plant Stress Responses Through Mediating Secondary Metabolism

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