Two MP2CL5 Antigen Vaccines from Naegleria fowleri Stimulate the Immune Response against Meningitis in the BALB/c Model

Gutiérrez-Sánchez, Mara; Carrasco-Yépez, María Maricela; Correa‐Basurto, José; Ramírez-Salinas, G. Lizbeth; Rojas-Hernández, Saúl

doi:10.1128/iai.00181-23

Cited by 7 publications

(1 citation statement)

References 86 publications

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“…In addition, Gutiérrez-Sánchez et al 16 research efforts examining immunoprotective responses have identified two peptide-based vaccine antigens promising candidates for preventing meningitis induced by N. fowleri . The immunogenic potential of these antigens is indicated by the substantial protection they provide and the increased levels of IgA, IgG, and IgM in both serum and nasal wash samples 16 . Moreover, the potential therapeutic efficacy of cathepsin B as a target is promising.…”

Section: Introductionmentioning

confidence: 99%

Development and immunological evaluation of an mRNA-based vaccine targeting Naegleria fowleri for the treatment of primary amoebic meningoencephalitis

Naveed,

Ali,

Aziz

et al. 2024

Sci Rep

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More than 95% of patients fall victim to primary amoebic meningoencephalitis (PAM), a fatal disease attacking the central nervous system. Naegleria fowleri, a brain-eating microorganism, is PAM's most well-known pathogenic ameboflagellate. Despite the use of antibiotics, the fatality rate continues to rise as no clinical trials have been conducted against this disease. To address this, we mined the UniProt database for pathogenic proteins and selected assumed epitopes to create an mRNA-based vaccine. We identified thirty B-cell and T-cell epitopes for the vaccine candidate. These epitopes, secretion boosters, subcellular trafficking structures, and linkers were used to construct the vaccine candidate. Through predictive modeling and confirmation via the Ramachandran plot (with a quality factor of 92.22), we assessed secondary and 3D structures. The adjuvant RpfE was incorporated to enhance the vaccine construct's immunogenicity (GRAVY index: 0.394, instability index: 38.99, antigenicity: 0.8). The theoretical model of immunological simulations indicated favorable responses from both innate and adaptive immune cells, with memory cells expected to remain active for up to 350 days post-vaccination, while the antigen was eliminated from the body within 24 h. Notably, strong interactions were observed between the vaccine construct and TLR-4 (− 11.9 kcal/mol) and TLR-3 (− 18.2 kcal/mol).

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Section: Introductionmentioning

confidence: 99%

Development and immunological evaluation of an mRNA-based vaccine targeting Naegleria fowleri for the treatment of primary amoebic meningoencephalitis

Naveed,

Ali,

Aziz

et al. 2024

Sci Rep

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Immunogens in Balamuthia mandrillaris: a proteomic exploration

Alfaro-Sifuentes,

Lares-Jiménez,

Rojas-Hernández

et al. 2024

Parasitol Res

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The Pathology of the Brain Eating Amoeba Naegleria fowleri

Borkens

2024

Indian J Microbiol

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The genus Naegleria is a taxonomic subfamily consisting of 47 free-living amoebae. The genus can be found in warm aqueous or soil habitats worldwide. The species Naegleria fowleri is probably the best-known species of this genus. As a facultative parasite, the protist is not dependent on hosts to complete its life cycle. However, it can infect humans by entering the nose during water contact, such as swimming, and travel along the olfactory nerve to the brain. There it causes a purulent meningitis (primary amoebic meningoencephalitis or PAME). Symptoms are severe and death usually occurs within the first week. PAME is a frightening infectious disease for which there is neither a proven cure nor a vaccine. In order to contain the disease and give patients any chance to survival, action must be taken quickly. A rapid diagnosis is therefore crucial. PAME is diagnosed by the detection of amoebae in the liquor and later in the cerebrospinal fluid. For this purpose, CSF samples are cultured and stained and finally examined microscopically. Molecular techniques such as PCR or ELISA support the microscopic analysis and secure the diagnosis.

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Two MP2CL5 Antigen Vaccines from Naegleria fowleri Stimulate the Immune Response against Meningitis in the BALB/c Model

Cited by 7 publications

References 86 publications

Development and immunological evaluation of an mRNA-based vaccine targeting Naegleria fowleri for the treatment of primary amoebic meningoencephalitis

Development and immunological evaluation of an mRNA-based vaccine targeting Naegleria fowleri for the treatment of primary amoebic meningoencephalitis

Immunogens in Balamuthia mandrillaris: a proteomic exploration

The Pathology of the Brain Eating Amoeba Naegleria fowleri

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