Two-month ciprofloxacin implants for multibacterial bone infections

Castro, Carlos; Évora, Carmen; Baro, Manuel; Soriano, I.; Sánchez, Esther

doi:10.1016/j.ejpb.2005.02.005

Cited by 38 publications

(23 citation statements)

References 9 publications

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“…Right femurs were extracted, three of them cut longitudinally and the CFX implants removed. CFX remaining in the implants was totally extracted by incubation in citrate buffer pH 4.0 on a shaker and then assayed microbiologically with the agar diffusion method using Bacillus subtilis (ATCC 6051) 7, 8, 35. Bacterial concentration in femurs was determined at each time point.…”

Section: Methodsmentioning

confidence: 99%

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Efficacy of ciprofloxacin implants in treating experimental osteomyelitis

et al. 2007

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Ciprofloxacin (CFX) implants containing poly(D,L-lactide) and calcium phosphates (tricalcium phosphate and hydroxyapatite) was evaluated in 50 rabbits in an experimental osteomyelitis model. Their femoral cavity was inoculated with Staphylococcus aureus. After 2 weeks, the infected focus was cleaned out and the delivery system implanted. The infection and subsequent response to treatment were evaluated by microbiological analysis, biochemical and hematological markers, body weight, temperature, clinical signs, X-rays, and histology. Infected bone cultures, treated with CFX implants, showed reduced bacterial growth against controls. All CFX was released within 6 weeks. All animals recovered within 4 weeks. Even 12 weeks after implantation, no recurrence of infection was observed. Serum C-reactive protein, platelet, and leukocyte levels increased in all animals before treatment, and 4 weeks after it were maintained or rose in control animals, while decreased to normal levels in treated ones. Body weight was characterized by pretreatment losses, then gains during recuperation, or further loss in untreated animals; with no significant intraindividual differences in body temperature. Body weight, leucocytes, platelets, and C-reactive protein turned out to be highly useful markers for monitoring this kind of infection and its treatment. CFX implants demonstrated to be an effective therapy for S. aureus bone infection. Their efficacy was also reflected in decreasing severity of clinical signs, nonprogress of radiological signs indicative of infection, and good integration into bone structure. Histological examination revealed repair, with new bone formation extending into implants.

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Section: Methodsmentioning

confidence: 99%

“…The osteoconductive component of the system may guide healing of the bone defect. Such biodegradable local release systems for CFX have already been prepared by our research group and characterized in vitro and in vivo , testing the antibiotic levels remaining in bone 7, 8…”

Section: Introductionmentioning

confidence: 99%

Efficacy of ciprofloxacin implants in treating experimental osteomyelitis

et al. 2007

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“…Although several novel techniques have been developed to introduce therapeutic drugs within scaffolds, in most cases the strategy followed has been the direct incorporation of the drug into the scaffold by immersion of the scaffold in a drug-containing buffer aqueous solution. Nevertheless, thermo-labile drugs can also be loaded within threedimensional scaffolds in a one-step process using room temperature compaction of the powder mix, this being a solvent-free process, which avoids the use of toxic solvents (Kimakhe et al 1999;Vallet-Regí et al 2001;Castro et al 2005). Further details of relevant studies are discussed in the following sections, highlighting the strengths and weaknesses of the different approaches.…”

Section: Processing Techniquesmentioning

confidence: 99%

“…CFX has been considered in combination with bioceramics and polymers. For example CFX was loaded into a composite comprising HA, TCP and poly(DL-lactide) (PLA) to treat complicated multi-organism bone infections that required high antibiotic concentrations (40%) in bone for long periods (Castro et al 2005). The composites were prepared by mixing the ingredients and by further uniaxial compression using a hydraulic press.…”

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confidence: 99%

Bone tissue engineering therapeutics: controlled drug delivery in three-dimensional scaffolds

Mouriño

Boccaccını

2009

J. R. Soc. Interface.

472

298

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This paper provides an extensive overview of published studies on the development and applications of three-dimensional bone tissue engineering (TE) scaffolds with potential capability for the controlled delivery of therapeutic drugs. Typical drugs considered include gentamicin and other antibiotics generally used to combat osteomyelitis, as well as anti-inflammatory drugs and bisphosphonates, but delivery of growth factors is not covered in this review. In each case reviewed, special attention has been given to the technology used for controlling the release of the loaded drugs. The possibility of designing multifunctional three-dimensional bone TE scaffolds for the emerging field of bone TE therapeutics is discussed. A detailed summary of drugs included in three-dimensional scaffolds and the several approaches developed to combine bioceramics with various polymeric biomaterials in composites for drug-delivery systems is included. The main results presented in the literature are discussed and the remaining challenges in the field are summarized with suggestions for future research directions.

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“…2 Among the several antibiotics, ciprooxacin is much preferred for the treatment of osteomyelitis since its minimal inhibitory concentration (MIC) is low (0.25-2 mg ml À1 ) for most of the pathogens that cause osteomyelitis. [27][28][29] The aim of the present work is to synthesize HA nanoparticles having the potential to deliver ciprooxacin in a sustained and prolonged manner. …”

Section: Introductionmentioning

confidence: 99%

In situ synthesis, characterization and in vitro studies of ciprofloxacin loaded hydroxyapatite nanoparticles for the treatment of osteomyelitis

2014

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Bioactive materials in combination with antibiotics are widely developed for the treatment of osteomyelitis which play a dual role: as bone substitutes at the affected site and as local drug delivery systems for antibiotic delivery. In the present study, a series of ciprofloxacin loaded hydroxyapatite (HA) nanoparticles with sustained and prolonged release behavior has been synthesized by an in situ precipitation method. The amount of ciprofloxacin loaded on HA nanoparticles can be easily adjusted by changing the initial concentration during the synthesis. It was observed that as the loaded concentration of ciprofloxacin increases the release is sustained and prolonged. The in situ loading of ciprofloxacin onto HA nanoparticles does not significantly affect the bioactivity and cytocompatibility of HA whereas it provides antibacterial activity to HA against S. aureus and E. coli that causes osteomyelitis. Consequently synthesized ciprofloxacin loaded HA nanoparticles can be potential candidates for the treatment of osteomyelitis.

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Two-month ciprofloxacin implants for multibacterial bone infections

Cited by 38 publications

References 9 publications

Efficacy of ciprofloxacin implants in treating experimental osteomyelitis

Efficacy of ciprofloxacin implants in treating experimental osteomyelitis

Bone tissue engineering therapeutics: controlled drug delivery in three-dimensional scaffolds

In situ synthesis, characterization and in vitro studies of ciprofloxacin loaded hydroxyapatite nanoparticles for the treatment of osteomyelitis

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