Two Monoclonal Antibodies Recognizing Determinants on Human Embryonal Carcinoma Cells React Specifically with the Liver Isozyme of Human Alkaline Phosphatase

Abstract

From a series of hybridomas that produced monoclonal antibodies reactive with the surface of human embryonal carcinoma cells, two that specifically recognized determinants of the liver/bone/kidney isozyme of alkaline phosphatase were isolated. They did not cross-react with the intestinal or placental isozymes. Phylogenetic studies revealed that both antibodies cross-reacted strongly with liver alkaline phosphatase from higher primates, but exhibited marked differences in their respective cross-reactions with liver alkaline phosphatase from other mammalian species.

Comparative analysis of cell surface antigens expressed by cell lines derived from human germ cell tumours

“…VINIS56 and VIN2PB22 reacted strongly with RA-induced cells, and also with HMBA-induced cells after prolonged periods of differentiation. These results accord with previously published observations (Andrews, 1984;Andrews et al, 1990).…”

“…Differentiated cultures of the pluripotent EC cell line NTERA-2 clone D1 (NT2/D1) were produced by exposure for 7, 14 and 21 days to M all-trans retinoic acid (RA) or 3 mM hexamethylene bisacetamide (HMBA), as previously described (Andrews, 1984;Andrews et al, 1990).…”

“…Inspection of the data shown in Table I1 reveals that the NT2/D1 cells exhibited rather more variability in expression of antigens thought typical of EC cells (e.g., those detected by antibodies MC631, MC813-70 and TRA-1-60; see below) than did the 2102Ep 4D3 cells. This finding most likely relates to the ability of NT2/D1 cells to undergo extensive differentiation (Andrews et al, 1984b;Andrews, 1984), and so may reflect variable amounts of partial spontaneous differentiation in NT2/D1 cultures, in comparison with cultures of 2102Ep 4D3 cells which possess little capacity for differentiation (Andrews et al, 1982). …”

The Wistar Legacy to Embryonic Stem Cell Research

“…Andrews continued to define the human embryonic stem cells on the EC tumor-derived cultures by producing new hybridomas secreting antibodies that recognized epitopes of sialylated keratan sulfate (TRA 1-60, TRA 1-81), (23) and other hybridomas that produced antibodies reactive with the form of alkaline phosphatase previously know to mark mouse and human embryonic stem cells. (24) Again we produced a series of high-affinity monoclonal antibodies, extremely useful markers of human tumor-derived cell lines and early embryonic cells, but the carbohydrate epitopes themselves did not appear to be of immediate functional significance.…”

Identificação de vias moduladas por microRNAs na diferenciação celular e manutenção da pluripotência em células humanas

“…Essa similaridade faz das CCE bons modelos experimentais para estudos in vitro sobre a pluripotência e a diferenciação celular, uma vez que o cultivo de CTE, principalmente das humanas, apresentam um elevado custo e complexidade (ANDREWS et al, 1982(ANDREWS et al, , 1984a(ANDREWS et al, , 1984bBADCOCK et al, 1999;DRAPER et al, 2002;GREBER;LEHRACH;ADJAYE, 2007;STEWART et al, 2008).…”