Two Modes of Autoregulation of the<i>murR</i>Repressor in<i>Escherichia coli</i>

Ishihama, Akira

doi:10.1271/bbb.90421

Cited by 5 publications

(3 citation statements)

References 8 publications

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“…To measure the activity and regulation of promoters, a LacZ-reporter assay was employed using pRS551 plasmid vector [35]. Promoter fragments of approximately 500 bp in length between the initiation codon and 500 bp upstream sequence were amplified by PCR using a pair of primers (F and R) (for sequences see Table S1).…”

Section: Methodsmentioning

confidence: 99%

Novel Roles of cAMP Receptor Protein (CRP) in Regulation of Transport and Metabolism of Carbon Sources

et al. 2011

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CRP (cAMP receptor protein), the global regulator of genes for carbon source utilization in the absence of glucose, is the best-studied prokaryotic transcription factor. A total of 195 target promoters on the Escherichia coli genome have been proposed to be under the control of cAMP-bound CRP. Using the newly developed Genomic SELEX screening system of transcription factor-binding sequences, however, we have identified a total of at least 254 CRP-binding sites. Based on their location on the E. coli genome, we predict a total of at least 183 novel regulation target operons, altogether with the 195 hitherto known targets, reaching to the minimum of 378 promoters as the regulation targets of cAMP-CRP. All the promoters selected from the newly identified targets and examined by using the lacZ reporter assay were found to be under the control of CRP, indicating that the Genomic SELEX screening allowed to identify the CRP targets with high accuracy. Based on the functions of novel target genes, we conclude that CRP plays a key regulatory role in the whole processes from the selective transport of carbon sources, the glycolysis-gluconeogenesis switching to the metabolisms downstream of glycolysis, including tricarboxylic acid (TCA) cycle, pyruvate dehydrogenase (PDH) pathway and aerobic respiration. One unique regulation mode is that a single and the same CRP molecule bound within intergenic regions often regulates both of divergently transcribed operons.

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Section: Methodsmentioning

confidence: 99%

Novel Roles of cAMP Receptor Protein (CRP) in Regulation of Transport and Metabolism of Carbon Sources

et al. 2011

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“…To measure the activity and regulation of promoters, a LacZ reporter assay was employed by using plasmid vector pRS551 (20). Promoter fragments between position Ϫ500 and the start codon were amplified by PCR using a pair of primers (F and R) (for sequences, see Table S1 in the supplemental material).…”

Section: Methodsmentioning

confidence: 99%

Novel Members of the Cra Regulon Involved in Carbon Metabolism in Escherichia coli

2011

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Cra (catabolite repressor activator) is a global regulator of the genes for carbon metabolism in Escherichia coli. To gain insights into the regulatory roles of Cra, attempts were made to identify the whole set of regulation targets using an improved genomic SELEX (systematic evolution of ligands by exponential enrichment) system. Surprisingly, a total of 164 binding sites were identified for Cra, 144 (88%) of which were newly identified. The majority of known targets were included in the SELEX chip pattern. The promoters examined by the lacZ reporter assay in vivo were all regulated by Cra. These two lines of evidence indicate that a total of as many as 178 promoters are under the control of Cra. The majority of Cra targets are the genes coding for the enzymes involved in central carbon metabolism, covering all the genes for the enzymes involved in glycolysis and metabolism downstream of glycolysis, including the tricarboxylic acid (TCA) cycle and aerobic respiration. Taken together, we propose that Cra plays a key role in balancing the levels of the enzymes for carbon metabolism.Central carbon metabolism consists of three major pathways, the glycolysis (Embden-Meyerhof-Parnas [EMP]) pathway, the pentose-phosphate (PP) pathway, and the tricarboxylic acid (TCA) cycle, altogether leading to the production of metabolic energy. These three pathways also provide a number of building blocks that are utilized for the construction of cell architecture (for reviews, see references 26, 43, and 50).

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“…SbcB mutation of E. coli could suppress the cell deficiency in postreplication repair by enabling the genes uvrA , recB , and recC to repair DNA double-strand breaks [ 54 ], suggesting that down-regulation of sbcB might be helpful for cells to repair the damaged DNA caused by inhibitory stresses. MurR is a putative transcription factor that could repress the murPQ operon, which is required for the cellular catabolism of the bacterial cell wall [ 55 ]. Also, Ea22, probably derived from bacteriophages, is an uncharacterized protein involved in the cell cycle control, cell division, and chromosome partitioning of E. coli [ 56 ].…”

Section: Discussionmentioning

confidence: 99%

Inhibitor tolerance and bioethanol fermentability of levoglucosan-utilizing Escherichia coli were enhanced by overexpression of stress-responsive gene ycfR: The proteomics-guided metabolic engineering

Chang

Islam

Zheng

et al. 2021

Synthetic and Systems Biotechnology

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Two Modes of Autoregulation of themurRRepressor inEscherichia coli

Cited by 5 publications

References 8 publications

Novel Roles of cAMP Receptor Protein (CRP) in Regulation of Transport and Metabolism of Carbon Sources

Novel Roles of cAMP Receptor Protein (CRP) in Regulation of Transport and Metabolism of Carbon Sources

Novel Members of the Cra Regulon Involved in Carbon Metabolism in Escherichia coli

Inhibitor tolerance and bioethanol fermentability of levoglucosan-utilizing Escherichia coli were enhanced by overexpression of stress-responsive gene ycfR: The proteomics-guided metabolic engineering

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