Two Methods of Preparing Washed and/or Replaced Platelet Concentrates

Akino, Mitsuaki; Tamura, Shinji; Hirayama, Junichi; Naito, Yoshiro; Katsumata, Masako; Homma, Chihiro; Yamamoto, Shozo; Fujihara, Mitsuhiro; Azuma, Hiroshi; Kato, Toshiaki; Ikeda, Hisami

doi:10.3925/jjtc.55.698

Cited by 5 publications

(2 citation statements)

References 16 publications

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“…Usually, the fluorescent intensity of CD62P was weak, and not distinctly isolated from negative fraction. We prepared the samples for the analysis of CD62P expression on platelet using previously reported protocol [9]. The rate of CD62P expression on platelets in their report was 1Á9 AE 0Á8%.…”

Section: Discussionmentioning

confidence: 99%

“…To identify activated platelets, anti-CD61 monoclonal antibody (clone P2, mAb) conjugated with fluorescein isothiocyanate (clone P2FITC, Beckman Coulter, Brea, CA) and anti-CD62P mAb (clone CLBTromb/6) conjugated with phycoerythrin (PE, Beckman Coulter) were used in this study as previously described [ [7][8][9]]. In brief, 50 ll of samples taken from PC bag at each point was suspended in 1000 ll of phosphate-buffered saline (PBS) with 1% paraformaldehyde for fixation and incubated at the platelet count of 1Á25x10 4 /ll.…”

Section: Parameters For Blood Product Qualitymentioning

confidence: 99%

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Quality of red blood cell and platelet concentrates after transportation by a pneumatic tube system

Kobayashi

Ebihara

Asami

et al. 2019

ISBT Science Series

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Background and objectives Pneumatic tube systems (PTSs) are used in some hospitals to transport patients' specimens. We investigated the feasibility of applying PTS for blood products for transfusion in our hospital. Materials and methodsWe transported red blood cells (RBCs) and platelet concentrates (PCs) by the PTS and measured biomarkers such as red cell haemolysis and platelet dysfunction before and after one-way transportation, in addition, after one-way transportation for three consecutive times by PTS.Results Using 25 RBCs, K, free Hb, Hb, LD, AST and pH were compared before and after transportation. There were no significant differences. Haemolysis rates ranging between 0Á13% and 0Á35% were acceptable under the guideline. Using 6 PCs, platelet concentration, aggregation and activation function were examined. There were no significant differences before and after transportation. Positive rates of CD62P ranging between 3Á1% and 11Á4% showed no remarkable changes after the transportation. After the third consecutive transportation, LD and AST were slightly increased. However, haemolysis rate changed from 0Á16% to 0Á21%, again stayed in guideline level. As for platelet products, platelet aggregation activities to collagen (approximately 80%) did not change significantly. ConclusionOur study demonstrated that PTS is feasible for transporting them since one-and three-time transportation by PTS does not appear to influence the important quality of RBCs and PCs. In our hospital, we have applied PTS for the transportation of blood products without major troubles such as loss of products or transfusion-related adverse events.

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Section: Discussionmentioning

confidence: 99%

Section: Parameters For Blood Product Qualitymentioning

confidence: 99%