Two-method verification of hydrogen peroxide determinations in natural waters

Kieber, Robert J.; Helz, George R.

doi:10.1021/ac00124a043

Cited by 101 publications

(63 citation statements)

References 23 publications

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“…Fluorometric H,O, analysis-Peroxide was analyzed fluorometrically using scopoletin (7-hydroxy-6-methoxy-2H-benzopyran) as a fluorescence indicator (Andreae 1955) in a peroxidase-catalyzed reaction (Kieber and Helz 1986;Cooper et al 1989). A Kontron SFM 25 fluorometer was used at 365-nm excitation and 490-nm emission wavelengths.…”

Section: Methodsmentioning

confidence: 99%

Dynamics of UV‐driven hydrogen peroxide formation on an intertidal sandflat

Abele

Tüg

Röttgers

1997

Limnology & Oceanography

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Photochemically produced H,O, was found to accumulate at micromolar concentrations in intertidal Waddcn Sea areas. Annual amplitudes of solar radiation lead to variations of the intertidal H,O, accumulation with concentrations between 1 and 4 p,mol liter 1 during summer, while winter concentrations were mostly CO.5 pmol liter-l. Diurnal variations of H,O, accumulation over daily low-tide periods in intertidal environments are determined by the incident solar radiation, the concentration of UV-absorbing dissolved organic carbon in the water, as well as the concomitant biological and chemical H,O, degradation within the sediment surface. The efficiency of photosynthetic available radiation (PAR), UVA and UVB photons for photochemical H,O, production was assessed using cut-off filters in the UVB and in different UVA ranges. UVB photons (295-320 nm) displayed an 1 i-fold higher efficiency compared to UVA (335-370 nm) and a 340-fold higher efficiency compared to PAR photons (>400 nm). A 10% ozone reduction leads to a doubling of UVB surface irradiance at 300 nm, which entails a 30 and 40% increase of the apparent intertidal H,O, concentrations. Progressive stratospheric ozone depletion via UVB-induced H,O, formation will have yet unpredictable effects on boreal and Antarctic intertidal ecosystems.

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Section: Methodsmentioning

confidence: 99%

Dynamics of UV‐driven hydrogen peroxide formation on an intertidal sandflat

Abele

Tüg

Röttgers

1997

Limnology & Oceanography

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“…Supporting measurements of total H,O, were made at each time interval in the above experiments with the HRP scopoletin technique described elsewhere (Kieber and Helz 1986).…”

Section: Methodsmentioning

confidence: 99%

An investigation ofhydrogen peroxide chemistry in surface waters of Vineyard Sound with H218O2 and 18O2

Moffett¹,

Zajiriou²

1990

Limnology & Oceanography

120

102

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“…Hydrogen peroxide (H 2 O 2 ) was analyzed using a fluorescence decay technique involving the peroxidase-mediated oxidation of the fluorophore scopoletin by H 2 O 2 in rain and buffered at a pH of 7 with a phosphate buffer (Kieber and Helz, 1986). Fluorescence measurements were made on a Turner Designs (Sunnyvale, CA) Model 7200-000 fluorometer equipped with a H 2 O 2 module wavelength filter (λ ex = 365 ± 10 nm, λ em = 486 ± 10 nm).…”

Section: Hydrogen Peroxidementioning

confidence: 99%

“…H 2 O 2 standard solutions were prepared fresh from a stock solution of 0.1 M H 2 O 2 , which had been standardized by titration with phenylarsine oxide. The method has an analytical precision of 2 % relative standard deviation (% RSD) at ambient rainwater concentrations (Kieber and Helz, 1986). All samples were analyzed in triplicate for H 2 O 2 .…”

Section: Hydrogen Peroxidementioning

confidence: 99%

Dynamics of the chemical composition of rainwater throughout Hurricane Irene

et al. 2013

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Abstract. Sequential sampling of rainwater from Hurricane Irene was carried out in Wilmington, NC, USA on 26 and 27 August 2011. Eleven samples were analyzed for pH, major ions (Cl−, NO3−, SO42−, Na+, K+, Mg2+, Ca2+, NH4+), dissolved organic carbon (DOC) and hydrogen peroxide (H2O2). Hurricane Irene contributed 16% of the total rainwater and 18% of the total chloride wet deposition received in Wilmington NC during all of 2011. This work highlights the main physical factors influencing the chemical composition of tropical storm rainwater: wind speed, wind direction, back trajectory and vertical mixing, time of day and total rain volume. Samples collected early in the storm, when winds blew out of the east, contained dissolved components indicative of marine sources (salts from sea spray and low DOC). The sea-salt components in the samples had two maxima in concentration during the storm the first of which occurred before the volume of rain had sufficiently washed out sea salt from the atmosphere and the second when back trajectories showed large volumes of marine surface air were lifted. As the storm progressed and winds shifted to a westerly direction, the chemical composition of the rainwater became characteristic of terrestrial storms (high DOC and NH4+ and low sea salt). This work demonstrates that tropical storms are not only responsible for significant wet deposition of marine components to land, but terrestrial components can also become entrained in rainwater, which can then be delivered to coastal waters via wet deposition. This study also underscores why analysis of one composite sample can lead to an incomplete interpretation of the factors that influence the chemically divergent analytes in rainwater during extreme weather events.

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Two-method verification of hydrogen peroxide determinations in natural waters

Cited by 101 publications

References 23 publications

Dynamics of UV‐driven hydrogen peroxide formation on an intertidal sandflat

Dynamics of UV‐driven hydrogen peroxide formation on an intertidal sandflat

An investigation ofhydrogen peroxide chemistry in surface waters of Vineyard Sound with H218O2 and 18O2

Dynamics of the chemical composition of rainwater throughout Hurricane Irene

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