Two members of unassigned type of short-chain dehydrogenase/reductase superfamily (SDR) isolated from Persicaria minor show response towards ABA and drought stress

Hamid, Nur Athirah Abd; Zainal, Zamri; Ismail, Ismanizan

doi:10.1007/s13562-017-0436-4

Cited by 4 publications

(4 citation statements)

References 44 publications

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“…ABA is involved in the response to abiotic stresses [ 275 ]. In drought, dsm2 mutants have shown impaired β-carotene hydroxylase synthesis in rice [ 85 ].…”

Section: Genes Regulating Ros Homeostasis In Abiotic Stressmentioning

confidence: 99%

ROS Homeostasis in Abiotic Stress Tolerance in Plants

Nadarajah

2020

IJMS

373

181

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Climate change-induced abiotic stress results in crop yield and production losses. These stresses result in changes at the physiological and molecular level that affect the development and growth of the plant. Reactive oxygen species (ROS) is formed at high levels due to abiotic stress within different organelles, leading to cellular damage. Plants have evolved mechanisms to control the production and scavenging of ROS through enzymatic and non-enzymatic antioxidative processes. However, ROS has a dual function in abiotic stresses where, at high levels, they are toxic to cells while the same molecule can function as a signal transducer that activates a local and systemic plant defense response against stress. The effects, perception, signaling, and activation of ROS and their antioxidative responses are elaborated in this review. This review aims to provide a purview of processes involved in ROS homeostasis in plants and to identify genes that are triggered in response to abiotic-induced oxidative stress. This review articulates the importance of these genes and pathways in understanding the mechanism of resistance in plants and the importance of this information in breeding and genetically developing crops for resistance against abiotic stress in plants.

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“…ABA is involved in the response to abiotic stresses [ 275 ]. In drought, dsm2 mutants have shown impaired β-carotene hydroxylase synthesis in rice [ 85 ].…”

Section: Genes Regulating Ros Homeostasis In Abiotic Stressmentioning

confidence: 99%

ROS Homeostasis in Abiotic Stress Tolerance in Plants

Nadarajah

2020

IJMS

373

181

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“…Analysis of the SDRc and SDRd knockdown mutant lines suggested that these two SDRs are responsible for benzaldehyde biosynthesis in Arabidopsis and exhibited strict substrate specificity towards benzoyl-CoA, similar to their homologues PhBSb and PhBSa from Petunia hybrida (Huang et al, 2022). Although it does not have apparent homologs in the moss Physcomitrella patens and the gymnosperm Ginkgo biloba, SDRc is grouped with several PTS1-containing SDRs from higher plants, including CmADH2 from Cucumis melo (Manrıquez et al, 2006;Jin et al, 2016), PmADHa and PmADHb from Persicaria minor (Hamid et al, 2018), and PhBSb from Petunia (Huang et al, 2022) (Figure 2). CmADH2 a short-chain alcohol dehydrogenase with higher activity in alcohol reduction than oxidation and strong substrate specificity towards acetaldehyde, and was predicted to use NADH as a co-factor (Manrıquez et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

“…CmADH2 a short-chain alcohol dehydrogenase with higher activity in alcohol reduction than oxidation and strong substrate specificity towards acetaldehyde, and was predicted to use NADH as a co-factor (Manrıquez et al, 2006). The expression of PmADHa and PmADHb are up-regulated by ABA treatment and drought stress, suggesting their probable role in ABA signaling and drought response (Hamid et al, 2018). PhBSb is a subunit of a heterodimeric enzyme PhBS, a benzaldehyde synthase with strict substrate selectivity for benzoyl-CoA (Huang et al, 2022).…”

Section: Discussionmentioning

confidence: 99%

Bioinformatic analysis of short-chain dehydrogenase/reductase proteins in plant peroxisomes

Zhang

Wang

et al. 2023

Front. Plant Sci.

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Peroxisomes are ubiquitous eukaryotic organelles housing not only many important oxidative metabolic reactions, but also some reductive reactions that are less known. Members of the short-chain dehydrogenase/reductase (SDR) superfamily, which are NAD(P)(H)-dependent oxidoreductases, play important roles in plant peroxisomes, including the conversion of indole-3-butyric acid (IBA) to indole-3-acetic acid (IAA), auxiliary β-oxidation of fatty acids, and benzaldehyde production. To further explore the function of this family of proteins in the plant peroxisome, we performed an in silico search for peroxisomal SDR proteins from Arabidopsis based on the presence of peroxisome targeting signal peptides. A total of 11 proteins were discovered, among which four were experimentally confirmed to be peroxisomal in this study. Phylogenetic analyses showed the presence of peroxisomal SDR proteins in diverse plant species, indicating the functional conservation of this protein family in peroxisomal metabolism. Knowledge about the known peroxisomal SDRs from other species also allowed us to predict the function of plant SDR proteins within the same subgroup. Furthermore, in silico gene expression profiling revealed strong expression of most SDR genes in floral tissues and during seed germination, suggesting their involvement in reproduction and seed development. Finally, we explored the function of SDRj, a member of a novel subgroup of peroxisomal SDR proteins, by generating and analyzing CRISPR/Cas mutant lines. This work provides a foundation for future research on the biological activities of peroxisomal SDRs to fully understand the redox control of peroxisome functions.

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“…Two SDRs PmADHa and PmADHb were conducted under the exogenous ABA treatment and drought stress in leaves, stems and roots. Exogenous ABA treatment showed that the high expression of PmADHs was tested only in roots, whereas the expression of PmADHa and PmADHb were upregulated in response to drought stress in all organs (Hamid et al, 2018). The expression of SDR1 (unique SDR) showed the dynamic mobilisation of ABA precursors and/or ABA in Arabidopsis (Cheng et al, 2002).…”

Section: The Substrate Catalysis Characterisation Of the Purified Recmentioning

confidence: 99%

Differential expression and functional analysis of two short-chain alcohol dehydrogenases/reductases in Hedychium coronarium

Chen

Yue

et al. 2020

Folia Horticulturae

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In this study, the full cDNA sequences of HcADH2 and HcADH3 were cloned from Hedychium coronarium. The amino acid sequences encoded by them contained three most conserved motifs of short-chain alcohol dehydrogenase (ADH), namely NAD+ binding domain, TGxxx[AG]xG and active site YxxxK. The highest similarity between two genes and ADH from other plants was 70%. Phylogenetic analysis showed that they belonged to a member of the short-chain dehydrogenases/reductases 110C subfamily, but they were distinctly clustered in different clades. Real-time polymerase chain reaction analyses showed that HcADH2 was specifically expressed in bract, and it was expressed higher in no-scented Hedychium forrestii than other Hedychium species, but was undetectable in Hedychium coccineum. HcADH3 was expressed higher in the lateral petal of the flower than in other vegetative organs, and it was expressed the most in H. coronarium that is the most scented among Hedychium species, and its expression levels peaked at the half opening stage. HcADH2 and HcADH3 had almost no significant expression in leaves, but HcADH2 was expressed in response to external stimuli. The mechanical injury and methyl jasmonate (MeJA) treatment could induce expression of HcADH2 in leaves, whereas HcADH3 could have an induced expression only by MeJA. The recombinant HcADH3 protein, but not HcADH2, expressed in Escherichia coli-catalysed conversion of geraniol into citral. It was speculated that HcADH3 had an induced expression in vegetative organ of H. coronarium and took part in monoterpenoid biosynthesis in H. coronarium flowers, but the role of HcADH2 is relevant only for defensive reactions.

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Two members of unassigned type of short-chain dehydrogenase/reductase superfamily (SDR) isolated from Persicaria minor show response towards ABA and drought stress

Cited by 4 publications

References 44 publications

ROS Homeostasis in Abiotic Stress Tolerance in Plants

ROS Homeostasis in Abiotic Stress Tolerance in Plants

Bioinformatic analysis of short-chain dehydrogenase/reductase proteins in plant peroxisomes

Differential expression and functional analysis of two short-chain alcohol dehydrogenases/reductases in Hedychium coronarium

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