Two Members of the Tcf Family Implicated in Wnt/β-Catenin Signaling during Embryogenesis in the Mouse

Kor̆ínek, Vladimír; Barker, Nick; Willert, Karl; Molenaar, Miranda; Roose, Jeroen P.; Wagenaar, Gerry T. M.; Markman, Marry; Lamers, W. H.; Destrée, Olivier; Clevers, Hans

doi:10.1128/mcb.18.3.1248

Cited by 303 publications

(243 citation statements)

References 45 publications

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“…In the microarray analysis, we observed a 55% increase in P2rx4 expression with age in +/sh2 heterozygotes, but only a 24% increase in sh2/sh2 mice. Tcf3 encodes a transcription factor implicated in Wnt/b-catenin signaling in early embryogenesis (Korinek et al 1998). In this study, Tcf3 showed a small but consistent decrease (14%) with age in sh2/sh2 mice, but no age-related change in +/sh2 mice.…”

Section: Genes With Age and Genotype Effectssupporting

confidence: 48%

“…b-Catenin and TCF3/LEF DNA-binding proteins form bipartitate complexes that are translocated to the nucleus to transactivate downstream genes, such as WISP1 (Xu et al 2000). In the absence of stabilized b-catenin, TCF3 can act as a repressor and plays a critical role in axis formation in the early embryo (Korinek et al 1998;Merrill et al 2004). WISP1 plays an important role as an osteoblastic regulator, promoting ostoblast differentiation and chondrocyte proliferation during embryonic skeletal development and repair of fractured bones (French et al 2004).…”

Section: Genes With Age-related Changesmentioning

confidence: 99%

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Age-Related Changes in Cochlear Gene Expression In Normal and Shaker 2 Mice

Gong

Károlyi

MacDonald

et al. 2006

JARO

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The vertebrate cochlea is a complex organ optimized for sound transduction. Auditory hair cells, with their precisely arranged stereocilia bundles, transduce sound waves to electrical signals that are transmitted to the brain. Mutations in the unconventional myosin XV cause deafness in both human DFNB3 families and in shaker 2 (sh2) mice as a result of defects in stereocilia. In these mutant mice, hair cells have relatively normal spatial organization of stereocilia bundles but lack the graded, stair-step organization. We used sh2 mice as an experimental model to investigate the molecular consequences of the sh2 mutation in the Myo15 gene. Gene expression profiling with Affymetrix GeneChips in deaf homozygous (sh2/sh2) mice at 3 weeks and 3 months of age, and in age-matched, normal-hearing heterozygotes (+/sh2) identified only a few genes whose expression was affected by genotype, but a large number with age-associated changes in expression in both normal mice and sh2/sh2 homozygotes. Microarray data analyzed using Robust Multiarray Average identified Aim1, Dbi, and Tm4sf3 as genes with increased expression in sh2/sh2 homozygotes. These increases were confirmed by quantitative reverse transcription-polymerase chain reaction. Genes exhibiting altered expression with age encoded collagens and proteins involved in collagen maturation, extracellular matrix, and bone mineralization.These results identified potential cellular pathways associated with myosin XV defects, and age-associated molecular events that are likely to be involved in maturation of the cochlea and auditory function.

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Section: Genes With Age and Genotype Effectssupporting

confidence: 48%

Section: Genes With Age-related Changesmentioning

confidence: 99%

Age-Related Changes in Cochlear Gene Expression In Normal and Shaker 2 Mice

Gong

Károlyi

MacDonald

et al. 2006

JARO

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“…19 The same study also indicated that SOX6 can suppress cyclin D1 activity by interacting with b-catenin and histone deacetylase 1. 20 However, using a TOPFlash (T-cell factor reporter plasmid) assay, 21 we were unable to demonstrate that SOX6 overexpression resulted in the suppression of wnt signaling activity.…”

Section: Discussionmentioning

confidence: 87%

Aberrant expression of microRNA 155 may accelerate cell proliferation by targeting sex‐determining region Y box 6 in hepatocellular carcinoma

Xie

Chen

et al. 2011

Cancer

107

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BACKGROUND: Recent research has suggested that the oncomir microRNA 155 (miR-155) is up-regulated in hepatocellular carcinoma (HCC). In this study, the authors investigated the tumorigenic mechanism of this oncomir in the development of HCC. METHODS: Quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) was conducted to analyze the expressions of miR-155 and its potential target genes in paired tumor tissues and adjacent tumor-free tissues and in disease-free liver tissue samples. The in silico predicted target genes of miR-155 were assessed by dual-luciferase reporting assay, real-time RT-PCR, and Western blot analyses. U6 promoters that drive miR-155 precursor overexpression and miR-155 tough decoy knock-down constructs were used to study its affects on cell proliferation in vitro and on tumor formation in nude mice. RESULTS: Quantitative RT-PCR demonstrated a gradual ascension of miR-155 expression in cirrhotic liver tissues and in HCC tumor tissues compared with low expression levels in normal liver tissues. Ectopic expression of miR-155 in HepG2 cells enhanced its tumorigenesis, whereas depletion of the endogenous miR-155 reversed these tumorigenic properties. Ectopic expression of sex-determining region Y box 6 (SOX6) was able to reverse the growth-promoting property of miR-155. Concordantly, the results demonstrated for the first time that SOX6 is a direct target of miR-155. Further analysis revealed that SOX6 reduced cell growth by up-regulating p21waf1/cip1 expression in a p53-dependent manner. In addition, a decline in p21waf1/ cip1 expression caused by miR-155 could be reversed by SOX6 expression. CONCLUSIONS: The current data indicated that SOX6 is a novel target of miR-155 and that miR-155 enhances liver cell tumorigenesis at least in part through the novel miR-155/SOX6/p21waf1/cip1 axis. These findings suggest that miR-155 may be a potential target for HCC treatment.

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“…However, the role of TLE in modulating Tcf4-dependent Wnt activity is controversial. A mouse TLE homolog does not bind to mouse Tcf4 in a yeast 2-hybrid assay, 57 and mouse and human Tcf4 share 98% amino acid homology; 58 however, a more recent study has demonstrated that TLE co-repressors could inhibit the transcriptional activity of all Tcf factors. 59 Further study is necessary to delineate the role, if any, of TLE factors in the de-repression of Wnt activity in butyrate-treated HCT-116 cells.…”

Section: Discussionmentioning

confidence: 94%

Cell type‐ and promoter‐dependent modulation of the Wnt signaling pathway by sodium butyrate

Bordonaro

Lazarova

Augenlicht

et al. 2001

Intl Journal of Cancer

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The Wnt signaling pathway modulates the transcription of genes linked to proliferation, differentiation and tumor progression. ␤-Catenin-Tcf (BCT)-dependent Wnt signaling is influenced by the short-chain fatty acid sodium butyrate, which induces growth arrest and/or maturation of colonic carcinoma cells. We have compared the effects of sodium butyrate on BCT-dependent signaling in 2 colon carcinoma cell lines that differ in their physiologic response to butyrate, with SW620 cells responding to butyrate by undergoing terminal differentiation and apoptosis, and HCT-116 cells undergoing reversible growth arrest, but no significant apoptotic cell death. Furthermore, these colon carcinoma cell lines differ in their mechanism of Wnt pathway activation, with adenomatous polyposis coli (APC) mutant SW620 cells having high levels of BCT complexes and APC wild-type HCT-116 cells having mutant ␤-catenin, low levels of BCT complexes and correspondingly higher levels of free Tcf. We have demonstrated that in SW620 cells, butyrate downregulates BCT-dependent expression of the Tcf-TK, matrilysin and cyclin D1 promoters, whereas in HCT-116 cells, butyrate upregulates expression of these promoters. Cotransfection with expression vectors that interfere with the Wnt pathway suggests that butyrate enhances BCT complex-DNA binding. Butyrate reduces the expression of Tcf4 in HCT-116 cells, consistent with the induction by butyrate of Tcf-repressible promoters in these cells. These findings indicate that sodium butyrate modulates the Wnt pathway in SW620 and HCT-116 cells in a different manner and that these differences have consequences for promoter activity that may influence the physiologic response to butyrate.

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Two Members of the Tcf Family Implicated in Wnt/β-Catenin Signaling during Embryogenesis in the Mouse

Cited by 303 publications

References 45 publications

Age-Related Changes in Cochlear Gene Expression In Normal and Shaker 2 Mice

Age-Related Changes in Cochlear Gene Expression In Normal and Shaker 2 Mice

Aberrant expression of microRNA 155 may accelerate cell proliferation by targeting sex‐determining region Y box 6 in hepatocellular carcinoma

Cell type‐ and promoter‐dependent modulation of the Wnt signaling pathway by sodium butyrate

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