Two major Spanish clones of penicillin-resistant Streptococcus pneumoniae in Portuguese isolates of clinical origin

Caniça, Manuela; Dias, Ricardo; Vaz-Pato, M. Vitória; Carvalho, Catarina

doi:10.1093/jac/dkg078

Cited by 6 publications

(5 citation statements)

References 7 publications

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“…Resistant strains of S. pneumoniae may be transported to geographically distant areas 26,27 . Importation of antibiotic-resistant organisms to Saudi Arabia may be facilitated by the presence of a large expatriate population and visitors to the Kingdom annually for pilgrimage 28 .…”

Section: Discussionmentioning

confidence: 99%

Pattern of Antibiotic Resistance ofStreptococcus pneumoniaein a Hospital in the Eastern Province of Saudi Arabia

Al‐Tawfiq

2004

Journal of Chemotherapy

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The objective of the study was to evaluate the pattern of antibiotic resistance of Streptococcus pneumoniae at Saudi Aramco Medical Services Organization (SAMSO) in the Eastern Province of Saudi Arabia. We identified, retrospectively, S. pneumoniae isolates from January 1999 to December 2002. Antimicrobial susceptibility and clinical data were collected and analyzed. A total of 162 isolates of S. pneumoniae were identified in the study period. Of these isolates, 94 (58%) isolates were obtained from out-patients and 68 (42%) were obtained from in-patients. Thirty-five percent of isolates were from blood, 46% from the respiratory tract, and the remainder were obtained from other sites. Of these isolates, 83 (51%) were penicillin-susceptible and the remaining 79 (48.8%) were not penicillin susceptible. High-level resistance to penicillin existed in 19.8% of the total isolates. Prevalence of resistance to erythromycin, tetracycline and trimethoprim-sulfamethoxazole was 25%, 29%, and 42% respectively. None of the isolates was resistant to vancomycin or ceftriaxone. However, 12% of the isolates showed multi-drug resistance. Streptococcus pneumoniae shows 19.8% high-level penicillin resistance and 12% multi-drug resistance. These findings call for wiser use of antibiotics.

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Section: Discussionmentioning

confidence: 99%

Pattern of Antibiotic Resistance ofStreptococcus pneumoniaein a Hospital in the Eastern Province of Saudi Arabia

Al‐Tawfiq

2004

Journal of Chemotherapy

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“…Erythromycin-resistant clone ST156 present in Portugal is a serotype 14 variant of the erythromycin-susceptible international clone Spain 9V À3 (Spain 9V À3-14). 9,11 This variant spread internationally, but most isolates are susceptible to macrolides, as identified in the MLST database (Figure 4). 10 ST156 found in this study has been described in different countries, but in Uruguay it was described as being resistant to erythromycin.…”

Section: Genotypic Characterizationmentioning

confidence: 99%

Emergence of invasive erythromycin-resistant Streptococcus pneumoniae strains in Portugal: contribution and phylogenetic relatedness of serotype 14

Dias¹,

Caniça²

2004

Journal of Antimicrobial Chemotherapy

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“…The penicillin sensitive strain Cp1015 was transformed with DNA from the serotype 9V resistant strain URA1258 related to the international multiresistant clone Spain 23F -1 [21]. Penicillin-resistant transformants were selected on plates containing 0.1 μg ml -1 of penicillin.…”

Section: Resultsmentioning

confidence: 99%

The highly conserved serine threonine kinase StkP of Streptococcus pneumoniae contributes to penicillin susceptibility independently from genes encoding penicillin-binding proteins

et al. 2009

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BackgroundThe serine/threonine kinase StkP of Streptococcus pneumoniae is a major virulence factor in the mouse model of infection. StkP is a modular protein with a N-terminal kinase domain a C-terminal PASTA domain carrying the signature of penicillin-binding protein (PBP) and prokaryotic serine threonine kinase. In laboratory cultures, one target of StkP is the phosphoglucosamine mutase GlmM involved in the first steps of peptidoglycan biosynthesis. In order to further elucidate the importance of StkP in S. pneumoniae, its role in resistance to β-lactams has been assessed by mutational analysis in laboratory cultures and its genetic conservation has been investigated in isolates from infected sites (virulent), asymptomatic carriers, susceptible and non-susceptible to β-lactams.ResultsDeletion replacement mutation in stkP conferred hypersensitivity to penicillin G and was epistatic on mutations in PBP2X, PBP2B and PBP1A from the resistant 9V clinical isolate URA1258. Genetic analysis of 55 clinical isolates identified 11 StkP alleles differing from the reference R6 allele. None relevant mutation in the kinase or the PASTA domains were found to account for susceptibility of the isolates. Rather the minimal inhibitory concentration (MIC) values of the strains appeared to be determined by their PBP alleles.ConclusionThe results of genetic dissection analysis in lab strain Cp1015 reveal that StkP is involved in the bacterial response to penicillin and is epistatic on mutations PBP 2B, 2X and 1A. However analysis of the clinical isolates did not allow us to find the StkP alleles putatively involved in determining the virulence or the resistance level of a given strain, suggesting a strong conservation of StkP in clinical isolates.

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Two major Spanish clones of penicillin-resistant Streptococcus pneumoniae in Portuguese isolates of clinical origin

Cited by 6 publications

References 7 publications

Pattern of Antibiotic Resistance ofStreptococcus pneumoniaein a Hospital in the Eastern Province of Saudi Arabia

Pattern of Antibiotic Resistance ofStreptococcus pneumoniaein a Hospital in the Eastern Province of Saudi Arabia

Emergence of invasive erythromycin-resistant Streptococcus pneumoniae strains in Portugal: contribution and phylogenetic relatedness of serotype 14

The highly conserved serine threonine kinase StkP of Streptococcus pneumoniae contributes to penicillin susceptibility independently from genes encoding penicillin-binding proteins

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