Two major autoantigen—Antibody systems of the mitotic spindle apparatus

Abstract

Objective. To characterize human autoantigenantibody systems related to the mitotic poles and spindles.Methods. Thirty-seven human sera with autoantibodies staining mitotic poles and spindles in indirect immunofluorescence (IIF) studies were further characterized by immunofluorescence on mitotic cells and by immunoblotting and immunoprecipitation. Clinical diagnoses meeting the American College of Rheumatology criteria were based on chart review and interview with the corresponding physicians.ResuUs. Two autoantibody systems reactive with mitotic poles and spindles were defined. Type 1 nuclear mitotic apparatus (NuMA-1) antibodies were identified in the serum of 30 patients. Interphase cells showed a fine, speckled, nuclear staining, while mitotic cells had bright staining of the rim of the centrosomes and light staining of the spindles proximal to the centrosomes. In telophase, the staining shifted from the centrosomes to the reforming nuclei. On immunoblotting, antiNuMA-1 sera reacted with a 210-kd protein. The reactivity of these sera was identified (with the aid of reference antibodies) as the previously described NuMA antigen-antibody system. Clinical information was available for only 17 of the 30 patients with antiNuMA-1; of these, 17 (53%) had clinical and lip biopsy findings that met the criteria for Sjiigren's syndrome.Publication 7668-MEM from The Scripps Research Institute.

Autoantibodies to a group of centrosomal proteins in human autoimmune sera reactive with the centrosome

“…In addition t o these antigens, which appear to b e integral components of the centrosome, the intranuclear protein NuMA (nuclear protein associated with the mitotic apparatus) is distributed t o daughter cells following cell division through its transient association with the centrosome (6)(7)(8). N u M A is an autoantigen that has been found to be frequently associated with Sjogren's syndrome (9).…”

The spindle kinesin‐like protein HsEg5 is an autoantigen in systemic lupus erythematosus

“…Based on the comparison of molecular weights and staining patterns, it became obvious that MA-I1 may be identical to HsEgS, 'while MA-I is the more abundant and well-characterided NuMA. To verify this correlation, we obtained 4 sera with MA-I1 activity from Dr. E. and colleagues that appears elsewhere in this issue of Arthritis and Rheumatism (27), MA-I was confirmed to be NuMA. These findings support our conclusion that NuMA and HsEgS are the predominant antigens responsible for the MSA pattern.…”

Human Autoantibodies to a Novel Golgi Protein Golgin-67: High Similarity With Golgin-95/gm 130 Autoantigen

“…It is therefore unlikely that the immune response is merely directed to crossreactive coiled-coils in these proteins. In addition, coiled-coils have been noted in many other non-Golgi autoantigens including nuclear mitotic apparatus protein NuMA [32,33], lamin B [34,35], myosin heavy chain [36], 52 kDa SS-A/Ro [37], and 70/80 kDa Ku antigens [38,39]. Our laboratories have collected and characterized human anti-Golgi sera by immunoblotting and immunoprecipitation over the past few years and in addition to the identified Golgi autoantigens reported, it was observed that many AGA sera recognized unidentified Golgi proteins (unpublished).…”