Two Lycopodium alkaloids from the aerial parts of Huperzia phlegmaria

Thu, Dang Kim; Vui, Dao Thi; Tùng, Bùi Thanh

doi:10.4103/pr.pr_82_19

Cited by 3 publications

(1 citation statement)

References 18 publications

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“…Lycopodiacea was used as traditional medicine in treatment for stroke, dementia for long time in Vietnam. Lycopodiacea family has wide variation and growth throughout of Vietnam [28,29], there are only a few researches on this topic, mainly focus on discover novel alkaloid and confirm chemical properties of found substances [11,30]. However, the lack of information on the effects of native species on animal model is a major setback.…”

Section: Introduction *mentioning

confidence: 99%

The Toxicity Evaluation of Lycopodiacea Extracts by Mice Neural Cell and Zebrafish Embryo

Kien¹,

Trang²,

Thanh³

et al. 2021

NST

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Many species in the family Lycopodiacea possess highly potential medical substances, notably are Huperzine A of Huperzia serrata and alkaloids isolated from Lycopodium clavatum(α-Onocerin , Lycopodine,..). Various members of this family are found throughout of Vietnam and a few researches on its diversity as well as alkaloid isolation has been conducted, however, there is a lack of toxicology studies on this subject. In this study, three crude extracts (labeled AI.1, AI.2 and AI.3), which come from Lycopodium sp.collected from 3 different regions of Vietnam, have been tested on 2 toxicological models: primary neural cells derived from Swiss white mouse (in vitro) and Zebrafish (Danio rerio) embryos (in vivo). Our results show that the all three extracts are relatively low in toxicity, IC50 value on primary neural cell ranging from 800 - 1100 mg/L, while LC50 of fish embryos at 100 - 300 mg/L. AI.2 has the least effect of viability of both cells and embryos compare to the others. This data give the prove showed that the safety using Lycopodium as tradional medicine.

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Section: Introduction *mentioning

confidence: 99%

The Toxicity Evaluation of Lycopodiacea Extracts by Mice Neural Cell and Zebrafish Embryo

Kien¹,

Trang²,

Thanh³

et al. 2021

NST

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The traditional uses, secondary metabolites, and pharmacology of Lycopodium species

Wang

Guan

2021

Phytochem Rev

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Differentiation Effect of Two Alkaloid Fractions from Vietnamese Lycopodiaceae on Mouse Neural Stem Cells

et al. 2022

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Various Lycopodium alkaloids have been studied for their various biological activities including anti-inflammatory, antioxidant, immunomodulatory, and neuroprotective activities. Moreover, these alkaloid compounds have high potential in the treatment of neuron degenerative disease. This study has been carried out to test the effect of Huperzia serrata (Thunb.) Trevis, and Lycopodium clavatum L alkaloid fractions on the mouse neural stem cells (NSCs). Firstly, the alkaloid fractions were used to verify its toxicity on NSCs. The multiple concentrations of alkaloid fractions from H. serrata (0.044; 0.088; 0.175; 0.35; 0.7; 1.4 mg/ml) and L. clavatum (0.031; 0.063; 0.125; 0.25; 0.50; 1.0; 2.0 mg/ml) have been used for the treatment of NSCs at period of 48h incubation. Results of the study suggested that the IC50 value of H. serrata and L. clavatum was 0.56 mg/ml and 0.50 mg/ml, respectively. Then, the NSCs were differentiated in the presence of 5 and 10 µg/ml of alkaloid fraction from H. serrata; 0.625 and 1.25 µg/ml of alkaloid fraction from L. clavatum for 6 days. Here, we observed the primary NSCs treated with alkaloid fraction extract from H. serrata showed the increased gene expression level of early neuron TUBB3 and neuron-specific cytoskeleton MAP2. On the other hand, the L. clavatum alkaloid fraction increased the expression of neural stem cell marker genes (Nestin and PAX6) and decreased neuron marker genes. In conclusion, these results established that alkaloid fraction from H. serrata promoted differentiation of the mouse NSCs to neuron cells, and L. clavatum extract had a capacity for stemness maintenance.

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Two Lycopodium alkaloids from the aerial parts of Huperzia phlegmaria

Cited by 3 publications

References 18 publications

The Toxicity Evaluation of Lycopodiacea Extracts by Mice Neural Cell and Zebrafish Embryo

The Toxicity Evaluation of Lycopodiacea Extracts by Mice Neural Cell and Zebrafish Embryo

The traditional uses, secondary metabolites, and pharmacology of Lycopodium species

Differentiation Effect of Two Alkaloid Fractions from Vietnamese Lycopodiaceae on Mouse Neural Stem Cells

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