Two Listeria monocytogenes Pseudo-outbreaks Caused by Contaminated Laboratory Culture Media

Matanock, Almea; Katz, Lee S.; Jackson, Kelly A.; Kučerová, Zuzana; Conrad, Amanda; Glover, William A.; Nguyen, Von; Mohr, Marika; Marsden-Haug, Nicola; Thompson, Deborah; Dunn, John R.; Stroika, Steven; Melius, Beth; Tarr, Cheryl L.; Dietrich, Stephen E.; Kao, Annie S.; Kornstein, Laura; Li, Zhen; Maroufi, Azarnoush; Marder, Ellyn; Meyer, Rebecca; Pérez-Osorio, Ailyn C.; Reddy, Vasudha; Reporter, Roshan; Carleton, Heather A.; Tweeten, Samantha; Waechter, HaeNa; Yee, Lisa M.; Wise, Matthew E.; Davis, Kim; Jackson, Brendan R

doi:10.1128/jcm.02035-15

Cited by 5 publications

(7 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…BioNumerics wgMLST was previously used to identify the source of an L. monocytogenes outbreak as contaminated laboratory culture media (204) and to determine the relatedness of 13 Y. pestis isolates (205).…”

Section: Complete Outbreak Analysis Software Suitesmentioning

confidence: 99%

Whole-Genome Sequencing of Bacterial Pathogens: the Future of Nosocomial Outbreak Analysis

et al. 2017

View full text Add to dashboard Cite

Outbreaks of multidrug-resistant bacteria present a frequent threat to vulnerable patient populations in hospitals around the world. Intensive care unit (ICU) patients are particularly susceptible to nosocomial infections due to indwelling devices such as intravascular catheters, drains, and intratracheal tubes for mechanical ventilation. The increased vulnerability of infected ICU patients demonstrates the importance of effective outbreak management protocols to be in place. Understanding the transmission of pathogens via genotyping methods is an important tool for outbreak management. Recently, whole-genome sequencing (WGS) of pathogens has become more accessible and affordable as a tool for genotyping. Analysis of the entire pathogen genome via WGS could provide unprecedented resolution in discriminating even highly related lineages of bacteria and revolutionize outbreak analysis in hospitals. Nevertheless, clinicians have long been hesitant to implement WGS in outbreak analyses due to the expensive and cumbersome nature of early sequencing platforms. Recent improvements in sequencing technologies and analysis tools have rapidly increased the output and analysis speed as well as reduced the overall costs of WGS. In this review, we assess the feasibility of WGS technologies and bioinformatics analysis tools for nosocomial outbreak analyses and provide a comparison to conventional outbreak analysis workflows. Moreover, we review advantages and limitations of sequencing technologies and analysis tools and present a real-world example of the implementation of WGS for antimicrobial resistance analysis. We aimed to provide health care professionals with a guide to WGS outbreak analysis that highlights its benefits for hospitals and assists in the transition from conventional to WGS-based outbreak analysis.

show abstract

Section: Complete Outbreak Analysis Software Suitesmentioning

confidence: 99%

Whole-Genome Sequencing of Bacterial Pathogens: the Future of Nosocomial Outbreak Analysis

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Listeria monocytogenes is the foodborne etiological agent of severe disease (listeriosis) in humans ( 1 – 3 ), which is typically confirmed by isolation of this bacterium from normally sterile sites such as blood ( 4 ). Strains from urine are uncommon ( 5 ).…”

Section: Announcementmentioning

confidence: 99%

“…Strains from urine are uncommon ( 5 ). L. monocytogenes from clinical specimens is typically isolated on agar media containing sheep blood ( 4 ). If the blood is contaminated with L. monocytogenes , e.g., when derived from animals with listeriosis, isolation of L. monocytogenes can be an artifact of the media, leading to pseudo-outbreaks ( 4 ).…”

Section: Announcementmentioning

confidence: 99%

“…L. monocytogenes from clinical specimens is typically isolated on agar media containing sheep blood ( 4 ). If the blood is contaminated with L. monocytogenes , e.g., when derived from animals with listeriosis, isolation of L. monocytogenes can be an artifact of the media, leading to pseudo-outbreaks ( 4 ). In 2013 and 2014, L. monocytogenes strains from non-sterile sites were found to constitute such pseudo-outbreaks ( 4 ).…”

Section: Announcementmentioning

confidence: 99%

See 1 more Smart Citation

Draft genome sequences of Listeria monocytogenes strains of sequence type 1733 which constituted a pseudo-outbreak due to contaminated blood agar media

Lee,

Sadat,

Kucerova

et al. 2024

Microbiol Resour Announc

Self Cite

View full text Add to dashboard Cite

show abstract

“…Besides, L. monocytogenes also has the ability to infect the laboratory, which was described as pseudo-outbreaks caused by contaminated laboratory culture media 7 . It was apparently clear that bacteria had been being evolved in unpredictable ways resulting in antibiotic resistance 8 and complicatedly occurred with the high frequency in current years 9 .…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Evaluation of Melaleuca alternifolia and Melaleuca citrina Essential Oils Against Listeria monocytogenes and Escherichia coli Applied in Disinfection

Ngan¹,

Trinh²,

Quy³

et al. 2023

Asian J. of Plant Sciences

View full text Add to dashboard Cite

Background and Objective: Bacterial contamination and consecutive infections had been highly concerned. Contamination has increasingly become prevalent which occurred in food, processing-food factories and laboratories resulting in severe consequences, more importantly, many dangerous diseases for humans. Thus, the investigation of natural compounds which are non-antibiotics, could inhibit the growth of pathogens and restrict the side effects to apply in disinfections as well as preventing bacterial contamination is imperative.The intention of this research was to study the antimicrobial activity of Melaleuca essential oils (consisting of Melaleuca alternifolia and Melaleuca citrina oils) which was also called tea tree oil (TTO), grounded on the chemical components. Materials and Methods: The bioactivity of TTO was evaluated by the combination of several methods including agar well diffusion and broth micro-dilution to determine minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs). The compositions of Melaleuca essential oils were identified by GC-MS analysis. Results: There were sixteen and twelve components obtained in M. alternifolia and M. citrina oils. The main compounds of M. alternifolia and M. citrina were eucalyptol (51.88 and 76.55%) and terpene-4-ol (30.08 and 4.93%), respectively. The Melaleuca oils showed high antibacterial activity which was assessed with two bacterial species representing Gram-positive and Gram-negative bacteria, Listeria monocytogenes and Escherichia coli. The antimicrobial efficacies were recorded at high values in L. monocytogenes with inhibition zones (13.67 and 16.00 mm), MICs were 0.2 and 1.25%, E. coli with inhibition zones (12.33 and 12.67 mm), MICs were 0.3 and 3.0% for M. alternifolia and M. citrina, respectively. Conclusion: The bioactivity of Melaleuca essential oils was proven that be effective in resisting both Gram-positive and Gram-negative bacteria. Thus, the TTO could be examined as a potential selection substitution for antibiotics.

show abstract

Two Listeria monocytogenes Pseudo-outbreaks Caused by Contaminated Laboratory Culture Media

Cited by 5 publications

References 4 publications

Whole-Genome Sequencing of Bacterial Pathogens: the Future of Nosocomial Outbreak Analysis

Whole-Genome Sequencing of Bacterial Pathogens: the Future of Nosocomial Outbreak Analysis

Draft genome sequences of Listeria monocytogenes strains of sequence type 1733 which constituted a pseudo-outbreak due to contaminated blood agar media

Antimicrobial Evaluation of Melaleuca alternifolia and Melaleuca citrina Essential Oils Against Listeria monocytogenes and Escherichia coli Applied in Disinfection

Contact Info

Product

Resources

About