Two linked pairs of Arabidopsis TNL resistance genes independently confer recognition of bacterial effector AvrRps4

Saucet, Simon B.; Ma, Yan; Sarris, Panagiotis F.; Furzer, Oliver J.; Sohn, Kee Hoon; Jones, Jonathan D. G.

doi:10.1038/ncomms7338

Cited by 158 publications

(163 citation statements)

References 65 publications

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“…S6B). RRS1B and RPS4B (Saucet et al, 2015) were present in all accessions, flanked by 12 differentially methylated TEs (Helitron and MuDR) (Fig. 7F and S6B).…”

Section: Resultsmentioning

confidence: 98%

Epigenomic Diversity in a Global Collection of Arabidopsis thaliana Accessions

Kawakatsu

Huang

Jupe

et al. 2016

Cell

614

923

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SUMMARY The epigenome orchestrates genome accessibility, functionality and three-dimensional structure. Because epigenetic variation can impact transcription and thus phenotypes, it may contribute to adaptation. Here we report 1,107 high-quality single-base resolution methylomes and 1,203 transcriptomes from the 1001 Genomes collection of Arabidopsis thaliana. Although the genetic basis of methylation variation is highly complex, geographic origin is a major predictor of genome-wide DNA methylation levels and of altered gene expression caused by epialleles. Comparison to cistrome and epicistrome datasets identifies associations between transcription factor binding sites, methylation, nucleotide variation and co-expression modules. Physical maps for nine of the most diverse genomes reveals how transposons and other structural variants shape the epigenome, with dramatic effects on immunity genes. The 1001 Epigenomes Project provides a comprehensive resource for understanding how variation in DNA methylation contributes to molecular and non-molecular phenotypes in natural populations of the most studied model plant.

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“…S6B). RRS1B and RPS4B (Saucet et al, 2015) were present in all accessions, flanked by 12 differentially methylated TEs (Helitron and MuDR) (Fig. 7F and S6B).…”

Section: Resultsmentioning

confidence: 98%

Epigenomic Diversity in a Global Collection of Arabidopsis thaliana Accessions

Kawakatsu

Huang

Jupe

et al. 2016

Cell

614

923

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“…On the other hand, Saucet et al. 20 suggested that A. thaliana accession RLD-0 lacks function of both RPS4/RRS1 and RPS4B/RRS1B. In this study, we showed that RPS4-Ws transferred RLD-0 plants, containing nonfunctional RPS4B/RRS1B, were resistant to the pathogens and RPS4-Ws Y950H complemented rps4-21 plants (Ws-2 background, containing functional RPS4B/RRS1B) were compatible to the pathogen.…”

Section: Discussionmentioning

confidence: 50%

“…On the other hand, Saucet et al. 20 reported that RPS4B ( At5g45060 )/ RRS1B ( At5g45050 ) is paralogous and functionally similar to RPS4 / RRS1 . RPS4B and RRS1B recognize AvrRps4 but not PopP2.…”

Section: Resultsmentioning

confidence: 99%

Analyses of natural variation indicates that the absence of RPS4/RRS1 and amino acid change in RPS4 cause loss of their functions and resistance to pathogens

Narusaka¹,

Iuchi

Narusaka³

2017

Plant Signaling & Behavior

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A pair of Arabidopsis thaliana resistance proteins, RPS4 and RRS1, recognizes the cognate Avr effector from the bacterial pathogens Pseudomonas syringae pv. tomato expressing avrRps4 (Pst-avrRps4), Ralstonia solanacearum, and the fungal pathogen Colletotrichum higginsianum and leads to defense signaling activation against the pathogens. In the present study, we analyzed 14 A. thaliana accessions for natural variation in Pst-avrRps4 and C. higginsianum susceptibility, and found new compatible and incompatible Arabidopsis–pathogen interactions. We first found that A. thaliana accession Cvi-0 is susceptible to Pst-avrRps4. Interestingly, the genome sequence assembly indicated that Cvi-0 lost both RPS4 and RRS1, but not RPS4B and RRS1B, compared to the reference genome sequence from A. thaliana accession Col-0. On the other hand, the natural variation analysis of RPS4 alleles from various Arabidopsis accessions revealed that one amino-acid change, Y950H, is responsible for the loss of resistance to Pst-avrRps4 and C. higginsianum in RLD-0. Our data indicate that the amino acid change, Y950H, in RPS4 resulted in the loss of both RPS4 and RRS1 functions and resistance to pathogens.

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“…RRS1B/RPS4 or RRS1‐R/RPS4B) leads to nonfunctional complexes, TIR domain swaps between these two protein pairs retain effector recognition function. This suggests that the TIR domains have similar roles for effector‐triggered activation in both paired NLRs (Saucet et al ., ). RRS1B and RRS1‐R TIR domains share ~70% identity at the amino acid level and the S25H26 motif (S22H23), C15 (C12) and P68 (P63) residues are located in highly conserved regions of RRS1‐R and RRS1B, respectively (Fig.…”

Section: Resultsmentioning

confidence: 97%

Autoimmunity and effector recognition in Arabidopsis thaliana can be uncoupled by mutations in the RRS1‐R immune receptor

et al. 2019

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Summary Plant nucleotide‐binding leucine‐rich repeat (NLR) disease resistance proteins recognize specific pathogen effectors and activate a cellular defense program. In Arabidopsis thaliana (Arabidopsis), Resistance to Ralstonia solanacearum 1 (RRS1‐R) and Resistance to Pseudomonas syringae 4 (RPS4) function together to recognize the unrelated bacterial effectors PopP2 and AvrRps4. In the plant cell nucleus, the RRS1‐R/RPS4 complex binds to and signals the presence of AvrRps4 or PopP2. The exact mechanism underlying NLR signaling and immunity activation remains to be elucidated. Using genetic and biochemical approaches, we characterized the intragenic suppressors of sensitive to low humidity 1 (slh1), a temperature‐sensitive autoimmune allele of RRS1‐R. Our analyses identified five amino acid residues that contribute to RRS1‐RSLH1 autoactivity. We investigated the role of these residues in the RRS1‐R allele by genetic complementation, and found that C15 in the Toll/interleukin‐1 receptor (TIR) domain and L816 in the LRR domain were also important for effector recognition. Further characterization of the intragenic suppressive mutations located in the RRS1‐R TIR domain revealed differing requirements for RRS1‐R/RPS4‐dependent autoimmunity and effector‐triggered immunity. Our results provide novel information about the mechanisms which, in turn, hold an NLR protein complex inactive and allow adequate activation in the presence of pathogens.

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Two linked pairs of Arabidopsis TNL resistance genes independently confer recognition of bacterial effector AvrRps4

Cited by 158 publications

References 65 publications

Epigenomic Diversity in a Global Collection of Arabidopsis thaliana Accessions

Epigenomic Diversity in a Global Collection of Arabidopsis thaliana Accessions

Analyses of natural variation indicates that the absence of RPS4/RRS1 and amino acid change in RPS4 cause loss of their functions and resistance to pathogens

Autoimmunity and effector recognition in Arabidopsis thaliana can be uncoupled by mutations in the RRS1‐R immune receptor

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